Carbon fixation into lipid in small freshwater lakes

Wainman, Bruce; Lean, D. R. S.

doi:10.4319/lo.1992.37.5.0956

Cited by 30 publications

(31 citation statements)

References 25 publications

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“…Predicting photosynthate allocation from environmental factors in the NEW, as has previously been attempted in fresh waters (Cuhel & Lean 1987, Lean et al 1989, Wainmann & Lean 1992, does not appear very promising based on the present results. Even the most successful models had R2 values less than 60% and the data often tended towards a discontinuous distribution in the dependent and/or predictor variable (e.g.…”

Section: Discussionmentioning

confidence: 51%

“…Daylength, an important correlate of macromolecular synthesis in some other systems (Cuhel & Lean 1987, Wainmann & Lean 1992, was nominally 24 h throughout the observation period and was treated as a constant.…”

Section: Methodsmentioning

confidence: 99%

“…The same is true of efforts to develop models for predicting the synthesis of macromolecular classes of particular interest, such as lipids (e.g. Wainmann & Lean 1992) and protein (Lean et al 1989). The expedition of RV 'Polarstern' to the Northeast Water in 1993 afforded an opportunity to study biosynthetlc patterns of polar marine phytoplankton in a hydrodynamically complex system.…”

Section: Introductionmentioning

confidence: 98%

“…Of the various macromolecular classes, lipids are of special interest for their nutritional importance to consumers and potential for contaminant transfer (Kattner et al 1989, Wainmann & Lean 1992, Parrish et al 1995. Nutrients and taxonomic affiliation both influence the proportion of fixed carbon that phytoplankton direct to lipids (Shifrin & Chisholm 1981, Madariaga 1992, Fernandez et al 1994a) and there has been some success in predicting lipid biosynthesis from environmental factors in freshwater phytoplankton communltles (Wainmann & Lean 1992).…”

Section: Introductionmentioning

confidence: 99%

“…Nutrients and taxonomic affiliation both influence the proportion of fixed carbon that phytoplankton direct to lipids (Shifrin & Chisholm 1981, Madariaga 1992, Fernandez et al 1994a) and there has been some success in predicting lipid biosynthesis from environmental factors in freshwater phytoplankton communltles (Wainmann & Lean 1992). The total lipid pool comprises several functionally distinct classes of compound (Gurr & Harwood 1991) and previous work with natural marlne phytoplankton has shown that changing allocation to different classes underlies variations in total lipid concentration (Parrish 1987) and synthesis (Smith & D'Souza 1993).…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of macromolecular and lipid classes by phytoplankton in the Northeast Water Polynya

Smith¹,

Gosselin²,

Kattner³

et al. 1997

Mar. Ecol. Prog. Ser.

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The patterns and environmental correlates of macromolecular synthesis were investigated during 1993 in the Northeast Water Polynya, a region of variable ice cover and hydrodynamic regime on the continental shelf of northeast Greenland (77" to 81' N). Allocation of photosynthate to major macromolecular classes (protein, lipid, and polysaccharides), like phytoplankton biomass and primary production, showed strong spatio-temporal variability. Time series observations in the central part of the polynya and correlational analysis over all stations provided physiological evidence of nutrient deficiency in surface populations as nitrate and silicate were depleted. Lipid, rather than polysaccharide, synthes~s was favoured as nutrient concentrations decreased, and C/N assimilation ratios inferred from protein labelling reached very high values ( > l 5 by weight). C/N composition ratios increased in parallel but remained lower (<10) than the assimilation ratios. Lipid synthesis shifted towards neutral lipids and glycolipids, and away from phospholipids, as nutrient concentrations declined to their observed minimum. At stations with a dominance of large (mainly diatom) phytoplankton more of the lipid synthesis was directed to glycolipids and less to phospholipids and neutral lipids than at those dominated by small (mainly flagellate) phytoplankton, but relative allocation to total lipids and other macromolecular classes was not significantly different between the 2 types of stations. Although both nitrate and silicate became depleted, changes in silicate were most closely related to the physiological changes observed, consistent with a predominance of diatoms at the more productive stations.

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Section: Discussionmentioning

confidence: 51%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biosynthesis of macromolecular and lipid classes by phytoplankton in the Northeast Water Polynya

Smith¹,

Gosselin²,

Kattner³

et al. 1997

Mar. Ecol. Prog. Ser.

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Diel Pattern of Photosynthate Partitioning in Phytoplankton Population in an Oligomesotrophic Lake

Maurin¹,

Amblard²,

Bourdier³

1999

International Review of Hydrobiology

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The aim of this study was to measure the short‐term changes in inorganic carbon allocation into various macromolecular compounds (proteins, polysaccharides and lipids) throughout a diurnal cycle in the oligomesotrophic Lake Pavin (Massif Central of France) at the depths of 5, 15 and 30 m. Biochemical fractionation was done by consecutive differential extractions in order to separate proteins, polysaccharides, lipids and low molecular weight compounds (LMW) by virtue of their relative solubilities in different extraction solvents. Over the entire diurnal cycle inorganic carbon was preferentially incorporated into proteins (M = 30%), then into polysaccharides (M = 28%), LMWs (M = 27%) and lipids (M = 15%). However, at 5 m, diurnal variations were reflected by the high percentage of the inorganic carbon incorporated into polysaccharides during periods of high light intensity and decreased at dawn and dusk. The reverse pattern was observed for the allocation of inorganic carbon to proteins.

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