Carbon nanodots as fluorescence probes for rapid, sensitive, and label-free detection of Hg<sup>2+</sup>and biothiols in complex matrices

Zhou, Li; Lin, Youhui; Huang, Zhenzhen; Ren, Jinsong; Qu, Xiaogang

doi:10.1039/c2cc16791c

Cited by 674 publications

(358 citation statements)

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“…As we know, Hg(II), Cu(II), and Fe(III) ions are a class of oxidant which can quench the fluorescence of CNPs due to facilitating non-radiative electron/hole recombination annihilation through an effective electron transfer process. [36] The quenching effects of these metal ions on multicolor CNPs emission could be explored for the development of smart sensor in nanotechnology and nano-devices [37].…”

Section: Metal Ions Effects On Fluorescence Intensitymentioning

confidence: 99%

Development of multicolor carbon nanoparticles for cell imaging

Huang

Tan

Zhang

et al. 2013

Talanta

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Section: Metal Ions Effects On Fluorescence Intensitymentioning

confidence: 99%

Development of multicolor carbon nanoparticles for cell imaging

Huang

Tan

Zhang

et al. 2013

Talanta

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“…Materials like lipids, natural and synthetic polymers, carbohydrates, surfactants and dendrimers are used as drug carriers [1][2][3] . The drug conjugate can be designed for improving its potential for complex - interactions towards the target moiety and drug [4][5][6][7] .…”

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confidence: 99%

One-Step Microwave-Assisted Green Synthesis of Luminescent N-Doped Carbon Dots from Sesame Seeds for Selective Sensing of Fe(III)

Roshni¹,

Divya²

2017

Current Science

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The present study focuses on the green synthesis of nitrogen-doped carbon dots (C.dots) from sesame seeds using microwave pyrolysis method. The C.dots obtained were characterized and extensively studied using transmission electron microscopy (TEM), UV/ visible spectroscopy, fluorescence spectroscopy and Fourier transform infra-red spectroscopy (FTIR) techniques. The results indicated the presence of highly fluorescent, aqueous soluble and significantly photostable C.dots with a quantum yield of 8.02%. The average size distributions of C.dots were found to be 5 nm. These C.dots were effectively applied as a selective sensor for Fe(III) as the fluorescence intensity was significantly quenched with increasing metal concentrations. The limit of detection (LOD) was found to be 2.56 M of Fe(III). This study demonstrates a low cost, environmental friendly and waste recyclable synthetic method for preparation of C.dots and its application as a selective Fe(III) sensor.Keywords: Carbon dots, fluorescence, sesame seeds, microwave pyrolysis, metal ions, quenching.CARBON DOTS (C.dots) are gaining increasing attention within the carbon nanomaterial family due to their varied applications in biological, sensing, optical and electrical fields 1-3 . They are swiftly replacing the conventional semiconductor nanoparticles (Q.dots) which have raised serious health and environmental concerns because of the presence of heavy metals in it 4 . C.dots are spherical, oxygenous carbon nanoparticles with size below 10 nm. C.dots akin to Q.dots show several interesting properties such as excitation-dependent emission, chemical and photostability, enhanced fluorescence quantum yields and the possibility of fine-tuning the sensing properties by surface modification [5][6][7][8][9][10][11] In order to improve the photophysical and photochemical properties of C.dots, hetero-atom doped C.dots are synthesized by different ways. Among them, nitrogendoped C.dots received much attention because of their good biocompatibility and the related broad applications in the areas of bio-imaging, electrocatalysis and sensors 25,26 . However, most of the synthesis methods reported so far have either a long reaction time or a need of extra chemicals for doping. Thus, synthesizing Ndoped C.dots in a cost effective and ecofriendly manner and using them for sensing applications, especially for biologically active and environmentally hazardous metal ions will be highly beneficial.Iron is an essential element for many biological processes 27 . The presence of iron in body either at higher levels or at lower levels can lead to several problems which eventually disturbs the lipid metabolism and glucose levels 28 . Therefore, quantitative determination of bioactive iron is an important health care challenge. Some studies have already been reported in this direction; Qian et al. 29 used the N-doped C.dots for detection of Fe(III). Wang et al. 30 used a wide variety of green carbonaceous precursors like milk, silk, honey, hair, lemon, etc. to detect Fe(III).The pre...

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“…To validate the universal chemiluminescent nature of the carbon dots in the presence of a strong alkaline solution, carbon dots were prepared by pyrolysis of ethylenediaminetetraacetic acid (EDTA) salts, 19 and similar CL behaviour was accompanied with the addition of different concentrations of NaOH (Fig. S3B in the ESI †).…”

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Chemiluminescence of carbon dots under strong alkaline solutions: a novel insight into carbon dot optical properties

et al. 2013

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We report the surprising chemiluminescence (CL) behavior of fluorescent carbon dots in the presence of a strong alkaline solution, such as NaOH or KOH. The CL intensity was dependent on the concentration of the base and carbon dots in a certain range. A possible CL mechanism was studied by UV-Vis, fluorescence, CL, FTIR, XPS and EPR spectroscopy. Radiative recombination of the injected electrons by "chemical reduction" of carbon dots with thermally excited generated holes was proposed, which sheds new light on the characteristics of carbon dots.As a rising star of the nanocarbon family, carbon nanodots have attracted increasing attention due to their excellent luminescent properties and wide applications in many areas of fundamental and technical importance.1 Intense research still focuses on their further application and intrinsic luminescence mechanism.Luminescent properties of carbon dots are usually investigated by photoluminescence (PL) produced using photoexcitation, 2-8 and electrochemiluminescence (ECL) generated by electron injection. 9-12However, chemiluminescence (CL) generated from chemical energy excitation through a chemical reaction has been rarely used to study the optical properties of carbon dots. To the best of our knowledge, only Lin 13-15 and Cui's 16 groups have recently described the CL behavior of carbon dots when they coexist with oxidants (KMnO 4 , Ce(IV), NaIO 4 ) or an ultraweak chemiluminescence system (H 2 O 2 -NaHSO 3 , H 2 O 2 -HNO 2 ) under acidic conditions. Herein, we observed for the rst time that CL was generated when only a strongly alkaline solution was injected into the carbon dots, without the presence of any CL reagent, CL system or oxidants. Our initial purpose was to study the CL behavior of carbon dots using an alkaline solution as a reaction medium to improve the CL intensity. Surprisingly, the addition of high concentration NaOH solution to the carbon dots was accompanied by a fast CL phenomenon. This investigation provided new insight into the physical-chemical and optical properties of carbon dots.The carbon dots with a diameter of 2-4 nm (Fig. S1 in the ESI †) were synthesized by the microwave treatment described in the literature with a slight modication (see ESI †), using glucose as the carbon source and PEG 1500 as the co-reactant.9 As with the other carbon dots, the synthesized nanomaterial exhibited a UV-Vis absorption band at 280 nm, attributed to the n-p* transition of the C]O band and a p-p* transition of the conjugated C]C band.17 The uorescence (FL) emission shied as the excitation wavelength increased. When the emission reached its maximum, the peak centered at 525 nm. Interestingly, with the addition of NaOH into the carbon solution, the colour of the carbon dot aqueous solution darkened from light yellow to yellow-brown. A new absorption peak at 272 nm appeared in the UV-Vis spectrum, but no appreciable absorption peak was observed under 280 nm for the original carbon Fig. 1 The UV-Vis absorption spectra of pristine carbon dots and NaOH-treated c...

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Carbon nanodots as fluorescence probes for rapid, sensitive, and label-free detection of Hg²⁺and biothiols in complex matrices

Abstract: In this work, unmodified carbon nanodots are demonstrated as novel and environmentally-friendly fluorescence probes for the sensing of Hg(2+) and biothiols with high sensitivity and selectivity.

Cited by 674 publications

References 39 publications

Development of multicolor carbon nanoparticles for cell imaging

Development of multicolor carbon nanoparticles for cell imaging

One-Step Microwave-Assisted Green Synthesis of Luminescent N-Doped Carbon Dots from Sesame Seeds for Selective Sensing of Fe(III)

Chemiluminescence of carbon dots under strong alkaline solutions: a novel insight into carbon dot optical properties

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Carbon nanodots as fluorescence probes for rapid, sensitive, and label-free detection of Hg2+and biothiols in complex matrices

Abstract: In this work, unmodified carbon nanodots are demonstrated as novel and environmentally-friendly fluorescence probes for the sensing of Hg(2+) and biothiols with high sensitivity and selectivity.

Cited by 674 publications

References 39 publications

Development of multicolor carbon nanoparticles for cell imaging

Development of multicolor carbon nanoparticles for cell imaging

One-Step Microwave-Assisted Green Synthesis of Luminescent N-Doped Carbon Dots from Sesame Seeds for Selective Sensing of Fe(III)

Chemiluminescence of carbon dots under strong alkaline solutions: a novel insight into carbon dot optical properties

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Carbon nanodots as fluorescence probes for rapid, sensitive, and label-free detection of Hg²⁺and biothiols in complex matrices