Carbon dioxide bioconversion into single cell oils (lipids) in two reactors inoculated with Acetobacterium woodii and Rhodosporidium toruloides

Bioconversion of cellulose into bisabolene using Ruminococcus flavefaciens and Rhodosporidium toruloides

“…In addition, an interaction effect was detected between succinic acid and acetic acid concentrations (p = 2.2 × 10 -4 ). This was expected as previous studies have shown that above organic acid concentrations of around 15-20 g/L, growth is inhibited (Huang et al, 2016;Robles-Iglesias et al, 2021). The effect of succinic acid concentration was also found to be non-linear (p = 6.6 × 10 -4 ).…”

“…A definitive screening design (DSD) was selected, as this type of screening design was found to be extremely efficient and informative for the optimisation of isoprenoid production by Saccharomyces cerevisiae (Walls et al, 2022, Walls et al, 2021b The initial concentrations of succinic and acetic acids were deemed critical factors in the study. Previous studies have demonstrated the suitability of acetic acid as a carbon source for lipid (Huang et al, 2016;Robles-Iglesias et al, 2021) and bisabolene production in the species (Magurudeniya et al, 2021;Sundstrom et al, 2018). However, previous studies found that at concentrations above ~ 16 g/L acetic acid had a significant inhibitory effect resulting in an extended lag phase and restricted growth (Robles-Iglesias et al, 2021).…”

“…Previous studies have demonstrated the suitability of acetic acid as a carbon source for lipid (Huang et al, 2016;Robles-Iglesias et al, 2021) and bisabolene production in the species (Magurudeniya et al, 2021;Sundstrom et al, 2018). However, previous studies found that at concentrations above ~ 16 g/L acetic acid had a significant inhibitory effect resulting in an extended lag phase and restricted growth (Robles-Iglesias et al, 2021). As tolerance to succinic acid and the suitability of these organic acids for heterologous isoprenoid biosynthesis have been less extensively characterised, these were a focus of the preliminary characterisation study.…”

Toward sustainable jet fuels: bioconversion of cellulose into isoprenoid biojet candidates using rumen bacteria and non-conventional yeast

“…A definitive screening design (DSD) was selected, as this type of screening design was found to be extremely efficient and informative for the optimisation of isoprenoid production by Saccharomyces cerevisiae (Walls et al, 2022, 2021b) The initial concentrations of succinic and acetic acids were deemed critical factors in the study. Previous studies have demonstrated the suitability of acetic acid as a carbon source for lipid (Huang et al, 2016; Robles-Iglesias et al, 2021) and bisabolene production in the species (Magurudeniya et al, 2021; Sundstrom et al, 2018). However, previous studies found that at concentrations above ∼16 g/L acetic acid had a significant inhibitory effect resulting in an extended lag phase and restricted growth (Robles-Iglesias et al, 2021).…”

“…Previous studies have demonstrated the suitability of acetic acid as a carbon source for lipid (Huang et al, 2016;Robles-Iglesias et al, 2021) and bisabolene production in the species (Magurudeniya et al, 2021;Sundstrom et al, 2018). However, previous studies found that at concentrations above ~16 g/L acetic acid had a significant inhibitory effect resulting in an extended lag phase and restricted growth (Robles-Iglesias et al, 2021). As tolerance to succinic acid and the suitability of these organic acids for heterologous isoprenoid biosynthesis have been less extensively characterised, these were a 14 focus of the preliminary characterisation study.…”