Carbon Nanotube Delivery of the GFP Gene into Mammalian Cells

Gao, Lizeng; Nie, Leng; Wang, Taihong; Qin, Yujun; Guo, Zhixin; Yang, Di; Yan, Xiyun

doi:10.1002/cbic.200500227

Cited by 162 publications

(101 citation statements)

References 23 publications

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“…[10,11], probably because of a larger quantity of functional groups present on the surface of our CNTs. Moreover, our results are similar to those obtained with CNT functionalized by a cationic polymer, polyethyleneimine (PEI) [14].…”

Section: Transfectionsupporting

confidence: 86%

Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection

et al. 2009

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The possibility of delivering DNA effi ciently to cells represents a crucial issue for the treatment of both genetic and acquired diseases. However, even although the effi ciency of non-viral transfection systems has improved in the last decade, none have yet proven to be suffi ciently effective in vivo. We report herein our results on the functionalization of single-walled carbon nanotubes (SWNT) and multi-walled carbon nanotubes (MWNT) by two cationic amphiphiles (lipid RPR120535 and pyrenyl polyamine), their use for the complexation of plasmid DNA, and their efficiency in transfecting cells in vitro. The experiments have shown that the efficiency of transfection is higher when using SWNT instead of MWNT, and that transfection effi ciency is similar or slightly higher when using nanoplexes (SWNT/lipid RPR120535/DNA) instead of lipoplexes (lipid RPR120535/DNA) and several orders of magnitude higher than that of naked DNA. This study therefore shows both that the transfection is better when using SWNTs and that it is dependent on the nature of the amphiphilic molecules adsorbed on the nanotubes.

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Section: Transfectionsupporting

confidence: 86%

Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection

et al. 2009

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“…The most recent study in this exploratory exercise has been very recently published using GFP-encoding pDNA condensed with f -CNTs carrying positive charges [238]. In this way our initial reports and observations were indeed substantiated and reproduced in an independent study.…”

Section: 72supporting

confidence: 60%

Carbon Nanotube‐based Vectors for Delivering Immunotherapeutics and Drugs

Bianco

Pastorin

et al. 2003

Nanotechnologies for the Life Sciences

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“…The gene expression level by f-CNT-based DNA delivery was tenfold higher than for DNA alone. In addition, Gao et al 65 reported that amino-functionalized MWCNTs (NH 2 -MWCNTs) can interact with pDNA and they delivered the green fluorescence protein gene into cultured human cells. These results demonstrated the potential of pDNA delivery systems for various therapies.…”

Section: Cnts As Pdna Carriersmentioning

confidence: 99%

Carbon nanotubes as cancer therapeutic carriers and mediators

Son¹,

Hong²,

Lee³

2016

IJN

213

106

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Carbon nanotubes (CNTs) have received increasing attention in biomedical fields because of their unique structures and properties, including high aspect ratios, large surface areas, rich surface chemical functionalities, and size stability on the nanoscale. Particularly, they are attractive as carriers and mediators for cancer therapy. Through appropriate functionalization, CNTs have been used as nanocarriers for anticancer drugs including doxorubicin, camptothecin, carboplatin, cisplatin, paclitaxel, Pt(II), and Pt(IV), and genes including plasmid DNA, small-interfering RNA, oligonucleotides, and RNA/DNA aptamers. CNTs can also deliver proteins and immunotherapy components. Using combinations of light energy, they have also been applied as mediators for photothermal therapy and photodynamic therapy to directly destroy cancer cells without severely damaging normal tissue. If limitations such as a long-term cytotoxicity in the body, lack of size uniformity during the synthetic process, loading deviations for drug–CNT complexes, and release controllability at the target point are overcome, CNTs will become one of the strongest tools that are available for various other biomedical fields as well as for cancer therapy.

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Carbon Nanotube Delivery of the GFP Gene into Mammalian Cells

Cited by 162 publications

References 23 publications

Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection

Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection

Carbon Nanotube‐based Vectors for Delivering Immunotherapeutics and Drugs

Carbon nanotubes as cancer therapeutic carriers and mediators

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