Carbon monoxide metabolism in cell suspensions of Peptostreptococcus productus strain Marburg

Ma, Kuo-Jian

doi:10.1016/0378-1097(87)90428-9

Cited by 2 publications

(3 citation statements)

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“…Among the acetogenic bacteria, Peptostreptococcus productus (Lorowitz & Bryant 1984;Geerligs et al 1987;Ma et al 1987;Ma et al 1991) is the species tolerating the highest CO concentrations. Strains of this species were described to grow in the presence of 90% (v/v) (Lorowitz & Bryant 1984) and 50-70% (v/v) CO (Geerligs et al 1987) in the gas phase, respectively.…”

Section: Acetogenic Bacteriamentioning

confidence: 99%

Microbial growth on carbon monoxide

et al. 1992

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The utilization of carbon monoxide as energy and/or carbon source by different physiological groups of bacteria is described and compared. Utilitarian CO oxidation which is coupled to the generation of energy for growth is achieved by aerobic and anaerobic eu-and archaebacteria. They belong to the physiological groups of aerobic carboxidotrophic, facultatively anaerobic phototrophic, and anaerobic acetogenic, methanogenic or sulfate-reducing bacteria. The key enzyme in CO oxidation is CO dehydrogenase which is a molybdo iron-sulfur flavoprotein in aerobic CO-oxidizing bacteria and a nickel-containing iron-sulfur protein in anaerobic ones. In carboxidotrophic and phototrophic bacteria, the CO-born CO2 is fixed by ribulose bisphosphate carboxylase in the reductive pentose phosphate cycle. In acetogenic, methanogenic, and probably in sulfate-reducing bacteria, CODH/acetyl-CoA synthase directly incorporates CO into acetyl-CoA.In plasmid-harbouring carboxidotrophic bacteria, CO dehydrogenase as well as enzymes involved in CO2 fixation or hydrogen utilization are plasmid-encoded. Structural genes encoding CO dehydrogenase were cloned from carboxidotrophic, acetogenic and methanogenic bacteria. Although they are clustered in each case, they are genetically distinct.Soil is a most important biological sink for CO in nature. While the physiological microbial groups capable of CO oxidation are well known, the type and nature of the microorganisms actually representing this sink are still enigmatic. We also tried to summarize the little information available on the nutritional and physicochemical requirements determining the sink strength. Because CO is highly toxic to respiring organisms even in low concentrations, the function of microbial activities in the global CO cycle is critical.

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Section: Acetogenic Bacteriamentioning

confidence: 99%

Microbial growth on carbon monoxide

et al. 1992

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“…Since acetate synthesis from both substrates (CO and H2) involves a different pathway for the formation of the carboxyl group [10], it must be assumed that sodium plays a role in the reduction of CO 2 to the methyl group. From the finding, that formate as an intermediate in methyl group synthesis is accumulated in the absence of sodium, it is concluded, that the sodium dependent reaction is involved in the conversion of formate to the methyl group of acetate.…”

Section: Discussionmentioning

confidence: 99%

“…CO is oxidized to CO 2 by the carbon monoxide dehydrogenase (CODH), thus serving as an electron donor for the reduction of CO 2 to the methyl group. The methyl group is further carbonylated directly to an acetyl group with CO as the carbonyl donor [10]. The reduction of methylene tetrahydrofolate with CO as the electron donor is even more exergonic (AG o'= -78 kJ/mol) (see 'framed' reaction in Fig.…”

Section: Peptostreptococcus Productus (Strain Marburg) Ismentioning

confidence: 99%

Sodium dependent acetate formation from CO2 in Peptostreptococcus productus (strain Marburg)

Geerligs

Schönheit

Diekert

1989

FEMS Microbiology Letters

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Washed cells of Peptostreptococcus productus (strain Marburg), which were incubated in the presence of CO/CO2/N2 (50%/ 17%/ 33%; 200 kPa) catalyzed the synthesis of acetate from carbon monoxide. The rate of acetate formation from CO was stimulated more than threefold by the addition of sodium (10 mM); potassium did not effect acetate synthesis. The degree of stimulation was dependent on the sodium concentration; the dependence followed simple Michaelis‐Menten kinetics. The apparent Km for sodium was determined to be about 2 mmol/1. Sodium also stimulated acetate synthesis from H2 plus CO2. In the absence of added sodium the formation of formate as an intermediate in methyl group synthesis was stimulated. It is suggested that the sodium dependent reaction(s) is one (or more) of the reactions involved in methyl group synthesis from CO2.

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Carbon monoxide metabolism in cell suspensions of Peptostreptococcus productus strain Marburg

Cited by 2 publications

References 0 publications

Microbial growth on carbon monoxide

Microbial growth on carbon monoxide

Sodium dependent acetate formation from CO2 in Peptostreptococcus productus (strain Marburg)

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