Carbon monoxide releasing molecule enhances coagulation and decreases fibrinolysis in canine plasma exposed to <i>Crotalus viridis</i> venom in vitro and in vivo

Johnson, Theodore; Wells, Robert L.; Bell, Amy; Nielsen, Vance G.; Olver, Christine S.

doi:10.1111/bcpt.13242

Cited by 9 publications

(4 citation statements)

References 35 publications

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“…Сполуки-донори також проявили себе як потужні активатори ангіогенезу, а також як інгібітори прозапальних ферментів, вазодилататори. Вони знижували активацію тромбоцитів, зменшували ступінь окисного стресу, проявляли антиапоптотичні властивості [7,8].…”

Section: вступunclassified

The Carbon Monoxide Donor, Topiramate, and Blockers of Aquaporine Receptors Decrease Myocardial Ischemia-Reperfusion Injiry

Beschasnyi¹,

Hasiuk²

2021

Fiziol Zh

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We investigated the metabolism of mouse isolated heart under the influence of tricarbonyldichlorothenium (II)- dimer (CORM-2 and 2,3-4,5-bis-O-isopropylidene-βD-fructopyranose sulfamate (topiramate) as potential blockers of aquaporine channel (AQP3) of cardiac myocytes. The results were compared with those obtained from the group receiving anti-AQP3 monoclonal antibodies. A decrease in coronary flow was found during the period preceding ischemia (topiramate did not cause this effect). However, at the end of reperfusion, CORM-2 was responsible for its stabilization. This compound did not affect glucose intake (topiramate increased it only at the end of reperfusion), decreased Ca2+ deposition in cardiac muscle (AQP3-IgG antibodies and topiramate had similar effect), decreased creatinine release, AST (especially at the end of reperfusion). The action of CORM-2 increased the amplitude of the R waveform before ischemia and during reperfusion. At the end of reperfusion the R-wave amplitude decreased. The effect of topiramate caused an increase in amplitude only at the beginning of reperfusion. Administration of CORM-2, topiramate and antibodies resulted in prolongation of the interval before and during ischemia. At the same time, the effect of these drugs and antibodies reduced the development of ischemic damage. The results indicate that the released CO from CORM-2 has effects similar to those of anti-AQP3 antibodies. The action of topiramate had signs of calcium channel blocking.

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Section: вступunclassified

The Carbon Monoxide Donor, Topiramate, and Blockers of Aquaporine Receptors Decrease Myocardial Ischemia-Reperfusion Injiry

Beschasnyi¹,

Hasiuk²

2021

Fiziol Zh

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“…На клітинній лінії кардіоміоцитів щура H9c2 було виявлено протекторний вплив СО від ішеміїреперфузії шляхом інгібування Ca 2+ каналів L-типу, що захищало їх від «перенавантаження» кальцієм, проте призводило до активації кальпаїну. Блокада спричиняла зниження потреби клітин у енергії, необхідній для скорочення, що у свою чергу відтерміновувало ішемічну загибель [14].…”

Section: вплив низьких концентрацій монооксиду вуглецю на метаболізм ізольованого серця в умовах ішемії-реперфузії херсонський державний unclassified

Influence of Low Concentrations of Carbon Monoxide on Metabolism of Isolated Heart under Conditions of Ischemia-Reperfusion

Beschasnyi¹,

Lysenko²

2021

Ukr. ž. med. bìol. sportu

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The purpose of the study was to determine the effect of different concentrations of carbon monoxide on the metabolism of isolated mice hearts. Materials and methods. To elucidate the effect of low concentrations of carbon monoxide on the myocardium, we performed retrograde perfusion of isolated hearts of laboratory mice with Krebs-Henseleit solution, which was saturated with carbon monoxide for 5, 10, and 30 minutes. We then determined how different concentrations of carbon monoxide affected coronary volumetric flow rate, myocardial glucose and calcium uptake, creatinine release, and aspartate aminotransferase release. During perfusion, R-wave amplitude and R-R interval were measured using an electrocardiograph. To determine the effect of ischemia on the heart muscle during perfusion with solutions of different concentrations, we measured the area of the affected myocardium after staining with 2,3,5-triphenyltetrazolium chloride. Results and discussion. After these studies, it was found that different concentrations of carbon monoxide had a dose-dependent effect on the isolated mouse heart. However, the dependence of the effects does not follow the pattern «lowest concentration – lowest effect». At the same time, an increase in concentration did not mean an increase in adverse effects on the myocardium. Even on the contrary, the smallest concentration led to increased signs of ischemic myocardial damage. In particular, the use of the solution, through which carbon monoxide was passed for 5 minutes, caused vasoconstrictor effect during perfusion. At the end of reperfusion, vasoconstrictor effect was observed after using a solution through which carbon monoxide was passed for 10 minutes. Increased glucose uptake was observed in the group with 30-minute carbon monoxide permeation against the background of the minimal myocardial creatinine release. In this group there was also a decrease in Ca2+ loss at the beginning of reperfusion (immediately after ischemia). The above phenomenon explains the least degree of ischemic myocardial damage in the isolated mouse heart. The obtained data should be expanded. Since it is difficult to accurately determine the dose of carbon monoxide, then the use of donor compounds is promising. Such compounds include CORM-2 and CORM-3. Under physiological conditions, they decompose in a controlled manner, releasing a specific amount of carbon monoxide. Conclusion. The obtained results indicate that at different concentrations of carbon monoxide can differently influence different structures of cardiomyocyte: at one concentration it binds to calcium channels, other concentrations influence ion channels of plasma membrane, which can explain all these dependencies

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“…Recent studies have explored the use of TEG modified by the addition of tissue plasminogen activator (tPA) to the reaction mixture to standardize initiation of clot lysis. [4][5][6][7][8][9][10] A study investigating the relationship between spontaneous hemoperitoneum in dogs and hemostatic abnormalities employed a tPA-modified whole-blood TEG assay.…”

Section: Introductionmentioning

confidence: 99%

Comparison of coagulation and fibrinolysis in Irish Wolfhounds and age‐matched control dogs using tissue plasminogen activator‐augmented viscoelastic testing

Davis,

Fletcher,

Newman

et al. 2024

J Vet Emergen Crit Care

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ObjectiveTo determine if Irish Wolfhounds (IWs), like other sighthounds, are hyperfibrinolytic compared with nonsighthound dogs using 2 native and tissue plasminogen activator (tPA)‐enhanced viscoelastic assays, one that is whole blood‐based (viscoelastic coagulation monitor [VCM]) and the other that is plasma‐based thromboelastography (TEG).DesignCohort study.SettingUniversity teaching hospital.AnimalsA convenience sample of 27 IWs recruited from the Irish Wolfhound Association of New England Specialty and the local community, and 27 healthy, age‐matched, large‐breed control dogs.InterventionsNone.Measurements and Main ResultsBlood samples including CBC, biochemistry, traditional coagulation, and viscoelastic testing were collected from IWs and control dogs. Twelve IWs had viscoelastic testing. IWs had lower fibrinogen concentrations (215.5 ± 57.8 vs 251.4 ± 64.5 mg/dL, P = 0.034) and formed weaker clots on both whole‐blood VCM and plasma TEG assays (maximum clot firmness [VCM‐MCF] = 39.4 [25.1–48.8] vs 48.5 [34.6–57.3], P = 0.0042; maximum amplitude [TEG‐MA] = 22.7 [14.7–33.6] vs 32.2 [26.9–42.0], P < 0.0001). IWs were hyperfibrinolytic compared with control dogs on VCM whole‐blood assays, with 25 U/mL tPA (lysis at 30 min [VCM‐LI30] = 68.1 [0–100] vs\ 99.9 [63.3–100], P = 0.0009; lysis at 45 min [VCM‐LI45] = 31.0 [0–100] vs 98.1 [38.4–100], P = 0.0002) but hypofibrinolytic compared with controls on TEG plasma assays with 50 U/mL tPA (lysis at 30 min [TEG‐LY30] = 45.7 [4.6–94.6] vs 93.7 [12.3–96.5], P = 0.0004; lysis at 60 min [TEG‐LY60] = 68.7 [29.7–96.8] vs 95.7 [34.4–97.6], P = 0.0003). Minimal fibrinolysis was measured on whole‐blood VCM or plasma TEG assays without the addition of tPA, and there were no differences between the 2 groups.ConclusionsWeaker clots were found in IWs than control dogs. With the addition of tPA, IWs had evidence of hyperfibrinolysis on whole‐blood VCM assays and hypofibrinolysis on plasma TEG assays compared with control dogs. Without the addition of tPA, however, both groups of dogs showed minimal fibrinolysis on viscoelastic testing.

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Carbon monoxide releasing molecule enhances coagulation and decreases fibrinolysis in canine plasma exposed to Crotalus viridis venom in vitro and in vivo

Cited by 9 publications

References 35 publications

The Carbon Monoxide Donor, Topiramate, and Blockers of Aquaporine Receptors Decrease Myocardial Ischemia-Reperfusion Injiry

The Carbon Monoxide Donor, Topiramate, and Blockers of Aquaporine Receptors Decrease Myocardial Ischemia-Reperfusion Injiry

Influence of Low Concentrations of Carbon Monoxide on Metabolism of Isolated Heart under Conditions of Ischemia-Reperfusion

Comparison of coagulation and fibrinolysis in Irish Wolfhounds and age‐matched control dogs using tissue plasminogen activator‐augmented viscoelastic testing

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