Carbon Dots and Fluorescein: The Ideal FRET Pair for the Fabrication of a Precise and Fully Reversible Ammonia Sensor

Abstract

Abstract:Monitoring of ammonia in the human breath is of paramount importance to monitor diseases link to liver and kidney mulfunctioning. The present paper describes a solid-state optical ammonia sensor based on Förster resonance energy transfer (FRET) between narrowly dispersed blue-emitting carbon nanodots (CNDs) as FRET donor and fluorescein as FRET acceptor. The fluorophores were physically entrapped in a close to superhydrophobic sol-gel matrix, in turn deposited on a PVDF-HFP electrospun fiber membrane. The sensor shows a linear calibration with a remarkably low limit of detection, i.e., 110 ppb, and adequate reproducibility up to six N2/NH3 cycles.

Carbon dots and fluorescein: The ideal FRET pair for the fabrication of a precise and fully reversible ammonia sensor

“…Details on the synthetic methods to prepare the single components for the solid-state optical ammonia sensors are described elsewhere in a recently submitted publication [7].…”

Current Advancements in Transdermal Biosensing and Targeted Drug Delivery

“…Quantum dots are semiconductor nanocrystals in the size range of 0.2 to 100 nm (crystals in the size range of 2 to 10 nm are typically used). Due to the small size of these nanoparticles, they exhibit discrete energy levels with the behavior of the electrons and holes in their orbitals governed by quantum mechanics; as a result, small changes in their size (which can be controlled by fabrication techniques) cause changes in their fluorescence properties [ 30 , 31 , 32 ]. These nanostructures have tunable fluorescence properties by controlling their size, material composition, and fabrication temperature; consequently, they have the ability to outperform organic fluorophores due to their strong signal, tunable emission wavelengths, narrow emission profiles, and potential for use with only one light source for excitation for a variety of emission signals.…”

“…These nanostructures have tunable fluorescence properties by controlling their size, material composition, and fabrication temperature; consequently, they have the ability to outperform organic fluorophores due to their strong signal, tunable emission wavelengths, narrow emission profiles, and potential for use with only one light source for excitation for a variety of emission signals. While these nanocrystals have gained attention for a variety of biomedical applications (e.g., in vivo imaging, drug delivery systems), they also have applications in sensing technology [ 31 ]. The excellent optical properties of QDs have allowed them to function in various FRET assays for the detection of biologically relevant molecules (e.g., nucleic acids, proteins, and antibodies).…”

Melt-Spun Photoluminescent Polymer Optical Fibers for Color-Tunable Textile Illumination

“…In this work, we focus on the optical performance of yarns and textiles produced from PL-POF variants containing two different luminescent dyes (multicolor textiles). The subject of interest here is a nonresonant fiber-to-fiber light transfer that is different from a resonant energy transfer [ 26 , 27 , 28 ]. Fiber-to-fiber transfer is based purely on the re-adsorption of escaping light.…”