Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study

Suetens, Annelies; Moreels, Marjan; Quintens, Roel; Chiriotti, S.; Tabury, Kevin; Michaux, Arlette; Grégoire, Vincent; Baatout, Sarah

doi:10.3892/ijo.2014.2287

Cited by 33 publications

(36 citation statements)

References 72 publications

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“…However, during Hadron-therapy they can be very effective against tumour growth (Suetens et al 2014). ATP depletion also inhibits the growth of tumour cell lines (Lu et al 2000), and chemicals decreasing ATP levels have radiosensitizing effect (Kumar et al 1993), whereas exogenous ATP is protective against neutron irradiation (Szeinfeld and De Villiers 1992).…”

Section: Discussionmentioning

confidence: 99%

Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons

Dionet

Müller-Barthélémy

Marceau

et al. 2016

International Journal of Radiation Biology

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Melanoma cells or fibroblasts exert their own survival behaviour at very low doses of neutrons, suggesting that in some cases there is a differential between cancer and normal cells radiation responses. Only the survival of fibroblasts at HDR fits the linear no-threshold model. This new insight into human cell responses to very low doses of neutrons, concerns natural radiations, surroundings of accelerators, proton-therapy devices, flights at high altitude. Furthermore, ATP inhibitors could increase HRS during high-linear energy transfer (high-LET) irradiation.

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Section: Discussionmentioning

confidence: 99%

Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons

Dionet

Müller-Barthélémy

Marceau

et al. 2016

International Journal of Radiation Biology

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“…Hadrontherapy with carbon ions is more effective than conventional radiotherapy in inducing DNA damage, cell cycle arrest and cell death in tumor cells [ 5 – 7 ], which accounts for the highly lethal effects. Because the fate of irradiated cells is believed to be controlled by a network of signaling pathways, we recently investigated the effects of a different radiation types on genome-wide gene expression in human prostate adenocarcinoma (PC3) cells [ 8 ]. We performed a microarray study on PC3 cells after carbon ion irradiation and X-irradiation and observed a downregulation in several motility-related genes at 8 h after exposure [ 8 ] that was more pronounced after carbon ion irradiation compared with X-irradiation.…”

Section: Introductionmentioning

confidence: 99%

Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation

Suetens

Moreels

Quintens

et al. 2014

Journal of Radiation Research

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Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness (RBE). This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses (0, 0.5 and 2 Gy) of accelerated 13C-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines. However, whereas in PC3 cells the response to carbon ion irradiation was enhanced compared with X-irradiation, the effect was the opposite in Caco-2 cells, indicating cell-type–specific responses to the different radiation types.

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“…High-LET radiation exposure was performed using the D1 IRABAT high-energy scanning beam line at the Grand Accélérateur National d’Ions Lourds (GANIL, Caen, France). The dosimetry and calibration beam was done by CIMAP (Centre de Recherche sur les Ions, les Matériaux et la Photonique) as previously described [ 22 , 23 ]. Accelerated 18 O ions (50 MeV/a, < 0.1 nA) were used at a dose rate of ~1 Gy/min which corresponded to a mean fluency of 1.22 × 10 5 ions/ (cm 2 .s).…”

Section: Methodsmentioning

confidence: 99%

In vitro engineering of human 3D chondrosarcoma: a preclinical model relevant for investigations of radiation quality impact

et al. 2015

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BackgroundThe benefit of better ballistic and higher efficiency of carbon ions for cancer treatment (hadron-therapy) is asserted since decades, especially for unresectable or resistant tumors like sarcomas. However, hadron-therapy with carbon ions stays underused and raises some concerns about potential side effects for patients. Chondrosarcoma is a cartilaginous tumor, chemo- and radiation-resistant, that lacks reference models for basic and pre-clinical studies in radiation-biology. Most studies about cellular effects of ionizing radiation, including hadrons, were performed under growth conditions dramatically different from human homeostasis. Tridimensional in vitro models are a fair alternative to animal models to approach tissue and tumors microenvironment.MethodsBy using a collagen matrix, standardized culture conditions, physiological oxygen tension and a well defined chondrosarcoma cell line, we developed a pertinent in vitro 3D model for hadron-biology studies. Low- and high-Linear Energy Transfer (LET) ionizing radiations from GANIL facilities of ~1 keV/μm and 103 ± 4 keV/μm were used respectively, at 2 Gy single dose. The impact of radiation quality on chondrosarcoma cells cultivated in 3D was analyzed on cell death, cell proliferation and DNA repair.ResultsA fair distribution of chondrosarcoma cells was observed in the whole 3D scaffold. Moreover, LET distribution in depth, for ions, was calculated and found acceptable for radiation-biology studies using this kind of scaffold. No difference in cell toxicity was observed between low- and high-LET radiations but a higher rate of proliferation was displayed following high-LET irradiation. Furthermore, 3D models presented a higher and longer induction of H2AX phosphorylation after 2 Gy of high-LET compared to low-LET radiations.ConclusionsThe presented results show the feasibility and usefulness of our 3D chondrosarcoma model in the study of the impact of radiation quality on cell fate. The observed changes in our tissue-like model after ionizing radiation exposure may explain some discrepancies between radiation-biology studies and clinical data.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-015-1590-5) contains supplementary material, which is available to authorized users.

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Carbon ion irradiation of the human prostate cancer cell line PC3: A whole genome microarray study

Cited by 33 publications

References 72 publications

Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons

Different dose rate-dependent responses of human melanoma cells and fibroblasts to low dose fast neutrons

Dose- and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation

In vitro engineering of human 3D chondrosarcoma: a preclinical model relevant for investigations of radiation quality impact

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