Carbon Dioxide Enrichment during Photoautotrophic Micropropagation of Protea cynaroides L. Plantlets Improves In Vitro Growth, Net Photosynthetic Rate, and Acclimatization

Abstract

<jats:p>The effects of CO<jats:sub>2</jats:sub> enrichment on the in vitro growth and acclimatization of <jats:italic>Protea cynaroides</jats:italic> L. plantlets were investigated. Three CO<jats:sub>2</jats:sub> enrichment concentrations were used: 0 (control), 1000, 5000, and 10000 μmol·mol<jats:sup>−1</jats:sup>. Plantlets in the control treatment were cultured on half-strength Murashige and Skoog (MS) medium supplemented with sucrose, whereas those enriched with different CO<jats:sub>2</jats:sub> concentrations were grown on sucrose-free MS medium. Compared with the control, significant improvements were observed in the growth of plantlets enriched with CO<jats:sub>2</jats:sub> irrespective of the concentration. Plantlets enriched with 5000 μmol·mol<jats:sup>−1</jats:sup> CO<jats:sub>2</jats:sub> produced the highest number of leaves and the largest leaf area. In addition, the photosynthetic ability of plantlets enriched with CO<jats:sub>2</jats:sub> was enhanced, which resulted in significant increases in shoot growth and dry matter accumulation. In particular, the shoot dry weight of plantlets cultured in 5000 μmol·mol<jats:sup>−1</jats:sup> CO<jats:sub>2</jats:sub> and 10000 μmol·mol<jats:sup>−1</jats:sup> CO<jats:sub>2</jats:sub> were, respectively, 2.1 and 4.2 times higher than those without CO<jats:sub>2</jats:sub> enrichment. During acclimatization, the survival percentage, rooting percentage, and leaf number of plantlets grown in elevated CO<jats:sub>2</jats:sub> were, respectively, up to 4.5, 1.8, and 2.7 times higher than plantlets without CO<jats:sub>2</jats:sub> enrichment. The improvements in survival percentage and ex vitro growth of these plantlets were the result of their enhanced photosynthetic ability in vitro, which resulted in the production of high-quality plantlets. Significant improvements in the overall growth of <jats:italic>P. cynaroides</jats:italic> plantlets were achieved through the use of photoautotrophic micropropagation with CO<jats:sub>2</jats:sub> enrichment.</jats:p>

Interaction of Supplementary Light and CO2 Enrichment Improves Growth, Photosynthesis, Yield, and Quality of Tomato in Autumn through Spring Greenhouse Production

“…We found CeLn, CeLn, and CeLs increased dry weight accumulation in shoots and roots, which agrees with the result of Fierro et al (1994), but they also shifted dry weight to the roots, resulting in a higher root-toshoot ratio compared with CaLn. This result is consistent with the effects of CO 2 enrichment in grape (Wu and Lin, 2013) and in Rhodes grass (Ksiksi and Youssef, 2010), indicating supplementary light and CO 2 enrichment may promote the uptake of nutrients from culture substrate by altering the distribution of dry weight and thus increasing yields.…”

Analysis effect of shade level on the physiological and anatomical characteristics of hybrid Phalaenopsis orchid at the acclimatization stage

“…However, there was an increase compared to the fresh weight at the beginning of the study. Fresh weight was the accumulation of photosynthate and water content (Wu and Lin, 2013). The sunlight, water, and nutrients affected the photosynthate and water content (Febriyono et al, 2017).…”

Naungan dan Tipe Substrat Berbeda pada Periode Aklimatisasi Ex-vitro Phalaenopsis Hibrid

“…Sementara kelangsungan hidup plantlet selama 5 minggu ditunjukkan oleh keberhasilan hidup 100% pada semua tipe substrat. Berat segar plantlet merupakan akumulasi fotosintat dan kandungan air (Wu dan Lin, 2013)…”