Carbon dot-DNA-protoporphyrin hybrid hydrogel for sustained photoinduced antimicrobial activity

Kumari, Sonam; Prasad, Surendra Rajit; Mandal, Debabrata; Das, Prolay

doi:10.1016/j.jcis.2019.06.034

Cited by 51 publications

(66 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In hybrid systems, added complexity further necessitates careful consideration of potential undesirable interplay between the carbon dot photosensitization effects and whatever agent(s) accompany it in the system. For example, ROS from carbon dots have been reported to cause degradation of dyes as mentioned previously [ 22 , 49 , 59 ]. An example of this observation is shown in Figure 11 a,b, in which methyl orange (MO) is significantly degraded by photoactivated carbon dots [ 49 ].…”

Section: Carbon Nanodots In Hybrid Photodynamic Systemsmentioning

confidence: 95%

“…These may have been conducted for carbon dots either in an in situ or solution-based context, whereby bacterial cells do or do not play a role in the experimental detection respectively. For intracellular general ROS detection, ROS/RNS assay kits may be used [ 58 ], and/or fluorescence-on probes such as dihydrorhodamine 123 (DHR 123) [ 3 , 16 , 59 ] or dichlorofluorescein-diacetate (DCFH-DA) [ 25 ]. In cell-less, solution-based detection, DCFH can also be used, although without the diacetate [ 19 ].…”

Section: Carbon Nanodots As Photodynamic Antimicrobial Agentsmentioning

confidence: 99%

“…While APDT effects have been reported from carbon dots in a handful of recently emerging studies, their other properties, namely photophysical properties, have conferred advantages for incorporation in photoactivatable antibacterial systems even when no intrinsic APDT characteristics are detected for the carbon dots alone. Studies have capitalized on their visible light absorption characteristics [ 40 ], broad-spectrum absorption [ 59 ], excitation-tunable fluorescence [ 91 ], and up-conversion luminescence [ 12 ] properties to name a few. Carbon dots have, for example, been employed as FRET donor agents for varied applications.…”

Section: Carbon Nanodots In Hybrid Photodynamic Systemsmentioning

confidence: 99%

See 2 more Smart Citations

Carbon Nanodots in Photodynamic Antimicrobial Therapy: A Review

Knoblauch

Geddes

2020

Materials

View full text Add to dashboard Cite

Antibiotic resistance development in bacteria is an ever-increasing global health concern as new resistant strains and/or resistance mechanisms emerge each day, out-pacing the discovery of novel antibiotics. Increasingly, research focuses on alternate techniques, such as antimicrobial photodynamic therapy (APDT) or photocatalytic disinfection, to combat pathogens even before infection occurs. Small molecule “photosensitizers” have been developed to date for this application, using light energy to inflict damage and death on nearby pathogens via the generation of reactive oxygen species (ROS). These molecular agents are frequently limited in widespread application by synthetic expense and complexity. Carbon dots, or fluorescent, quasi-spherical nanoparticle structures, provide an inexpensive and “green” solution for a new class of APDT photosensitizers. To date, reviews have examined the overall antimicrobial properties of carbon dot structures. Herein we provide a focused review on the recent progress for carbon nanodots in photodynamic disinfection, highlighting select studies of carbon dots as intrinsic photosensitizers, structural tuning strategies for optimization, and their use in hybrid disinfection systems and materials. Limitations and challenges are also discussed, and contemporary experimental strategies presented. This review provides a focused foundation for which APDT using carbon dots may be expanded in future research, ultimately on a global scale.

show abstract

Section: Carbon Nanodots In Hybrid Photodynamic Systemsmentioning

confidence: 95%

Section: Carbon Nanodots As Photodynamic Antimicrobial Agentsmentioning

confidence: 99%

Section: Carbon Nanodots In Hybrid Photodynamic Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Carbon Nanodots in Photodynamic Antimicrobial Therapy: A Review

Knoblauch

Geddes

2020

Materials

View full text Add to dashboard Cite

show abstract

“…In addition to the abovementioned non-covalent loading approaches, Kumari and colleagues developed a hybrid hydrogel system using cytosine rich ssDNA covalently attached to carbon dot (CD) and protoporphyrin IX (PpIX) [139]. The CD conjugate acted as a crosslinker and energy donor to excite the antimicrobial photosensitizer.…”

Section: Nucleic Acid Hybrids To Control or Sustain Delivery Of Antimmentioning

confidence: 99%

“…Additionally, in vitro growth assays demonstrated slowed bacteria growth, which was more pronounced than with the free lysozyme ( Figure 8B), and highlights the capacity of origami hybrid systems to also achieve effective antibacterial effects. (CD) and protoporphyrin IX (PpIX) [139]. The CD conjugate acted as a crosslinker and energy donor to excite the antimicrobial photosensitizer.…”

Section: Nucleic Acid Hybrid With Enhanced Binding To Bacteria Cellsmentioning

confidence: 99%

Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers

Obuobi

Škalko‐Basnet

2020

Pharmaceutics

View full text Add to dashboard Cite

Conventional antibiotic therapy is often challenged by poor drug penetration/accumulation at infection sites and poses a significant burden to public health. Effective strategies to enhance the therapeutic efficacy of our existing arsenal include the use of nanoparticulate delivery platforms to improve drug targeting and minimize adverse effects. However, these nanocarriers are often challenged by poor loading efficiency, rapid release and inefficient targeting. Nucleic acid hybrid nanocarriers are nucleic acid nanosystems complexed or functionalized with organic or inorganic materials. Despite their immense potential in antimicrobial therapy, they are seldom utilized against pathogenic bacteria. With the emergence of antimicrobial resistance and the associated complex interplay of factors involved in antibiotic resistance, nucleic acid hybrids represent a unique opportunity to deliver antimicrobials against resistant pathogens and to target specific genes that control virulence or resistance. This review provides an unbiased overview on fabricating strategies for nucleic acid hybrids and addresses the challenges of pristine oligonucleotide nanocarriers. We report recent applications to enhance pathogen targeting, binding and control drug release. As multifunctional next-generational antimicrobials, the challenges and prospect of these nanocarriers are included.

show abstract

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

et al. 2021

View full text Add to dashboard Cite

Nucleic acid-based hydrogels that integrate intrinsic biological properties of nucleic acids and mechanical behavior of their advanced assemblies are appealing bioanalysis and biomedical studies for the development of new-generation smart biomaterials. It is inseparable from development and incorporation of novel structural and functional units. This review highlights different functional units of nucleic acids, polymers, and novel nanomaterials in the order of structures, properties, and functions, and their assembly strategies for the fabrication of nucleic acid-based hydrogels. Also, recent advances in the design of multifunctional and stimuli-responsive nucleic acid-based hydrogels in bioanalysis and biomedical science are discussed, focusing on the applications of customized hydrogels for emerging directions, including 3D cell cultivation and 3D bioprinting. Finally, the key challenge and future perspectives are outlined.

show abstract

Carbon dot-DNA-protoporphyrin hybrid hydrogel for sustained photoinduced antimicrobial activity

Cited by 51 publications

References 46 publications

Carbon Nanodots in Photodynamic Antimicrobial Therapy: A Review

Carbon Nanodots in Photodynamic Antimicrobial Therapy: A Review

Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

Contact Info

Product

Resources

About