Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis

Lin, Xuexia; Su, Jianlong; Lin, Hung‐Mo; Zhou, Shu‐Feng; Sun, Xiaojie; Liu, Bin; Ming-rong, Zeng

doi:10.1038/s41598-019-40209-1

Cited by 8 publications

(4 citation statements)

References 22 publications

(18 reference statements)

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“…The CNPs were synthesized using microwave-assisted synthesis following the reported procedure [11,22]. Briefly, citric acid monohydrate (6 g) and urea (6 g) were dissolved by ultrasonication in 20 mL of water.…”

Section: Methodsmentioning

confidence: 99%

Using pH-Activable Carbon Nanoparticles as Cell Imaging Probes

Lin

Kankala

et al. 2019

Micromachines

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Herein, we demonstrate the fabrication of innovative pH-activable carbon nanoparticles (CNPs) based on urea and citric acid by microwave-assisted green synthesis for application in cell imaging. These CNP-based nanoprobes offer significant advantages of pH responsiveness and excellent biocompatibility. The pH responsiveness ranges from 1.0 to 4.6 and the slightly pH responsiveness ranges from 4.6 to 9.0. In addition, the pH-dependent modification of charge as well as the final diameter of the designed CNPs not only provide support as stable sensors for cell imaging under pH values from 4.6 to 9.0, but can also observe the pH change in cells from 1.0 to 4.6. Importantly, this significantly enhances the cellular internalization process resulting in tumor cell death. Together, we believe that these superior photoluminescence properties of our designed nanomaterials potentially allow for biological labeling, bioimaging, and drug delivery applications.

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Section: Methodsmentioning

confidence: 99%

Using pH-Activable Carbon Nanoparticles as Cell Imaging Probes

Lin

Kankala

et al. 2019

Micromachines

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“…Among the large family of nanomaterial-based optical sensors, carbon nanoparticles are some of the most used [ 47 ]. Our group has established a competitive analysis for antibiotic residues based on carbon nanoparticles (CNPs) and oligonucleotide probes [ 48 ]. In this work, the CNPs can be quenched by oxytetracycline (OTC) and can recover the luminescence through the application of OTC oligonucleotide probes, shown in Figure 6 A.…”

Section: Nanomaterial-based Optical Analysis Of Antibioticmentioning

confidence: 99%

“… Carbon nanoparticle-based fluorescence methods for the determination of antibiotics. ( A ) Competitive analysis for oxytetracycline, based on aptamer and carbon nanoparticles [ 48 ]. ( B ) Microplate-based fluorescence method for kanamycin detection based carbon nanoparticles [ 49 ].…”

Section: Figurementioning

confidence: 99%

A Minireview for Recent Development of Nanomaterial-Based Detection of Antibiotics

Hong

Zhao

et al. 2023

Biosensors

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Antibiotics are considered a new type of organic pollutant. Antibiotic residues have become a global issue due to their harm to human health. As the use of antibiotics is increasing in human life, such as in medicine, crops, livestock, and even drinking water, the accurate analysis of antibiotics is very vital. In order to develop rapid and on-site approaches for the detection of antibiotics and the analysis of trace-level residual antibiotics, a high-sensitivity, simple, and portable solution is required. Meanwhile, the rapid nanotechnology development of a variety of nanomaterials has been achieved. In this review, nanomaterial-based techniques for antibiotic detection are discussed, and some reports that have employed combined nanomaterials with optical techniques or electrochemical techniques are highlighted.

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“…To prove the suitability of SA/pNIPAAm hydrogel for drug delivery system, the degradation of SA/pNIPAAm hydrogels and the drug entrapment efficiency (EP%) were evaluated. Since OTC has been extensively used as an antibiotic and growth promoter [27], it was loaded as the model drug in this study. For the drug release study, the release kinetics of OTC were performed at 37 °C with pH values ranging from 1.91 to 11.65.…”

Section: Introductionmentioning

confidence: 99%

Dual-Responsive Alginate Hydrogels for Controlled Release of Therapeutics

Lin

et al. 2019

Molecules

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In this work, with the drug oxytetracycline (OTC) released, cell cytotoxicity and antimicrobial studies of dual-responsive sodium alginate and N-Isopropylacrylamide hydrogels (SA/pNIPAAm) with enclosed OTC were investigated. The molecular OTC release was explored with different acid-base conditions and temperature conditions. In order to characterize cell cytotoxicity and antimicrobial efficacy, time-dependent OTC release analysis of different acid-base conditions was performed in SA/pNIPAAm hydrogels. OTC@SA/pNIPAAm hydrogels showed excellent time-dependent antimicrobial efficacy, in which the IC50 values were 50.11 μg mL−1, 34.27 μg mL−1, and 22.39 μg mL−1 among three consecutive days, respectively. Meanwhile, the human cells showed excellent viability at the IC50 dosage of OTC@SA/pNIPAAm (50.11 μg mL−1). OTC@SA/pNIPAAm performed in this study indicated that SA/pNIPAAm may serve as drug carriers for sustainable release at a specific concentration and for being employed as substrates for decreasing drug toxicity. Besides, pH-responsive and thermos-responsive SA/pNIPAAm may lead to the better selectivity of drug release in the ideal location or site. Finally, the results demonstrate that the designed, dual-responsive, biocompatible OTC@SA/pNIPAAm hydrogels showed excellent antimicrobial efficacy and may potentially be found to have enormous applicability in the field of pharmaceutics.

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Carbon nanoparticles with oligonucleotide probes for a label-free sensitive antibiotic residues detection based on competitive analysis

Cited by 8 publications

References 22 publications

Using pH-Activable Carbon Nanoparticles as Cell Imaging Probes

Using pH-Activable Carbon Nanoparticles as Cell Imaging Probes

A Minireview for Recent Development of Nanomaterial-Based Detection of Antibiotics

Dual-Responsive Alginate Hydrogels for Controlled Release of Therapeutics

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