Carbon metabolism of C<sup>14</sup>-labeled amino acids in wheat leaves. I. A pathway of glyoxylate-serine metabolism

Wang, Dalton; Waygood, E. R.

doi:10.1104/pp.37.6.826

Cited by 57 publications

(26 citation statements)

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“…In prior investigations of the transformation of glycine to sugars in wheat leaves, a pathway of glyoxylate-serine metabolism was proposed (23,24), and a similar pathway also has been described by Rabson et al (19). Although evidence reported so far has favored the operation of this pathway in wheat leaves, a number of questions previously could not be resol-ed wNithout further experimental evidence.…”

Section: Discussionmentioning

confidence: 99%

“…This often has been assumed to occur in plants, because (24), neither glycolate nor glyoxylate lowered the C'4 in sugars when glycine-C14 was the substrate.…”

Section: Discussionmentioning

confidence: 99%

“…McConnell and Bilinski (13) and( Tolbert (22) demonstrated that formate could serve as a source of the /8-carbon of serine in wheat and barley plants. The slow conversion of glycine to glyoxylate (24) makes it difficult to visualize how glyoxylate can serve as a source of one-carbon units for the rapid synthesis of serine. The extent of conversion of the a-carbon of glycine to the ,8-carbon of serine is reflected in the amount of C14 in carbons 1 and 6 of glucose formed from glycine-2-C'4 (24).…”

Section: Discussionmentioning

confidence: 99%

“…The isolation of glucose by paper chromatography as well as the acid hydrolysis of sucrose and the subsequent isolation of the glucose moiety were performed according to the procedures described previously (24).…”

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confidence: 99%

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Carbon Metabolism of C¹⁴-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Wang

Burris

1963

Plant Physiol.

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Section: Discussionmentioning

confidence: 99%

“…This often has been assumed to occur in plants, because (24), neither glycolate nor glyoxylate lowered the C'4 in sugars when glycine-C14 was the substrate.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Carbon Metabolism of C¹⁴-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Wang

Burris

1963

Plant Physiol.

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“…Hence, it seemed unlikely that the decarboxylation of glyoxylate could occur by such a mechanism in the peroxisomes, although it might occur elsewhere in the cell. They suggested instead (6,7) that the glycolate pathway (11,15) must first produce glycine (Fig. 1), and that the CO2 is released during the complex hydroxymethyltransferase condensation of two glycine molecules to produce serine.…”

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confidence: 99%

Comparison of the Effectiveness of Glycolic Acid and Glycine as Substrates for Photorespiration

Zelitch

1972

Plant Physiol.

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Considerable evidence exists that the carboxyl-carbon atom of glycolic acid is the primary source of the C02 produced during photorespiration by leaves of many species of plants, including tobacco. Experiments were conducted to determine whether glyoxylate or glycine, both products of glycolic acid metabolism, is the more immediate precursor of photorespiratory C02.Illuminated tobacco leaf disks were floated on 18 mMsolutions of glycolate-1-YC or glycine-1-C in COrfree air. The "CO2 released and the "C content of several postulated intermediates were determined when the substrate solutions were provided alone or with one of the following: 9 mM a-hydroxy-2-pyridinemethanesulfonic acid, an inhibitor of the oxidation of glycolate to glyoxylate; 9 mM isonicotinyl hydrazide, an inhibitor of the conversion of glycine to serine; or 18 mmnonradioactive glycine or glycolate with the other radioactive substrate.Both inhibitors decreased the rate of photorespiration in tobacco leaf disks by the "C-assay. The a-hydroxy-2-pyridinemethanesulfonic acid severely blocked '4CO2 production and labeling of the glycolate pathway from glycolate-1-"C. Isonicotinyl hydrazide had little effect on the "CO2 released from glycine-l-'4C although the glycine to serine conversion was severely inhibited.These results and other data in the literature indicate that the glycolate pathway of carbohydrate metabolism does not supply sufficient C02 during the synthesis of serine from glycine to account for the rates of photorespiration observed in many species. A direct decarboxylation of glyoxylate is more likely the main source of photorespiratory C02.Ample evidence exists that the large quantities of CO2 produced in many photosynthetic tissues in the light arise primarily from the carboxyl-carbon atom of glycolic acid, an early product of photosynthesis (1, 2). This evidence comes from experiments showing parallel effects on glycolate metabolism and photorespiration brought about by changes in the oxygen and CO2 concentrations in the ambient atmosphere, the use of biochemical inhibitors and "C-labeled metabolites on intact tissues, and assays of enzyme activities in leaf extracts and in isolated leaf organelles (4,5,23 (ref. 23, pp. 204-208).

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Genetic and Environmental Control of Glycolic Acid Oxidase Activity in Ecotypic Populations of Typha Latifolia

McNaughton

1969

American J of Botany

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Glycolic acid oxidase activity at 25 C in leaf homogenates of ecotypic populations of Typha latifolia varied according to native climate of the population and current growing conditions. Activity of plants grown under warm/short day conditions was positively correlated with maximum summer temperature at the site of population origin. Activity of plants grown under cool/ long day conditions was negatively correlated with length of growing season at the site of origin. Populations from sites characterized by a long growing season are much less susceptible to environmental regulation of enzymic level than populations originating in short growing season sites. Enzyme activity is not a secondary reflection of differentiation at the level of chlorophyll or cofactor concentration. Enzymic differences between ecotypic populations reflect differences in control systems at the level of protein synthesis and genetic structure.

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Carbon metabolism of C¹⁴-labeled amino acids in wheat leaves. I. A pathway of glyoxylate-serine metabolism

Cited by 57 publications

References 5 publications

Carbon Metabolism of C¹⁴-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Carbon Metabolism of C¹⁴-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Comparison of the Effectiveness of Glycolic Acid and Glycine as Substrates for Photorespiration

Genetic and Environmental Control of Glycolic Acid Oxidase Activity in Ecotypic Populations of Typha Latifolia

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Carbon metabolism of C14-labeled amino acids in wheat leaves. I. A pathway of glyoxylate-serine metabolism

Cited by 57 publications

References 5 publications

Carbon Metabolism of C14-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Carbon Metabolism of C14-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Comparison of the Effectiveness of Glycolic Acid and Glycine as Substrates for Photorespiration

Genetic and Environmental Control of Glycolic Acid Oxidase Activity in Ecotypic Populations of Typha Latifolia

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Carbon metabolism of C¹⁴-labeled amino acids in wheat leaves. I. A pathway of glyoxylate-serine metabolism

Carbon Metabolism of C¹⁴-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism

Carbon Metabolism of C¹⁴-Labeled Amino Acids in Wheat Leaves. II. Serine & its Role in Glycine Metabolism