Carbon flow in an upland grassland: effect of liming on the flux of recently photosynthesized carbon to rhizosphere soil

Rangel‐Castro, J. Ignacio; Prosser, James I.; Scrimgeour, C. M.; Smith, Pete; Ostle, Nicholas J.; Ineson, Phil; Meharg, Andrew A.; Killham, Ken

doi:10.1111/j.1365-2486.2004.00883.x

Cited by 47 publications

(46 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The 13 C isotope signal is then tracked in shoots, roots, and rhizosphere soil during the months following the pulse. In grassland sites, very rapid turnover of C has been observed (Rangel-Castro et al 2004). …”

Section: Measuring Changes In Carbon In the Soilmentioning

confidence: 99%

Sectoral approaches to improve regional carbon budgets

et al. 2008

Self Cite

View full text Add to dashboard Cite

Humans utilise about 40% of the earth's net primary production (NPP) but the products of this NPP are often managed by different sectors, with timber and forest products managed by the forestry sector and food and fibre products from croplands and grasslands managed by the agricultural sector. Other significant anthropogenic impacts on Climatic Change (2008) the global carbon cycle include human utilization of fossil fuels and impacts on less intensively managed systems such as peatlands, wetlands and permafrost. A great deal of knowledge, expertise and data is available within each sector. We describe the contribution of sectoral carbon budgets to our understanding of the global carbon cycle. Whilst many sectors exhibit similarities for carbon budgeting, some key differences arise due to differences in goods and services provided, ecology, management practices used, landmanagement personnel responsible, policies affecting land management, data types and availability, and the drivers of change. We review the methods and data sources available for assessing sectoral carbon budgets, and describe some of key data limitations and uncertainties for each sector in different regions of the world. We identify the main gaps in our knowledge/data, show that coverage is better for the developed world for most sectors, and suggest how sectoral carbon budgets could be improved in the future. Research priorities include the development of shared protocols through site networks, a move to full carbon accounting within sectors, and the assessment of full greenhouse gas budgets.

show abstract

Section: Measuring Changes In Carbon In the Soilmentioning

confidence: 99%

Sectoral approaches to improve regional carbon budgets

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sequences amplified from 13 C-labeled DNA or RNA are derived from organisms actively assimilating the substrate. This approach has been used to identify organisms that utilize methane or methanol (4,19), organic compounds (15,20), or CO 2 (6, 9) in microcosms and those that assimilate plant root exudates in the field (28). SIP therefore links phylogeny to ecosystem function and has identified established and novel groups by utilizing labeled compounds in complex soil communities.…”

mentioning

confidence: 99%

Stable Isotope Probing Analysis of Interactions between Ammonia Oxidizers

Tourna

Freitag

Prosser

2010

Appl Environ Microbiol

View full text Add to dashboard Cite

The response of natural microbial communities to environmental change can be assessed by determining DNA-or RNA-targeted changes in relative abundance of 16S rRNA gene sequences by using fingerprinting techniques such as denaturing gradient gel electrophoresis (DNA-DGGE and RNA-DGGE, respectively) or by stable isotope probing (SIP) of 16S rRNA genes following incubation with a 13 C-labeled substrate (DNA-SIP-DGGE). The sensitivities of these three approaches were compared during batch growth of communities containing two or three Nitrosospira pure or enriched cultures with different tolerances to a high ammonia concentration. Cultures were supplied with low, intermediate, or high initial ammonia concentrations and with 13 C-labeled carbon dioxide. DNA-SIP-DGGE provided the most direct evidence for growth and was the most sensitive, with changes in DGGE profiles evident before changes in DNA-and RNA-DGGE profiles and before detectable increases in nitrite and nitrate production. RNA-DGGE provided intermediate sensitivity. In addition, the three molecular methods were used to follow growth of individual strains within communities. In general, changes in relative activities of individual strains within communities could be predicted from monoculture growth characteristics. Ammonia-tolerant Nitrosospira cluster 3b strains dominated mixed communities at all ammonia concentrations, and ammonia-sensitive strains were outcompeted at an intermediate ammonia concentration. However, coexistence of ammonia-tolerant and ammonia-sensitive strains occurred at the lowest ammonia concentration, and, under some conditions, strains inhibited at high ammonia in monoculture were active at high ammonia in mixed cultures, where they coexisted with ammonia-tolerant strains. The results therefore demonstrate the sensitivity of SIP for detection of activity of organisms with relatively low yield and low activity and its ability to follow changes in the structure of interacting microbial communities.

show abstract

“…Secondly, the 13 C contribution was diluted by the newly unlabeled photosynthetically-fixed C as reed growth proceeded (Rangel-Castro et al 2004). Thirdly, the stronger nutrient competitive abilities for reed demand resulted in smaller size of the rhizosphere microbial biomass (Blagodatskaya et al 2010;Kuzyakov and Blagodatskaya 2015) during reed growth, as shown by the reed N uptake under the N-limited condition in our study (Fig.…”

mentioning

confidence: 57%