Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells

Reismann, Marc; Lehmann, Daniel; Quandte, Matthias; Ure, Benno M.; Glüer, S.

doi:10.1007/s00383-008-2274-x

Cited by 5 publications

(2 citation statements)

References 19 publications

(32 reference statements)

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“…Hypoxia in particular, a known inductor of dediff erentiation which is correlated to a poor outcome in neuroblastoma, might contribute to this eff ect [7] . In previous studies we were unable to explain these observations in terms of cell proliferation [22] and, recently, DNA methylation [19] under in vitro conditions. In this study we investigated a third level, possibly explaining the higher metastatic rates of neuroblastoma cells after conditions mimicking pneumoperitoneum: the expression of promalignant genes.…”

Section: Discussionmentioning

confidence: 72%

Carbon dioxide, Hypoxia and Low pH Lead to Overexpression ofc-mycandHMGB-1Oncogenes in Neuroblastoma Cells

et al. 2009

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Section: Discussionmentioning

confidence: 72%

Carbon dioxide, Hypoxia and Low pH Lead to Overexpression ofc-mycandHMGB-1Oncogenes in Neuroblastoma Cells

et al. 2009

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“…The few attempts to investigate CO 2 effects on NB cells have been focused on cell proliferation/migration, epigenetic changes, apoptosis, and expression of High Mobility Group Box (HMGB)-1 and c-MYC. 3,4,16,17 In previous studies, we evaluated alterations in cell redox status induced by CO 2 and its potential impact on human SH-SY5Y NB cells. Our results showed that in vitro simulated CO 2 pneumoperitoneum environment induces oxidative stress, leading to DNA damage followed by a significant attenuation of cell proliferation mediated by p53 activation.…”

Section: Discussionmentioning

confidence: 99%

CO2 Pneumoperitoneum Effects on Molecular Markers of Tumor Invasiveness in SH-SY5Y Neuroblastoma Cells

et al. 2019

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Introduction CO2 pneumoperitoneum can influence the biological behavior of neuroblastoma (NB). Angiogenesis and genetic features are responsible for malignant phenotype of this tumor. We examined the CO2 effects on N-Myc, vascular endothelial growth factor (VEGF), and matrix metalloproteinase-2 (MMP-2) expression as critical biomarkers of tumor invasiveness, in NB cells without N-Myc amplification. Materials and Methods SH-SY5Y cells were exposed to CO2 (100%) at 15 mm Hg pressure for 4 hours and then moved to normal condition for 24 hours. Control cells were incubated with 5% CO2 for the same time. In control and CO2-exposed cells, the messenger ribonucleic acid (mRNA) levels of hypoxia-inducible factor (HIF)-1α, HIF-2α, VEGF-A, and MMP-2 were quantified by real-time polymerase chain reaction. N-Myc expression was evaluated by Western blot analysis. Results The exposure to 15 mm Hg CO2 (100%) for 4 hours induced an increase in HIF-1α, but not in HIF-2α, mRNA levels. No differences were observed in N-Myc expression between exposed and control cells at each incubation time. Similarly, no significant differences were found for VEGF-A and MMP-2 transcript levels. In CO2 exposed cells, we observed only a slight increase in both VEGF-A and MMP-2 mRNA levels after 4 and 24 hours in comparison to controls. Conclusion In our study, the hypoxic environment induced by CO2 exposure does not affect the expression of critical biomarkers of NB aggressiveness, such as N-Myc, VEGF, and MMP-2, in human SH-SY5Y NB cells without N-Myc amplification. These data suggest that CO2 pneumoperitoneum might not adversely impact NB cell invasiveness; however, it is necessary to evaluate these effects in others in vitro and in vivo models.

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In vitro CO2-induced ROS production impairs cell cycle in SH-SY5Y neuroblastoma cells

et al. 2012

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Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells

Abstract: Exposure of neuroblastoma cells to 100% CO(2) does not alter methylation of two prognostic relevant index genes. It seems therefore unlikely that effects on methylation levels within CO(2) pneumoperitoneum lead to epigenetic changes in neuroblastoma.

Cited by 5 publications

References 19 publications

Carbon dioxide, Hypoxia and Low pH Lead to Overexpression ofc-mycandHMGB-1Oncogenes in Neuroblastoma Cells

Carbon dioxide, Hypoxia and Low pH Lead to Overexpression ofc-mycandHMGB-1Oncogenes in Neuroblastoma Cells

CO2 Pneumoperitoneum Effects on Molecular Markers of Tumor Invasiveness in SH-SY5Y Neuroblastoma Cells

In vitro CO2-induced ROS production impairs cell cycle in SH-SY5Y neuroblastoma cells

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