Carbon-activated gas filtration during in vitro culture increased pregnancy rate following transfer of in vitro-produced bovine embryos

Merton, J.S.; Vermeulen, Z.L.; Otter, Tim; Mullaart, E.; Ruigh, L. de; Hasler, J.F.

doi:10.1016/j.theriogenology.2006.12.012

Cited by 57 publications

(33 citation statements)

References 14 publications

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“…For Czech Siemmental, Holstein Dairy and Beef Cattle cows, the mean proportion of transferable embryos derived from oocytes recovered in the growth phase of follicular development were 23.6%, 21.6% and 13.3%, respectively. These values are comparable with those reported by Galli et al (2003) who obtained embryo development rates of 21.8% and 15.6% in oocytes collected from culled genetically valuable cows and heifers of one herd, respectively, and by Merton et al (2007) who reached embryo development rates of 23.4 to 24.7% in oocytes collected by transvaginal aspiration from healthy pregnant heifers and first-parity Holstein Friesian cows.…”

Section: Discussionsupporting

confidence: 89%

“…Pregnancy rates of 28%, 30-40%, 32.8-39.2% and 35.6-40.8% were achieved by Dobrinsky (2002), Hernandez-Fonseca et al (2002), Hoshi (2003) and Merton et al (2007), respectively, after the transfer of frozen-thawed embryos produced in vitro from slaughterhouse-collected and transvaginally aspirated oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…It is generally known that the pregnancy rate after the transfer of cryopreserved embryos is lower than the rate after transfer of fresh embryos and that it is significantly lower after the transfer of frozen in vitro produced compared to the transfer of in vivo produced embryos (Hasler, 2001;Riha et al, 2002;Galli et al, 2003;Merton et al, 2007). Pregnancy rates of 28%, 30-40%, 32.8-39.2% and 35.6-40.8% were achieved by Dobrinsky (2002), Hernandez-Fonseca et al (2002), Hoshi (2003) and Merton et al (2007), respectively, after the transfer of frozen-thawed embryos produced in vitro from slaughterhouse-collected and transvaginally aspirated oocytes.…”

Section: Discussionmentioning

confidence: 99%

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In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Machatkova¹,

Hulinska²,

Rečková³

et al. 2008

Vet. Med. - Czech

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AbstrAct:In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and Beef Cattle breeds were used as oocyte donors. Oocytes were obtained either in the growth phase of the first follicular wave from cows with synchronized oestrus or in any other phases of follicular development from cows without oestrus synchronization. The embryos were prepared by a standard protocol described previously. The mean number of usable oocytes, transferable and freezable embryos per donor, and the mean percentage of usable, transferable and freezable embryos were assessed. The results were analyzed by Student's-t and Chi-squared tests. The embryos were frozen according to a slow freezing protocol. After thawing, they were transferred to recipients on Day 7 after oestrus. Irrespective of the breed, the mean numbers of usable oocytes and transferable and freezable embryos collected per donor were significantly higher (P < 0.01) for the synchronized than for the nonsynchronized donors (20.4 vs 11.7, 4.3 vs 1.0 and 3.2 vs 0.8, respectively). Similarly, the mean percentages of usable oocytes, transferable and freezable embryos were significantly higher (P < 0.01) for the synchronized than for the nonsynchronized donors (28.5% vs 20.5%, 20.9% vs 9.0% and 15.8% vs 6.5%, respectively). On comparison of the synchronized and nonsynchronized donors of each breed, the difference in the mean percentage of usable oocytes was significant (P < 0.01) in cows of all three breeds, the difference in the mean percentage of transferable embryos was significant in Czech Siemmental and Holstein Dairy cows (P < 0.01) and the difference in the mean percentage of freezable embryos was significant only in Holstein Dairy cows (P < 0.01). After the transfer of 41 frozen-thawed embryos and 43 fresh embryos, 20 heifers and 24 heifers became pregnant, respectively. In conclusion: (a) higher number of oocytes from infertile, genetically valuable cows was recovered in the growth phase compared with the other phases of follicular development; (b) greater development of these oocytes resulted in more embryos for transfer and cryopreservation; (c) the transfer of frozen-thawed and fresh embryos resulted in pregnancy rates of 48.8% and 55.8% , respectively.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Machatkova¹,

Hulinska²,

Rečková³

et al. 2008

Vet. Med. - Czech

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“…In addition, studies may fail to assess the relationship, or demonstrate correlation, between improvements in laboratory parameters and clinical outcome. One bovine breeding program reported a 5 % increase in pregnancy rate with use of an intra-incubator air filter system [8] but no changes in blastocyst formation rate or embryo quality.…”

Section: Introductionmentioning

confidence: 99%

Lack of carbon air filtration impacts early embryo development

Munch

Duran

Voorhis

2015

J Assist Reprod Genet

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Purpose To assess human fertilization and preimplantation embryo development in the presence and in the absence of carbon filtration Methods This is a retrospective cohort analysis of fresh, controlled ovarian hyperstimulation cycles as well as previously cryopreserved pronuclear stage embryo transfer cycles in a single IVF center. Embryo development and cycle-based outcomes were compared among three groups: 1) when carbon filtration was present, 2) when carbon filtration was absent, and 3) when carbon filtration had been restored. Results A total of 524 fresh cycles and 156 cryopreserved embryo cycles were analyzed. Fertilization, cleavage, and blastocyst conversion rates for fresh cycles all declined during the period of absent carbon filtration and recovered after the restoration of carbon filtration. Cryopreserved embryos that were thawed and cultured during the period of absent filtration did not have changes in cleavage or blastocyst conversion rates compared to periods where carbon filtration was present. Clinical pregnancy and live birth rates were unchanged among the three time periods.Conclusions The absence of carbon filtration in an IVF laboratory air handler is associated with poor fertilization and early embryo development for fresh cycles. Because development of previously frozen pronuclear stage embryos was unaffected, the lack of carbon filtration may preferentially affect embryos in the peri-fertilization period. Carbon filtration is an integral part to a successful human in-vitro fertilization laboratory.

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“…Levels of benzene, freons, aldehydes, toluene, acetone and isopropanol have been identified in compressed gas tanks [19]. Though charcoal activated filters in the incubator [22] or in the gas-line can remove some potential contaminants, impurities in atmosphere/gas composition directly from the tank or picked up during transit to the incubator through tubing, can still compromise embryo development. Chemically provided CO2 offers a potential solution to these impurities, as well as an alternate backup supply source of gas if needed due to inability to obtain/store cylinders of gas or equipment failure.…”

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confidence: 99%

A Self-Contained Culture Platform Using Carbon Dioxide Produced from a Chemical Reaction Supports Mouse Blastocyst Development In Vitro

Swain

2011

Journal of Reproduction and Development

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Abstract. Elevated CO2 is required for in vitro embryo culture to maintain proper media pH and to supply embryo metabolic pathways. As an alternative to current approaches using gas cylinders, we examined use of a chemical reaction to supply CO2. A closed culture system was constructed and chemicals added to generate CO2, which was then supplied to developing embryos. This system was shown to provide a stable pH (7.2-7.4) over 4 days of use. One-cell mouse embryos were cultured in the device and no difference in blastocyst formation or cell number was apparent between embryos grown in a closed system with CO2 supplied by a chemical reaction or positive controls grown in a an open system in a CO2 incubator. This approach provides a highly purified, inexpensive, and easily obtainable gas source and offers potential for development of new, self-contained culture platforms.

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Carbon-activated gas filtration during in vitro culture increased pregnancy rate following transfer of in vitro-produced bovine embryos

Cited by 57 publications

References 14 publications

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Lack of carbon air filtration impacts early embryo development

A Self-Contained Culture Platform Using Carbon Dioxide Produced from a Chemical Reaction Supports Mouse Blastocyst Development In Vitro

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