Carbon nanotube micropillars trigger guided growth of complex human neural stem cells networks

Lorite, Gabriela S.; Ylä‐Outinen, Laura; Janssen, Lauriane; Pitkänen, Olli; Joki, Tiina; Koivisto, Janne T.; Kellomäki, Minna; Vajtai, Róbert; Narkilahti, Susanna; Kordás, Krisztián

doi:10.1007/s12274-019-2533-2

Cited by 28 publications

(17 citation statements)

References 32 publications

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“…In addition, MWCNTs when stereotactically delivered to the mouse brain were found to reduce blood pressure drastically in the treated mouse [8]. Furthermore, carbon nanotube templates were used to effectively guide human neurite stem cell growth, and this mechanism may lead to improved therapeutic effects in the injured spinal cord [9].…”

Section: Introductionmentioning

confidence: 99%

Functionalized, Vertically Super-Aligned Multiwalled Carbon Nanotubes for Potential Biomedical Applications

Komane

Kumar

Choonara

et al. 2020

IJMS

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Currently, there is a lack of ultrasensitive diagnostic tool to detect some diseases such as ischemic stroke, thereby impacting effective and efficient intervention for such diseases at an embryonic stage. In addition to the lack of proper detection of the neurological diseases, there is also a challenge in the treatment of these diseases. Carbon nanotubes have a potential to be employed in solving the theragnostic challenges in those diseases. In this study, carbon nanotubes were successfully synthesized for potential application in the detection and treatment of the neurological diseases such as ischemic stroke. Vertically aligned multiwalled carbon nanotubes (VA-MWCNTs) were purified with HCl, carboxylated with H2SO4:HNO3 (3:1) and acylated with SOCl2 for use in potential targeting studies and for the design of a carbon-based electrode for possible application in the diagnosis of neurological diseases, including ischemic stroke. MWCNTs were washed, extracted from the filter membranes and dried in a vacuum oven at 60 °C for 24 h prior to functionalization and PEGylation. CNTs were characterized by SEM, TEM, OCA, DLS, CV and EIS. The HCl-treated CNT obtained showed an internal diameter, outer diameter and thickness of 8 nm, 34 nm and 75 µm, while these parameters for the H2SO4-HNO3-treated CNT were 8 nm, 23 nm and 41µm, respectively. PEGylated CNT demonstrated zeta potential, polydispersive index and particle size distribution of 6 mV, 0.41 and 98 nm, respectively. VA-MWCNTs from quartz tube were successfully purified, carboxylated, acylated and PEGylated for potential functionalization for use in targeting studies. For designing the carbon-based electrode, VA-MWCNTs on silicon wafer were successfully incorporated into epoxy resin for diagnostic applications. Functionalized MWCNTs were nontoxic towards PC-12 neuronal cells. In conclusion, vertically super-aligned MWCNTs have been successfully synthesized and functionalized for possible theragnostic biomedical applications in neurological disorders such as ischemic stroke.

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Section: Introductionmentioning

confidence: 99%

Functionalized, Vertically Super-Aligned Multiwalled Carbon Nanotubes for Potential Biomedical Applications

Komane

Kumar

Choonara

et al. 2020

IJMS

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“…[ 51 ] Biomaterials with pillar‐like characteristics have already been developed to investigate their effects on stem cells, including MSCs, [ 36 ] human adipose‐derived stem cells (hADSCs), [ 34 ] hematopoietic stem cells, [ 45 ] embryonic stem cells (ESCs), [ 51 ] and human neural stem cells (NSCs). [ 52 ] Pillars with high AR from the nanoscale to the microscale are inclined to reduce cell spreading but enhance elongation and differentiation. [ 34,36,51 ] Gadegaard et al.…”

Section: Biophysical Cues Of Biomaterials For Regulating Stem Cell Behaviormentioning

confidence: 99%

Manipulation of Stem Cells Fates: The Master and Multifaceted Roles of Biophysical Cues of Biomaterials

Wan

Liu

2021

Adv Funct Materials

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Owing to their self‐renewal and differentiation ability, stem cells are conducive for repairing injured tissues, making them a promising source of seed cells for tissue engineering. The extracellular microenvironment (ECM) is under dynamic mechanical control, which is closely related to stem cell behaviors. During the design and fabrication of biomaterials for regenerative medicine, the physiochemical properties of the natural ECM should be closely mimicked, which can reinforce stem cell lineage choice and tissue engineering. By reproducing the biophysical stimulations that stem cells may experience in vivo, many studies have highlighted the key role of biophysical cues in regulation of cell fate. Optimization of biophysical factors leads to desirable stem cell functions, which can maximize the effectiveness of regenerative treatment. In this review, the main biophysical cues of biomaterials, including stiffness, topography, mechanical force, and external physical fields are summarized, and their individual and synergistic influence on stem cell behavior is discussed. Subsequently, the current progress in tissue regeneration using biomaterials is presented, which directs the design and fabrication of functional biomaterial. The mechanisms via which biophysical cues activate cellular responses are also analyzed. Finally, the challenges in basic research as well as for clinical translation in this field are discussed.

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“…The results showed excellent anchoring in the formation of neural networks, which can be cultivated in different shapes and sizes, thereby being highly relevant in regenerative medicine. 101…”

Section: Carbon Nanotubesmentioning

confidence: 99%

Nanotechnology for the Treatment of Spinal Cord Injury

Zimmermann

Alves

Sperling

et al. 2021

Tissue Engineering Part B: Reviews

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Spinal cord injury (SCI) affects the central nervous system (CNS) and there is currently no treatment with the potential for rehabilitation. Although several clinical treatments have been developed, they are still at an early stage and have not shown success in repairing the broken fiber, which prevents cellular regeneration and integral restoration of motor and sensory functions. Considering the importance of nanotechnology and tissue engineering for neural tissue injuries, this review focuses on the latest advances in nanotechnology for SCI treatment and tissue repair. The PubMed database was used for the bibliographic survey. Initial research using the following keywords ''tissue engineering and spinal cord injury'' revealed 970 articles published in the last 10 years. The articles were further analyzed, excluding those not related to SCI or with results that did not pertain to the field of interest, including the reviews. It was observed that a total of 811 original articles used the quoted keywords. When the word ''treatment'' was added, 662 articles were found and among them, 529 were original ones. Finally, when the keywords ''Nanotechnology and spinal cord injury'' were used, 102 articles were found, 65 being original articles. A search concerning the biomaterials used for SCI found 700 articles with 589 original articles. A total of 107 articles were included in the discussion of this review and some are used for the theoretical framework. Recent progress in nanotechnology and tissue engineering has shown promise for repairing CNS damage. A variety of in vivo animal testing for SCI has been used with or without cells and some of these in vivo studies have shown successful results. However, there is no translation to humans using nanotechnology for SCI treatment, although there is one ongoing trial that employs a tissue engineering approach, among other technologies. The first human surgical scaffold implantation will elucidate the possibility of this use for further clinical trials. This review concludes that even though tissue engineering and nanotechnology are being investigated as a possibility for SCI treatment, tests with humans are still in the theoretical stage.

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Carbon nanotube micropillars trigger guided growth of complex human neural stem cells networks

Cited by 28 publications

References 32 publications

Functionalized, Vertically Super-Aligned Multiwalled Carbon Nanotubes for Potential Biomedical Applications

Functionalized, Vertically Super-Aligned Multiwalled Carbon Nanotubes for Potential Biomedical Applications

Manipulation of Stem Cells Fates: The Master and Multifaceted Roles of Biophysical Cues of Biomaterials

Nanotechnology for the Treatment of Spinal Cord Injury

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