Carbon filament implants promote axonal growth across the transected rat spinal cord

Khan, Talat; Dauzvardis, Michael; Sayers, Scott

doi:10.1016/0006-8993(91)91087-h

Cited by 53 publications

(30 citation statements)

References 17 publications

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“…Scanning confocal laser microscopy indicated that this behaviour seems to have important consequences on the direction of axonal sprouting and regrowth. Intervention strategies intended to promote directed host axonal regeneration across spinal cord transections involve grafting ''scaffolds'' of already highly orientated growthpromoting substrates, such as peripheral nerve, cellular or acellular matrix-bridges, or fine carbon fibre filaments (Richardson et al, 1980;Khan et al, 1991;Paino et al, 1994;Xu et al, 1997). Any axonal regeneration associated with these structures would be capable of following the already determined guidance cues, such as the longitudinally orientated bands of Büngner in transplanted peripheral nerve (Ramon Y Cajal, 1928).…”

Section: Discussionmentioning

confidence: 98%

Spontaneous longitudinally orientated axonal regeneration is associated with the Schwann cell framework within the lesion site following spinal cord compression injury of the rat

et al. 1998

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Spontaneous cellular reorganisation at the lesion site has been investigated following massive spinal cord compression injury in adult rats. By 2 days post operation (p.o.), haemorrhagic necrosis, widespread axonal degeneration, and infiltration by polymorphnuclear granulocytes and OX42-positive macrophages were observed in the lesion site. By 7 days p.o., low affinity nerve growth factor receptor-positive Schwann cells, from activated spinal roots, were identified as they migrated far into the lesion. Between 7 and 14 days p.o., the overlapping processes of Schwann cells within the macrophage-filled lesion formed a glial framework which was associated with extensive longitudinally orientated ingrowth by many neurofilament-positive axons. Relatively few of these axons were calcitonin gene-related peptide (CGRP)-, substance P (SP)-, or serotonin (5HT)-positive; however, many were glycinergic or gamma aminobutyric acid (GABA)ergic. At 21 and 28 days p.o. (the longest survival times studied), a reduced but still substantial amount of orientated Schwann cells and axons could be detected at distances of up to 5 mm within the lesion. Glial fibrillary acidic protein (GFAP) immunoreactivity demonstrated the slow formation of astrocytic scarring which only became apparent at the lesion interface between 21 and 28 days p.o. The current data suggest the possibility of developing future therapeutic strategies designed to maintain or even enhance these spontaneous and orientated regenerative events.

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Section: Discussionmentioning

confidence: 98%

Spontaneous longitudinally orientated axonal regeneration is associated with the Schwann cell framework within the lesion site following spinal cord compression injury of the rat

et al. 1998

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“…Carbon fibers have been shown to be compatible with physiological cells and tissues [14,[20][21][22] and nanodimensioned fibers have excellent conductivity and high strength to weight ratios [23,24]. High conductivity is a promising property as electrical stimulation has been shown to be beneficial for nerve functions and for regeneration [25,26].…”

Section: Introductionmentioning

confidence: 99%

Decreased functions of astrocytes on carbon nanofiber materials

et al. 2004

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“…The studies in vitro and in vivo have shown that CNS axons preferentially elongate along aligned substrates, including adhesion protein and synthetic filament [36,37] . In our experiment, we took L-poly-lysine as matrixes to induce the axon extension.…”

Section: Discussionmentioning

confidence: 99%

Morphological and electrophysiological evidence for regeneration of transected spinal cord fibers and restoration of motor functions in adult rats

Yang

2006

CHINESE SCI BULL

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After 2/3 transection of the right ninth thoracic spinal cord of an adult rat, a chitosan tube seeded with L-poly-lysine was implanted between the rostral and caudal end of the lesioned cord. Twelve months after the operation, regeneration of myelinated and non-myelinated axons and new blood vessels were observed along the wall of the chitosan tube implanted under an electron microscope. Somatosensory evoked potentials (SEP) could be consistently recorded from the left somatosensory cortex following electrical stimulation of the right hind limb, while transcranial magnetic stimulation of the left motor cortex could also evoke motor activity from the right hind limb. The present result suggests that implanted chitosan tube might be useful in regeneration of injured nerve fibers of the spinal cord resulting in a long-term restoration of motor functions.

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Carbon filament implants promote axonal growth across the transected rat spinal cord

Cited by 53 publications

References 17 publications

Spontaneous longitudinally orientated axonal regeneration is associated with the Schwann cell framework within the lesion site following spinal cord compression injury of the rat

Spontaneous longitudinally orientated axonal regeneration is associated with the Schwann cell framework within the lesion site following spinal cord compression injury of the rat

Decreased functions of astrocytes on carbon nanofiber materials

Morphological and electrophysiological evidence for regeneration of transected spinal cord fibers and restoration of motor functions in adult rats

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