Carbon-dot-based ratiometric fluorescent sensor for detecting hydrogen sulfide in aqueous media and inside live cells

Yu, Changmin; Li, Xizhen; Zeng, Fang; Zheng, Fangyuan; Wu, Shuizhu

doi:10.1039/c2cc37329g

Cited by 429 publications

(210 citation statements)

References 37 publications

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“…Although the indirectly measured sulphide concentrations may deviate more or less from the true values due to some systematic errors, the magnitudes as well as the trends of the calculated values are nevertheless reliable. Most previous reports regarding fluorescent sulphide probes were only able to provide static whole-cell images and reveal qualitative fluorescence intensity differences (among these images) after 15-30 min external stimulations with various sulphide concentrations in the mM range 24,26,28,29 To our knowledge, ours is not only the first demonstration of live cell sulphide imaging with nM sensitivity but is also the first time that local variations in intracellular sulphide levels have been tracked dynamically.…”

Section: Resultsmentioning

confidence: 89%

“…[23][24][25][26][27][28]. Although most publications suggest that the average endogenous H 2 S level is in the mM range, much lower sulphide concentrations have been reported 29 . More importantly, the anabolism and catabolism of cellular sulphide are known to be rapid, which means that the sulphide concentration could fluctuate continuously from as high as submM during its explosive production to as low as a few nM after its rapid consumption 30 .…”

Section: Doi: 101038/ncomms2722mentioning

confidence: 99%

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Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

et al. 2013

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Hydrogen sulphide (H 2 S) is a gaseous signalling agent that has important regulatory roles in many biological systems but remains difficult to measure in living biological specimens. Here we introduce a new method for highly sensitive sulphide mapping in live cells via singleparticle plasmonic spectral imaging that uses Au-Ag core-shell nanoparticles as probes. This strategy is based on Ag 2 S formation-induced spectral shifts of the nanoprobes, which is not only highly selective towards sulphide but also shows a linear logarithmic dependence on sulphide concentrations from 0.01 nM to 10 mM. A theoretical model was established that successfully explained the experimental observations, suggesting that the local sulphide concentration as well as its oscillations can be determined indirectly from kinetic measurements of the spectral shifts of the nanoprobes. We demonstrated for the first time the realtime mapping of local variations of sulphide levels in live cells with nM sensitivity.

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Section: Resultsmentioning

confidence: 89%

Section: Doi: 101038/ncomms2722mentioning

confidence: 99%

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

et al. 2013

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“…28 FRET is the interaction between two excited state fluorophores correlated with distance. The process involves the nonradiative transfer of excitation energy from an excited donor to a proximal ground-state acceptor, and it is convenient in the design of ratiometric probes involving the ratio of two emission intensities at different wavelengths.…”

Section: Fluorescent Probes Based On Reducing Azides To Aminesmentioning

confidence: 99%

Fluorescent probes for hydrogen sulfide detection and bioimaging

2014

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In comparison with other biological detection technologies, fluorescence bioimaging technology has become a powerful supporting tool for intracellular detection, and can provide attractive facilities for investigating physiological and pathological processes of interest with high spatial and temporal resolution, less invasiveness, and a rapid response. Due to the versatile roles of hydrogen sulfide (H 2 S) in cellular signal transduction and intracellular redox status regulation, fluorescent probes for the detection of this third signalling gasotransmitter have rapidly increased in number in recent years. These probes can offer powerful means to investigate the physiological actions of H 2 S in its native environments without disturbing its endogenous distribution. In this feature article, we address the synthesis and design strategies for the development of fluorescent probes for H 2 S based on the reaction type between H 2 S and the probes. Moreover, we also highlight fluorescent probes for other reactive sulfur species, such as sulfane sulfurs and SO 2 derivatives.

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“…Yu et al [87] reported a naphthalimide azide anchored CDs for the selective sensing for H 2 S. It is a fluorescence resonance energy transfer (FRET)-based ratiometric sensor which can ensure more accurate detection with a detection limit of 10 nM.…”

Section: Organic/biological Molecular and Target Gas Detectionmentioning

confidence: 99%

Controllable Synthesis of Fluorescent Carbon Dots and Their Detection Application as Nanoprobes

Yang

et al. 2013

Nano-Micro Lett.

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Abstract:Carbon dots (CDs), as a new member of carbon nanomaterial family, have aroused great interest since their discovery in 2004. Because of their outstanding water solubility, high sensitivity and selectivity to target analytes, low toxicity, favorable biocompatibility, and excellent photostability, researchers from diverse disciplines have come together to further develop the fundamental properties of CDs. Many methods for the production of CDs have been reported, therein, hydrothermal and solvothermal technology needs simple equipments, and microwave synthesis needs less reaction time, hence these methods become current common synthesis methods, in which many precursors have been applied to produce CDs. Due to their excellent fluorescence, CDs have made impressive strides in sensitivity and selectivity to a diverse array of salt ions, organic/biological molecules and target gases. The development of CDs as nanoprobes is still in its infancy, but continued progress may lead to their integration into environmental and biological applications. Hydrothermal, solvothermal, and microwave synthesis of fluorescent carbon dots and their detection applications as nanoprobes in salt ions, organic/biological molecules, and target gases will be reviewed.

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Carbon-dot-based ratiometric fluorescent sensor for detecting hydrogen sulfide in aqueous media and inside live cells

Cited by 429 publications

References 37 publications

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

Highly sensitive sulphide mapping in live cells by kinetic spectral analysis of single Au-Ag core-shell nanoparticles

Fluorescent probes for hydrogen sulfide detection and bioimaging

Controllable Synthesis of Fluorescent Carbon Dots and Their Detection Application as Nanoprobes

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