Carbon and nitrogen sources of planktonic bacteria in Lake Constance studied by the composition and isotope dilution of intracellular amino acids

Simon, Meinhard; Rosenstock, Bernd

doi:10.4319/lo.1992.37.7.1496

Cited by 42 publications

(80 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The low occurrence of arginine among free amino acids in the four lakes (arginine on average made up 3% of all free amino acids) agrees with amino-acid profiles of other lakes (Jørgensen, 1987;Simon and Rosenstock, 1992). In general, arginine is not a major amino acid in proteins of aquatic organisms (constitutes 3-8% of protein amino acids; compilation from several sources), but arginine may still be an important amino acid to bacteria owing to its high C/N ratio of 3.…”

Section: Discussionsupporting

confidence: 54%

Links between bacterial production, amino-acid utilization and community composition in productive lakes

et al. 2007

View full text Add to dashboard Cite

Influence of distribution and abundance of bacterial taxa on ecosystem function are poorly understood for natural microbial communities. We related 16S rRNA-based terminal restriction fragment length polymorphism to bacterial production and arginine uptake kinetics to test if functional features of bacterioplankton in four lakes could be predicted from community composition. Maximum arginine uptake rate (arginine V max ) ranged from 10% to 100% of bacterial production. Owing to high growth efficiencies on arginine (63-77%), the bacterial community could potentially saturate its carbon demand using this single organic substrate, for example, during sudden surges of free amino acids. However, due to low in situ concentrations of arginine in these lakes (o0.9 lg l À1 ), actual uptake rates at ambient concentrations rarely exceeded 10% of V max . Bacterial production and arginine V max could be predicted from a subset of bacterial ribotypes, tentatively affiliated with several bacterial divisions (Cyanobacteria, Actinobacteria, Bacteroidetes and Proteobacteria). Multivariate statistical analysis indicates that there were both highly important and less important ribotypes for the prediction of bacterial production and arginine V max . These populations were either negatively or positively related to the respective functional feature, indicating contrasting ecological roles. Our study provides a statistically robust demonstration that, apart from environmental conditions, patterns in bacterial community composition can also be used to predict lake ecosystem function.

show abstract

Section: Discussionsupporting

confidence: 54%

Links between bacterial production, amino-acid utilization and community composition in productive lakes

et al. 2007

View full text Add to dashboard Cite

show abstract

“…So, it is possible that, at times, the bio-availability of these compounds could also have been a limiting factor for the development of bacterioplankton. A previous study showed that DFAA and DCAA may contribute substantially to bacterial growth Rosenstock 1992), andChoi et al (1999) recorded a large increase in the percentage of CTC-positive bacteria with the addition of mixed amino acids. Moreover, Rosenstock & Simon (1993) reported an increased use of DCAA by bacterioplankton, when these components were resistant to the microbial degradation.…”

Section: Relations With the Other Parameters Measuredmentioning

confidence: 99%

Variations in the number of active bacteria in the euphotic zone of a recently flooded reservoir

Jugnia¹,

Richardot²,

Debroas³

et al. 2000

Aquat. Microb. Ecol.

View full text Add to dashboard Cite

Using the CTC (5-cyano-2, 3-ditolyl tetrazolium chloride) method, we estimated the active fraction of bacterioplankton in the euphotic zone of the recently flooded Sep Reservoir, France, with the aim of evaluating its importance and dynamics in relation to temperature and some components of dissolved organic matter (DOM) (i.e. dissolved combined amino acids: DCAA; dissolved free monosaccharides: DFCHO; dissolved combined monosaccharides: DCCHO), and its metabolic significance in conjunction with a bacterial growth indicator:14 C-glucose uptake. From our results, it appears that only a small fraction (0.04 to 3.23%) of total bacterial count in this reservoir was metabolically active. Recorded correlations and multivariate regression analysis suggest that temporal variations in the number of active bacteria are mainly (80%) governed by temperature, together with the high concentrations of those components of DOM, which were present in this ecosystem but which apparently were not always available for bacterial metabolism. We also found a positive relation between the number of active bacteria and the potential heterotrophic activity, which suggest that CTC-positive bacteria are responsible for the bulk of bacterial community metabolism in this recently formed reservoir.KEY WORDS: Active bacteria · CTC · Dissolved organic matter · Temperature · Bacterial activity · New reservoir Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 22: [251][252][253][254][255][256][257][258][259] 2000 fraction of bacterioplankton (e.g. Thom et al. 1993), whereas others have suggested temperature (Tabor & Neihof 1984, Robarts & Sephton 1988, Sommaruga & Conde 1997, lysis (Proctor & Fuhrman 1990), grazing (Gasol et al. 1995) and resource availability (del Giorgio & Scarborough 1995, Choi et al. 1999. In terms of resource availability, a wide variety of sources including phytoplankton excretion and lysis (Cole et al. 1988, Maurin et al. 1997) and grazing and release by zooplankton (e.g. Hygum et al. 1997) have been identified as producing dissolved organic matter (DOM) that fuels bacterioplankton metabolism. Inputs of allochthonous DOM and physical disaggregation of detrital particles (Hadas et al. 1990) are also likely to be significant energy sources for bacterial activities. However, nearly all these studies have been conducted in natural or stabilized artificial lake ecosystems. To our knowledge, no information is available on fluctuations in the number of active bacteria in recently flooded reservoirs that are expected to contain large quantities of terrestrial detritus and organic matter, which is known to efficiently regulate bacterial activity (Paterson et al. 1997).The aim of this study was to determine the importance of the active fraction of bacterioplankton in a recently flooded reservoir, the main variables controlling their abundance (especially temperature and DOM) and their metabolic significance in conjunction with a growth indicator:14 C-glucose uptake. The numbe...

show abstract

“…The sum of both fractions (free-living bacteria and attached bacteria) is referred to total BPP. The amount of incorporated 14 C-Leu was converted into BPP by using an intracellular isotope dilution factor of 2 (Simon and Rosenstock, 1992). A conversion factor of 0.86 was used to convert the protein produced into carbon (Simon and Azam, 1989).…”

Section: Bacterial Production (Bpp)mentioning

confidence: 99%

Effects of CO<sub>2</sub> perturbation on phosphorus pool sizes and uptake in a mesocosm experiment during a low productive summer season in the northern Baltic Sea

et al. 2016

View full text Add to dashboard Cite

Abstract. Studies investigating the effect of increasing CO 2 levels on the phosphorus cycle in natural waters are lacking although phosphorus often controls phytoplankton development in many aquatic systems. The aim of our study was to analyse effects of elevated CO 2 levels on phosphorus pool sizes and uptake. The phosphorus dynamic was followed in a CO 2 -manipulation mesocosm experiment in the Storfjärden (western Gulf of Finland, Baltic Sea) in summer 2012 and was also studied in the surrounding fjord water. In all mesocosms as well as in surface waters of Storfjärden, dissolved organic phosphorus (DOP) concentrations of 0.26 ± 0.03 and 0.23 ± 0.04 µmol L −1 , respectively, formed the main fraction of the total P-pool (TP), whereas phosphate (PO 4 ) constituted the lowest fraction with mean concentration of 0.15 ± 0.02 in the mesocosms and 0.17 ± 0.07 µmol L −1 in the fjord. Transformation of PO 4 into DOP appeared to be the main pathway of PO 4 turnover. About 82 % of PO 4 was converted into DOP whereby only 18 % of PO 4 was transformed into particulate phosphorus (PP). PO 4 uptake rates measured in the mesocosms ranged between 0.6 and 3.9 nmol L −1 h −1 . About 86 % of them was realized by the size fraction < 3 µm. Adenosine triphosphate (ATP) uptake revealed that additional P was supplied from organic compounds accounting for 25-27 % of P provided by PO 4 only. CO 2 additions did not cause significant changes in phosphorus (P) pool sizes, DOP composition, and uptake of PO 4 and ATP when the whole study period was taken into account. However, significant short-term effects were observed for PO 4 and PP pool sizes in CO 2 treatments > 1000 µatm during periods when phytoplankton biomass increased. In addition, we found significant relationships (e.g., between PP and Chl a) in the untreated mesocosms which were not observed under high f CO 2 conditions. Consequently, it can be hypothesized that the relationship between PP formation and phytoplankton growth changed with CO 2 elevation. It can be deduced from the results, that visible effects of CO 2 on P pools are coupled to phytoplankton growth when the transformation of PO 4 into POP was stimulated. The transformation of PO 4 into DOP on the other hand does not seem to be affected. Additionally, there were some indications that cellular mechanisms of P regulation might be modified unPublished by Copernicus Publications on behalf of the European Geosciences Union. 3036M. Nausch et al.: Effects of CO 2 perturbation on phosphorus pool sizes der CO 2 elevation changing the relationship between cellular constituents.

show abstract

Carbon and nitrogen sources of planktonic bacteria in Lake Constance studied by the composition and isotope dilution of intracellular amino acids

Abstract: We investigated the physiological response of planktonic bacteria to varying C and N sources in mesotrophic Lake Constance (Bodensee) by studying the amino acid composition and the isotope dilution (ID) of [3H] amino acids in the intracellular pool.

Cited by 42 publications

References 29 publications

Links between bacterial production, amino-acid utilization and community composition in productive lakes

Links between bacterial production, amino-acid utilization and community composition in productive lakes

Variations in the number of active bacteria in the euphotic zone of a recently flooded reservoir

Effects of CO<sub>2</sub> perturbation on phosphorus pool sizes and uptake in a mesocosm experiment during a low productive summer season in the northern Baltic Sea

Contact Info

Product

Resources

About

Carbon and nitrogen sources of planktonic bacteria in Lake Constance studied by the composition and isotope dilution of intracellular amino acids

Abstract: We investigated the physiological response of planktonic bacteria to varying C and N sources in mesotrophic Lake Constance (Bodensee) by studying the amino acid composition and the isotope dilution (ID) of [3H] amino acids in the intracellular pool.

Cited by 42 publications

References 29 publications

Links between bacterial production, amino-acid utilization and community composition in productive lakes

Links between bacterial production, amino-acid utilization and community composition in productive lakes

Variations in the number of active bacteria in the euphotic zone of a recently flooded reservoir

Effects of CO&lt;sub&gt;2&lt;/sub&gt; perturbation on phosphorus pool sizes and uptake in a mesocosm experiment during a low productive summer season in the northern Baltic Sea

Contact Info

Product

Resources

About

Effects of CO<sub>2</sub> perturbation on phosphorus pool sizes and uptake in a mesocosm experiment during a low productive summer season in the northern Baltic Sea