Carbon Nanohorns as a Scaffold for the Construction of Disposable Electrochemical Immunosensing Platforms. Application to the Determination of Fibrinogen in Human Plasma and Urine

Ojeda, Irene; Garcinuño, Belit; Moreno‐Guzmán, María; González‐Cortés, Araceli; Yudasaka, Masako; Iijima, Sumio; Langa, Fernando; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1021/ac501681n

Cited by 55 publications

(24 citation statements)

References 37 publications

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“…In recent years it has been proven that electrode material and structure play critical roles to achieve simple, sensitive, rapid, and stable detection in bioanalysis using electrochemical or piezoelectrical methods [1][2][3]. Nanomaterials and three-dimensional (3D) nanostructures have been introduced into electrode structures to improve the performance of electrical measurements [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Wang

Callaway

et al. 2017

Sensors and Actuators B: Chemical

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In this study, a cost-effective nanowell structure was fabricated and utilized for the development of a nanowell-based quartz crystal microbalance (QCM) aptasensor for rapid, sensitive, and label-free detection of H5N1 avian influenza virus (AIV). A nanoporous gold film with a thickness of 120 nm and a pore size of ~20 nm was prepared using a metallic corrosion method. Then, the nanoporous gold film was immobilized onto a gold electrode surface using a self-assembled monolayer to form a nanowell-based electrode. A specific H5N1 AIV ssDNA aptamer with a NH 2 conjugated 5'-terminal was used in the fabrication of the QCM aptasensor through covalent bonding. The stepwise assembly of the aptasensor was characterized by means of QCM. The result showed that the binding of target AIV H5N1 onto the immobilized aptamers decreased the sensor's resonant frequency, and the frequency change correlated to the virus titer. We demonstrated that the developed nanowell-based QCM aptasensor could dramatically reduce detection time down to 10 min using a label-free assay. The detection range of 2-4 to 2 4 hemagglutination units (HAUs) /50 μl was obtained with a detection limit of 2-4 HAU/50 μl for AIV H5N1. The binding of target H5N1 virus onto the nanowell-based electrode surface was further confirmed by scanning electron microscopy (SEM). No interference was observed from non-target AIV subtypes of H1N1, H2N2, H7N2 and H5N3. The aptasensor using H5N1 aptamer was validated for the detection of AIV H5N1 in chicken tracheal swab samples. The developed aptasensor could be adopted for detection of other viruses.

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Section: Introductionmentioning

confidence: 99%

“…increases the surface area by 245 mm2 or ((265.4/20.4)×100) = 1,300%. During the immobilization procedure, MHDA and HDT at a ratio of 1:1 were applied, therefore, only half of the gold surface area was functionalized with carboxylic functional groups for further NH 2apatmer immobilization.…”

mentioning

confidence: 99%

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Wang

Callaway

et al. 2017

Sensors and Actuators B: Chemical

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“…[197] These devices were fabricated by the immobilization of fibrinogen onto activated CNHs deposited on screen-printed carbon electrodes. Ojeda et al developed simple and relatively low-cost immune sensors for determination of fibrinogen in human plasma and urine.…”

Section: Cnms For Biomedical Applicationsmentioning

confidence: 99%

Green Processing of Carbon Nanomaterials

Kawamoto

2016

Advanced Materials

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Carbon nanomaterials (CNMs) from fullerenes, carbon nanotubes, and graphene are promising carbon allotropes for various applications such as energy-conversion devices and biosensors. Because pristine CNMs show substantial van der Waals interactions and a hydrophobic nature, precipitation is observed immediately in most organic solvents and water. This inevitable aggregation leads to poor processability and diminishes the intrinsic properties of the CNMs. Highly toxic and hazardous chemicals are used for chemical and physical modification of CNMs, even though efficient dispersed solutions are obtained. The development of an environmentally friendly dispersion method for both safe and practical processing is a great challenge. Recent green processing approaches for the manipulation of CNMs using chemical and physical modification are highlighted. A summary of the current research progress on: i) energy-efficient and less-toxic chemical modification of CNMs using covalent-bonding functionality and ii) non-covalent-bonding methodologies through physical modification using green solvents and dispersants, and chemical-free mechanical stimuli is provided. Based on these experimental studies, recent advances and challenges for the potential application of green-processable energy-conversion and biological devices are provided. Finally, a conclusion section is provided summarizing the insights from the present studies as well as some future perspectives.

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“…Then, an indirect competitive assay with horseradish peroxidase (HRP)‐labeled anti‐Fib and using hydroquinone (HQ) as redox mediator, was implemented. As a feature to be highlighted, the Fib‐CNHs/SPCEs exhibited an excellent storage stability of at least 42 days .…”

Section: Carbon Nanohornsmentioning

confidence: 99%

Uncommon Carbon Nanostructures for the Preparation of Electrochemical Immunosensors

et al. 2016

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1IntroductionCarbon nanomaterials such as single-or multi-walled nanotubes and grapheneh ave been extensively used as electrode modifiers in the preparation of electrochemical sensors andb iosensors.E lectrode nanostructuration with these carbon materials allows preparing scaffolds with improved conductivity and selectivity and with enhanced ability for the immobilization of biomolecules. In addition, carbon nanomaterials can be used to designs uitable strategies for signala mplificationt hus leading to significant improvements in sensitivity.M ore recently,a part from the above mentioned carbon nanomaterials,o ther forms of carbon nanoforms have emergeda sn ovel nanostructuration tools suitable to be employed also in the preparation of novel electrochemical sensors and biosensor designs achieving improved analytical performance. Carbon nanohorns,d ouble-walled carbon nanotubes,f ullerene (C60), carbon nanoparticles,a nd graphene quantum dots are examples of thesen ovel nanomaterials whose particular characteristics make them appropriate for the constructiono fb ioelectrochemical devices.I nt his review article,t he use of these carbon nanomaterials in the particular field of electrochemical immunosensors preparation is reviewed.Thea bove mentioned, so called, uncommon carbon nanomaterials have been employed in the designo fe lectrochemical immunoassays in order to enhance their analytical performance both from the standpoint of attain am ore effective and simple immobilization of immunoreagents as to get betterd etection limits.A si tw ill be commentedb elow,t his analytical enhancementc an be achieved by taking advantage of somes pecific properties of these materialss uch as the ability of carbon nanohornst o provide numerous surface confined carboxylg roups for covalent binding, or the excellent capability of fullerene (C60) to accelerate electron transfer,a sw ell as its ability for combination with other materials to improve biocompatibility or water solubility.A lthough there are other carbon nanomaterials which have been scarcely explored for applications in electrochemical biosensing, only those of these nanomaterials that have demonstrated already their usefulness for the preparation of electrochemical immunosensors have been considered in this article. 2Carbon NanohornsSingle-walled carbon nanohorns,S WCNHs (or simply CNHs) are ar elatively new type of carbon allotrope.O ne single CNH consists of au nique horn-shaped graphene sheet with al ength of 40-50 nm and ad iameter ranging between 2a nd 5nm ( Figure 1a) [1].C NHs are prepared by vaporizing pure graphite rods via CO 2 laser ablation without usinga ny metalc atalysts.T hus,a ni mportant advantage of this materials over i.e.c arbon nanotubes is that CNHs are essentially metal-free [2, 3] and can be used directly without post-treatment. Typically,C NHs assembles to form nanostructures similar to dahlia flowers composed of several units (Figure 1b) with ad iameter near from 100 nm [4,5].T he special properties of CNHs derive from the high conductivity...

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Carbon Nanohorns as a Scaffold for the Construction of Disposable Electrochemical Immunosensing Platforms. Application to the Determination of Fibrinogen in Human Plasma and Urine

Cited by 55 publications

References 37 publications

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Green Processing of Carbon Nanomaterials

Uncommon Carbon Nanostructures for the Preparation of Electrochemical Immunosensors

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