In these works, we demonstrate that the optical properties of fluorescent carbon nanoparticles are not size-dependent. Fluorescent carbon nanoparticles with hydrodynamic diameters ranging from 10 -500 nm were prepared through variations of sugar source, concentration of agave (as a sugar source) and incubation time. Through comparisons made between these nanoparticles, we found no change in the local absorbance maxima and refractive index, with < 5 nm shifting in fluorescence maxima location.Because we were unable to observe significant changes in optical properties throughout a > 500 nm change in nanoparticle size, we can infer that the optical properties of fluorescent carbon nanoparticles are largely size-independent.