Carbon Dioxide, Oxygen and Ethylene Effects on Potato Tuber Dormancy Release and Sprout Growth

Coleman, Warren K.

doi:10.1006/anbo.1998.0645

Cited by 37 publications

(26 citation statements)

References 34 publications

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“…Particularly effective in dormancy breaking was ethylene chlorhydrin, which resulted in 50% of tubers sprouting within 4 days of treatment, and 100% after 20 days. Similar results were obtained by Coleman (1998), who also showed that exogenous ethylene caused a reduction in ABA content. Ethylene is thought to play a critical role in the induction of tuber dormancy but was also reported to increase during onset of sprouting (Suttle 2007).…”

Section: Discussionsupporting

confidence: 78%

Effect of Growth Regulators and Ethanol on Termination of Dormancy in Potato Tubers

2017

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The main objective of this study was to find the best practice of inducing the sprouting of dormant potato tubers. We compared two protocols of breakage of dormancy, which are based on dipping excised potato eyes in an aqueous solution of gibberellic acid (GA 3 ) and kinetin (standard 1) or in the aqueous solution of GA 3 , thiourea, and daminozide (standard 2), with a newly reported approach based on ethanol. We tested the effect of ethanol alone or in combination with GA 3 and/or kinetin on dormancy release and sprouting of the potato tubers. As a model, we used two potato genotypes (cultivars Pasat and Dorota), with long dormancy of 5 and 10 weeks respectively. We showed that the standard 2 was the most effective treatment both for dormancy breaking and in promoting sprout growth, especially for cv. Dorota, for which the treatment induced 82.3% of tuber eye-plugs to sprout 28 days after treatment and to produce 93.2% of emerged plants after subsequent 28 days of cultivation in the greenhouse. For this cultivar, similar efficacy was observed for the combination of 4% ethanol with GA 3 and kinetin. The same concentration of ethanol combined with GA 3 but without kinetin was the most efficient treatment for breaking dormancy of cultivar Pasat. However, the difference between the various treatment combinations was statistically insignificant. Ethanol alone or in combination with kinetin poorly induced breakage of dormancy, confirming the main role of GA 3 in artificial dormancy breaking. Thus our study showed that the standard 2 is the most effective approach for breakage of dormancy at least with long term-dormancy cultivars.Resumen El principal objetivo de este estudio fue encontrar la mejor práctica para inducir la brotación en tubérculos de papa en dormancia. Comparamos dos protocolos para romper la dormancia, basados en inmersión de yemas (ojos) aislados del tubérculo, en una solución acuosa de ácido giberélico (GA3) y cinetina (estándar 1), o en una solución acuosa de GA3, tiourea, y daminozida (estándar 2), con un nuevo enfoque reportado basado en etanol. Probamos el efecto del etanol solo o en combinación con GA3 y/o cinetina en la liberación de la dormancia y en la brotación de los tubérculos de papa. Como modelo usamos dos genotipos de papa (variedades Pasat y Dorota), con dormancia larga de 5 y 10 semanas, respectivamente. Mostramos que el estándar 2 fue el tratamiento más efectivo tanto para romper la dormancia como para promover el crecimiento del brote, especialmente para la variedad Dorota, para la cual el tratamiento indujo un 82.3% de los ojos del tubérculo a brotar 28 días después del tratamiento, y a producir 93.2% de plantas emergidas después de 28 días subsecuentes de cultivo en invernadero. Para esta variedad se observó eficacia similar con la combinación de 4% de etanol con GA3 y cinetina. La misma concentración de etanol en combinación con GA3, pero sin cinetina, fue el tratamiento más eficiente para romper la dormancia de la variedad Pasat. No obstante, la diferencia entre las div...

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Section: Discussionsupporting

confidence: 78%

Effect of Growth Regulators and Ethanol on Termination of Dormancy in Potato Tubers

2017

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“…Potato tubers can synthesize (Evensen 1984) and respond to ethylene (Janes and Wiest 1980, Coleman 1998, Prange et al. 1998), and have been shown to accumulate ethylene during ageing (Mikitzel and Knowles 1989b).…”

Section: Introductionmentioning

confidence: 99%

“…Potato tubers can synthesize (Evensen 1984) and respond to ethylene (Janes and Wiest 1980, Coleman 1998, Prange et al 1998, and have been shown to accumulate ethylene during ageing (Mikitzel and Knowles 1989b). Because PAL can be induced by ethylene, older tubers may thus have a greater potential to sustain higher rates of PAL activity during healing than younger tubers.…”

Section: Introductionmentioning

confidence: 99%

Wound‐induced superoxide production and PAL activity decline with potato tuber age and wound healing ability

Kumar

Knowles²

2003

Physiologia Plantarum

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Wound healing of potato tubers involves the concerted action of several enzymes that facilitate polymerization of phenolics into suberin at the wound site. A decline in the efficiency of healing and resistance to pathogens with advancing tuber age was associated with reduced ability of older tubers to produce superoxide radicals (FRs) in response to wounding. Autophotographs of luminol‐treated longitudinal sections of tissue from 6‐, 18‐ and 30‐month‐old tubers revealed a substantial decline in superoxide production at the wound surface with advancing age. Older tubers were less able to respond to wounding by increasing phenylalanine ammonia lyase (PAL) activity. This enzyme produces t‐cinnamic acid, which constitutes a component of the phenolic domain of suberin, and is normally induced by wounding and/or ethylene. Interestingly, the ability of wounded tissue to oxidize exogenous 1‐aminocyclopropane‐1‐carboxylic acid (ACC) to C2H4 also decreased with advancing tuber age. The oxidation of ACC was inhibited by the FR scavenger, n‐propyl gallate (PG), and inhibition was greatest in tissue from younger tubers, reflecting their greater ability to produce superoxide radicals upon wounding. Regardless of tuber age, 1‐aminocyclobutane‐1‐carboxylic acid, an ACC oxidase inhibitor, did not inhibit C2H4 generation from exogenous ACC. Hence, C2H4 production from ACC by wounded tuber tissue is largely non‐enzymatic and FR‐driven, and thus serves as an indicator of the ability of wounded tissue to produce superoxide. Age‐induced reduction in PAL activity and FR production at the wound surface probably limited the oxidative polymerization of phenolics into suberin during wound periderm formation. The age‐induced loss in ability of wounded tissue to heal and resist pathogens is thus consistent with reduced synthesis and polymerization of phenolic adducts into suberin, a consequence of reduced FR and PAL activity at the wound surface.

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“…4 Sharma et al 15 reported an increase in the starch content and a reduction in the sucrose content of tubers from plants grown from seed tubers that had received five foliar applications of CCC, suggesting changes in metabolic activity, which were not observed, however, in tubers from TPS. 13 Although CCC and daminozide did not affect sprout number, irrespective of the mode of application, once sprouts had achieved a length of 2 mm (used as the criterion for sprouting 20,23 ), subsequent elongation was slower than in the control (data not shown).…”

Section: Resultsmentioning

confidence: 93%

The effect of the time and mode of application of gibberellic acid and inhibitors of gibberellin biosynthesis on the dormancy of potato tubers grown from true potato seed

et al. 2007

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Gibberellic acid (GA 3 ) and inhibitors of gibberellin biosynthesis (daminozide and chlormequat chloride) were applied to the foliage of potato plants grown from true seed (TPS) either once at two stages of plant development, 40 and 60 days after transplantation (DAT), or repeatedly at 10 day intervals starting from the same growth stages. When GA 3 was applied towards the end of the vegetative cycle (either singly 60 DAT or repeatedly from the same date), it induced rapid breakage of tuber dormancy, a reduction in specific weight, a higher rate of respiration and increased weight loss during storage. Single applications of GA 3 early in the vegetative cycle (40 DAT) had no effect on the weight loss and specific weight of tubers during storage, whereas repeated foliar applications of GA 3 starting from the same stage resulted in the formation of tubers with a low specific weight and a high rate of weight loss during storage. However, the tubers from these treatments did not break dormancy uniformly, and, although in the early stages of storage they exhibited a high rate of respiration, this declined to the level of the control (no growth regulator applied). Although daminozide and chlormequat chloride did not affect the duration of tuber dormancy and had little or no effect on any of the other metabolic indicators studied, gibberellin is nevertheless implicated in dormancy breakage, and its application late in the growth cycle may be of practical value in cases where tubers are required for planting soon after harvest. Overall, tubers from TPS respond to plant growth regulator treatment in a similar way to those from plants grown from seed tubers.

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Carbon Dioxide, Oxygen and Ethylene Effects on Potato Tuber Dormancy Release and Sprout Growth

Cited by 37 publications

References 34 publications

Effect of Growth Regulators and Ethanol on Termination of Dormancy in Potato Tubers

Effect of Growth Regulators and Ethanol on Termination of Dormancy in Potato Tubers

Wound‐induced superoxide production and PAL activity decline with potato tuber age and wound healing ability

The effect of the time and mode of application of gibberellic acid and inhibitors of gibberellin biosynthesis on the dormancy of potato tubers grown from true potato seed

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