Abstract:Cystic fibrosis is an autosomal recessive disorder caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) that can lead to terminal respiratory failure. Ultrafine carbonaceous particles, which are ubiquitous in ambient urban and indoor air, are increasingly considered as major contributors to the global health burden of air pollution. However, their effects on the expression of CFTR and associated genes in lung epithelial cells have not yet been investigated. We therefore evaluat… Show more
CRISPR-mediated genome editing enables to study disease-relevant mutations. Next Generation Sequencing offers high throughput and accuracy but requires substantial investment. Nanopore sequencing on the other hand provides cheap entry. Here, we describe our work using sequencing techniques to identify edited cells, analyze mitochondrial DNA, and gene expression. Finally, we describe how our software tools CRISPRnano and Duesselpore aid to democratize the use of modern sequencing technologies.
CRISPR-mediated genome editing enables to study disease-relevant mutations. Next Generation Sequencing offers high throughput and accuracy but requires substantial investment. Nanopore sequencing on the other hand provides cheap entry. Here, we describe our work using sequencing techniques to identify edited cells, analyze mitochondrial DNA, and gene expression. Finally, we describe how our software tools CRISPRnano and Duesselpore aid to democratize the use of modern sequencing technologies.
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