Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins

d’Amora, Marta; Maffeis, Viviana; Brescia, Rosaria; Barnes, Danielle; Scanlan, Eoin M.; Giordani, Silvia

doi:10.3390/nano9081069

Cited by 19 publications

(19 citation statements)

References 20 publications

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“…We recently reported the non-covalent functionalization of carbon nano-onions (CNOs; a multilayer fullerene-like graphitic CNM) with highly fluorescent pyrene–BODIPY dyads and their application for biomedical imaging, with promising results (Bartelmess et al, 2015 ). These findings comply with other recent works, where CNOs exhibited a prompt and effective uptake by different cell lines, (Frasconi et al, 2015 ; Lettieri et al, 2017a , b ; d'Amora et al, 2019 ) revealing high stability, low toxicity, and high biocompatibility in zebrafish during their development (d'Amora et al, 2016 , 2017 ).…”

Section: Introductionsupporting

confidence: 93%

Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions

et al. 2020

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Photodynamic therapy is currently one of the most promising approaches for targeted cancer treatment. It is based on responses of vital physiological signals, namely, reactive oxygen species (ROS), which are associated with diseased condition development, such as tumors. This study presents the synthesis, incorporation, and application of a diiodo-BODIPY–based photosensitizer, based on a non-covalent functionalization of carbon nano-onions (CNOs). In vitro assays demonstrate that HeLa cells internalize the diiodo-BODIPY molecules and their CNO nanohybrids. Upon cell internalization and light exposure, the pyrene–diiodo-BODIPY molecules induce an increase of the ROS level of HeLa cells, resulting in remarkable photomediated cytotoxicity and apoptosis. Conversely, when HeLa cells internalize the diiodo-BODIPY/CNO nanohybrids, no significant cytotoxicity or ROS basal level increase can be detected. These results define a first step toward the understanding of carbon nanomaterials that function as molecular shuttles for photodynamic therapeutics, boosting the modulation of the photosensitizer.

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Section: Introductionsupporting

confidence: 93%

Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions

et al. 2020

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“…Following overnight incubation, the medium was removed and replaced with fresh DMEM, containing various concentrations (0.5, 1, 5, and 10 µg/mL) of BN-CNOs and oxi-BN-CNOs for 72 h. Cells were incubated with 5% dimethyl sulfoxide (DMSO is toxic to any kind of cells) as a positive control, while cells with the fresh medium were used as a negative control. The cytotoxicity was assessed using WST-1 assay (Roche Applied Science, Mannheim, Germany), according to our previous report [4]. This simple colorimetric assay was based on the conversion of the tetrazolium salt WST-1 in a colored dye by cellular mitochondrial dehydrogenases enzymes.…”

Section: Cell Culturementioning

confidence: 99%

“…Carbon nano-onions (CNOs) are a member of the carbon family consisting of a multi nested fullerene-like structure [1] and a size ranging from a few to tens of nm depending on the synthetic methodology [2]. In the last decade, CNOs have shown great promise in bio-related applications due to their small size, high surface area, and easy surface modification approaches, as well as low in vitro cytotoxicity [3,4] and excellent in vivo biocompatibility [4,5]. In particular, their remarkable emission properties, high cellular uptake, and low cytotoxicity of different fluorescent-labelled CNOs allowed their use as bioimaging probes in several cell lines [3,6,7].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Biocompatibility of Boron/Nitrogen Co-Doped Carbon Nano-Onions

et al. 2021

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Boron/nitrogen, co-doped, carbon nano-onions (BN-CNOs) have recently shown great promise as catalysts for the oxygen reduction reaction, due to the improved electronic properties imparted by the dopant atoms; however, the interactions of BN-CNOs with biological systems have not yet been explored. In this study, we examined the toxicological profiles of BN-CNOs and oxidized BN-CNOs (oxi-BN-CNOs) in vitro in both healthy and cancer cell lines, as well as on the embryonic stages of zebrafish (Danio rerio) in vivo. The cell viabilities of both cell lines cells were not affected after treatment with different concentrations of both doped CNO derivatives. On the other hand, the analysis of BN-CNOs and oxidized BN-CNO interactions with zebrafish embryos did not report any kind of perturbations, in agreement with the in vitro results. Our results show that both doped CNO derivatives possess a high biocompatibility and biosafety in cells and more complex systems.

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“…Also, both Gly- and BSA-conjugated CNOs can be readily internalised compared to unconjugated Gly and BSA. This indicates the ability of CNOs as non-toxicity carriers [ 74 ]. Although, CNOs are remarkable in biomedical applications, investigations of their potential on SCs remain scarce.…”

Section: Carbon As Scaffold Biomaterials For Sc Applicationsmentioning

confidence: 99%

Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application—A Review

2021

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A scaffold is a crucial biological substitute designed to aid the treatment of damaged tissue caused by trauma and disease. Various scaffolds are developed with different materials, known as biomaterials, and have shown to be a potential tool to facilitate in vitro cell growth, proliferation, and differentiation. Among the materials studied, carbon materials are potential biomaterials that can be used to develop scaffolds for cell growth. Recently, many researchers have attempted to build a scaffold following the origin of the tissue cell by mimicking the pattern of their extracellular matrix (ECM). In addition, extensive studies were performed on the various parameters that could influence cell behaviour. Previous studies have shown that various factors should be considered in scaffold production, including the porosity, pore size, topography, mechanical properties, wettability, and electroconductivity, which are essential in facilitating cellular response on the scaffold. These interferential factors will help determine the appropriate architecture of the carbon-based scaffold, influencing stem cell (SC) response. Hence, this paper reviews the potential of carbon as a biomaterial for scaffold development. This paper also discusses several crucial factors that can influence the feasibility of the carbon-based scaffold architecture in supporting the efficacy and viability of SCs.

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Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins

Cited by 19 publications

References 20 publications

Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions

Modulation of Efficient Diiodo-BODIPY in vitro Phototoxicity to Cancer Cells by Carbon Nano-Onions

In Vitro and In Vivo Biocompatibility of Boron/Nitrogen Co-Doped Carbon Nano-Onions

Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application—A Review

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