Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids

Prášek, Jan; Húska, Dalibor; Jašek, Ondřej; Zajı́čková, Lenka; Trnková, Libuše; Adam, Vojtěch; Kizek, René; Hubálek, Jaromír

doi:10.1186/1556-276x-6-385

Cited by 16 publications

(6 citation statements)

References 18 publications

(17 reference statements)

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“…The working carbon electrodes based on composite materials can be used as voltammetric sensors for the determination of inorganic analytes 3,24, as well as organic compounds 6, 25. Nowadays, the development of the methods for the determination of carbohydrates 7, biometabolites 13 or anticancer drugs 26 is on the rise.…”

Section: Introductionmentioning

confidence: 99%

Defect‐Rich CoP/Nitrogen‐Doped Carbon Composites Derived from a Metal–Organic Framework: High‐Performance Electrocatalysts for the Hydrogen Evolution Reaction

et al. 2015

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A defect‐rich CoP/nitrogen‐doped carbon composite is reported for the first time derived from ZIF‐67 by means of low‐temperature phosphidation process. As a hydrogen evolution reaction electrocatalyst, the obtained CoP‐N‐C has high HER activity and good stability with a low onset overpotential of 31 mV, a small Tafel slope of 42 mV dec−1, a large exchange current density of 1.6×10−1 mA cm−2, and a 10 mA cm−2 current density at overpotential 91 mV.

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Section: Introductionmentioning

confidence: 99%

Defect‐Rich CoP/Nitrogen‐Doped Carbon Composites Derived from a Metal–Organic Framework: High‐Performance Electrocatalysts for the Hydrogen Evolution Reaction

et al. 2015

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“…For each ODN, calibration curves were determined within the concentration range from 50 ng/mL to 25 μg/mL. The applied amount of ODN, quantitatively determined using UV/Vis spectrometry [ 20 , 55 – 57 ], (data is not shown) served as a control. The influence of ODN concentration on peak A and B height was determined.…”

Section: Resultsmentioning

confidence: 99%

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

et al. 2014

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The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected—peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.

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“…Cytosine-adenine reduction (CA peak) was detected by square wave voltammetry coupled with adsorptive transfer technique (AdTS SWV) [45,46,47,48,49,50]. The parameters of the electrochemical determination were as it follows: initial potential 0 V; end potential –1.85 V; frequency 80 Hz; potential step 0.005 V; amplitude 0.025 V. Time of accumulation was optimized.…”

Section: Methodsmentioning

confidence: 99%

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

Krejčová

Hynek

Kopel

et al. 2013

Viruses

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Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads® with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids.

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Carbon composite micro- and nano-tubes-based electrodes for detection of nucleic acids

Cited by 16 publications

References 18 publications

Defect‐Rich CoP/Nitrogen‐Doped Carbon Composites Derived from a Metal–Organic Framework: High‐Performance Electrocatalysts for the Hydrogen Evolution Reaction

Defect‐Rich CoP/Nitrogen‐Doped Carbon Composites Derived from a Metal–Organic Framework: High‐Performance Electrocatalysts for the Hydrogen Evolution Reaction

Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Development of a Magnetic Electrochemical Bar Code Array for Point Mutation Detection in the H5N1 Neuraminidase Gene

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