Carbon competition between fatty acids and starch during benzoin seeds maturation slows oil accumulation speed

Zhang, Zihan; Wang, Xiaojun; Luo, Ying; Yu, Fangyuan

doi:10.1007/s00468-017-1528-4

Cited by 18 publications

(53 citation statements)

References 36 publications

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“…Based on the observed changes in oil content and quality reported in Fig. 1, and related changes in morphological and physiological indicators in previous studies [15,17], we defined four stages of kernel development: an initial stage prior to the major rise in content (50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60), an active stage of rising oil content (60-80 DAF), a third stage with decreased oil content and high polyunsaturated FAs (80-120 DAF), and a final maturation stage with stable oil content and composition (120-150 DAF). To explore changes in transcription, we sampled these stages at 50, 70, 100 and 130 DAF.…”

Section: Sequence Analysis and De Novo Transcriptome Assemblymentioning

confidence: 76%

“…Oil accumulation and biodiesel fuel property assessment in developing S. tonkinensis kernels Oil content is a principal factor for evaluating the potential of biodiesel feedstocks [4,9]. Previous studies have shown that the oil content of fully ripened S. tonkinensis kernels ranges from 58.6 to 63.0% [9,14,15], which is higher than many other oil plants including L. glauca (31.6%), P. chinensis (35.1%), J. curcas (39.8%), and Vernicia fordii (Hemsl.) Airy Shaw (40.0%) [32,[35][36][37].…”

Section: Discussionmentioning

confidence: 99%

“…To date, related studies on S. tonkinensis have focused mostly on cultivation, wood production, benzoin resin production, and biofuel production [11][12][13][14]. To study development of S. tonkinensis kernels, Zhang et al [15] collected samples from 30 to 140 days after flowering (DAF) for analyses of morphometric indicators (longitudinal sections, weight and water content), nutritional components (oil, soluble sugar, starch, free amino-acid and soluble protein) and related enzyme activities. The dynamic pattern of oil accumulation in developing S. tonkinensis seed kernels has also been analyzed [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…To study development of S. tonkinensis kernels, Zhang et al [15] collected samples from 30 to 140 days after flowering (DAF) for analyses of morphometric indicators (longitudinal sections, weight and water content), nutritional components (oil, soluble sugar, starch, free amino-acid and soluble protein) and related enzyme activities. The dynamic pattern of oil accumulation in developing S. tonkinensis seed kernels has also been analyzed [15][16][17]. However, little is known about the molecular regulatory mechanism of oil accumulation in this species, and no RNA-seq datasets of any sort are available for any member of the Styracaceae.…”

Section: Introductionmentioning

confidence: 99%

“…Key catalytic enzymes include the pyruvate dehydrogenase complex (PDC), the acetyl-CoA carboxylase (ACC) complex, the fatty acid synthase (FAS) complex, and acyl-CoA:DAG acyltransferase (DGAT) [18][19][20]. Zhang et al [15] have previously shown that PDC and ACC activities support FA biosynthesis in developing S. tonkinensis kernels, and DGAT supports TAG biosynthesis. Different parts of the process are localized in different compartments; for example, glycolysis in the cytosol and plastids, FA biosynthesis in plastids, and TAG biosynthesis in endoplasmic reticulum (ER) [20].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Cao

Chen

et al. 2020

BMC Plant Biol

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Background: Styrax tonkinensis (Pierre) Craib ex Hartwich has great potential as a woody biodiesel species having seed kernels with high oil content, excellent fatty acid composition and good fuel properties. However, no transcriptome information is available on the molecular regulatory mechanism of oil accumulation in developing S. tonkinensis kernels. Results: The dynamic patterns of oil content and fatty acid composition at 11 time points from 50 to 150 days after flowering (DAF) were analyzed. The percent oil content showed an up-down-up pattern, with yield and degree of unsaturation peaking on or after 140 DAF. Four time points (50, 70, 100, and 130 DAF) were selected for Illumina transcriptome sequencing. Approximately 73 million high quality clean reads were generated, and then assembled into 168,207 unigenes with a mean length of 854 bp. There were 5916 genes that were differentially expressed between different time points. These differentially expressed genes were grouped into 9 clusters based on their expression patterns. Expression patterns of a subset of 12 unigenes were confirmed by qRT-PCR. Based on their functional annotation through the Basic Local Alignment Search Tool and publicly available protein databases, specific unigenes encoding key enzymes, transmembrane transporters, and transcription factors associated with oil accumulation were determined. Three main patterns of expression were evident. Most unigenes peaked at 70 DAF, coincident with a rapid increase in oil content during kernel development. Unigenes with high expression at 50 DAF were associated with plastid formation and earlier stages of oil synthesis, including pyruvate and acetyl-CoA formation. Unigenes associated with triacylglycerol biosynthesis and oil body development peaked at 100 or 130 DAF.

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Section: Sequence Analysis and De Novo Transcriptome Assemblymentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Cao

Chen

et al. 2020

BMC Plant Biol

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Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: Styrax tonkinensis is a white-flowered tree with considerable potential as a feedstock source for biodiesel production from the oily seed contained within its nutlike drupes. Transcriptome changes during oil accumulation have been previously reported, but not concurrent changes in the proteome. RESULTS: Using proteomic analysis of samples collected at 50, 70, 100 and 130 days after flowering (DAF), we identified 1472 differentially expressed proteins (DEPs). Based on their expression patterns, we grouped the DEPs into nine clusters and analyzed the pathway enrichment. Proteins related to starch and sucrose metabolism were most abundant at 50 DAF. Proteins involved in fatty acid (FA) biosynthesis were mainly grouped into a cluster that peaked at 70 DAF. Proteins related to protein processing in endoplasmic reticulum had two major patterns, trending either upwards or downwards, while proteins involved in amino acid biosynthesis showed more complex relationships. We identified 42 key enzymes involved in lipid accumulation during kernel development, including the acetyl-CoA carboxylase complex (ACC) and the pyruvate dehydrogenase complex (PDC). One oil body membrane protein, oleosin, continuously increased during kernel development.CONCLUSION: A regulatory network of oil accumulation processes was built based on protein and available transcriptome expression data, which were in good temporal agreement. This analysis placed ACC and PDC in the center of the network, suggesting that the glycolytic provision of substrate plays a central regulatory role in FA biosynthesis and oil accumulation.

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Fruit Spray of 24-Epibrassinolide and Fruit Shade Alter Pericarp Photosynthesis Activity and Seed Lipid Accumulation in Styrax tonkinensis

Zhang

Luo

Wang

et al. 2017

J Plant Growth Regul

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Carbon competition between fatty acids and starch during benzoin seeds maturation slows oil accumulation speed

Cited by 18 publications

References 36 publications

Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Transcriptome analysis of metabolic pathways associated with oil accumulation in developing seed kernels of Styrax tonkinensis, a woody biodiesel species

Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development

Fruit Spray of 24-Epibrassinolide and Fruit Shade Alter Pericarp Photosynthesis Activity and Seed Lipid Accumulation in Styrax tonkinensis

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