Carbohydrates present in the glycoprotein from conidia of the opportunistic pathogen Scedosporium prolificans

Gorin, Philip A.J.; Silva, M.I.D. da; Sassaki, Guilherme L.; Souza, Lauro Mera de; Wagner, Ricardo; Bittencourt, Vera Carolina B.; Simas, Fernanda Fogagnoli; Noseda, Miguel D.; Barreto-Bergter, E.

doi:10.1016/j.carbpol.2009.10.015

Cited by 9 publications

(11 citation statements)

References 11 publications

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“…The glycopeptide extracted from conidia of S. prolificans contained the same monosaccharide units as those of its mycelium, but with a trace of 2‐ O ‐methylrhamnose residues 10 …”

Section: Structural Aspects Of Peptidopolysaccharides and Polysaccharmentioning

confidence: 95%

“…1 shows the steps of purification routinely used in our laboratory for peptidopolysaccharide and polysaccharide extraction and purification. Hot aqueous extraction, followed by treatment with Cetavlon in the presence of sodium borate, provided a precipitate of peptidorhamnomannan (PRM), N ‐ and O ‐linked to peptide 8–11 . The carbohydrate moiety of P. boydii PRM was analysed using methylation‐GC‐MS analysis, partial acetolysis, 1 H and 13 C NMR spectroscopy and ESI‐MS.…”

Section: Structural Aspects Of Peptidopolysaccharides and Polysaccharmentioning

confidence: 99%

“…[3] that they are not respective teleomorph and anamorph of the same species. However, of the many different carbohydrate epitopes present in glycocomplexes of opportunistic, fungal pathogens P. boydii , 8 S. prolificans , 10 and now S. apiospermum , 12 an α‐Rha p ‐(1→3)‐α‐Man p ‐(1→2)‐α‐Man p ‐(1→ structural component is conserved.…”

Section: Structural Aspects Of Peptidopolysaccharides and Polysaccharmentioning

confidence: 99%

“…Hot aqueous extraction, followed by treatment with Cetavlon in the presence of sodium borate, provided a precipitate of peptidorhamnomannan (PRM), N-and O-linked to peptide. [8][9][10][11] The carbohydrate moiety of P. boydii PRM was analysed using methylation-GC-MS analysis, partial acetolysis, 1 H and 13 C NMR spectroscopy and ESI-MS. The PRM has a branched structure and contains a-Rhap-(1 fi 3)-a-Rhap-side-chain epitope linked (1 fi 3) to a (1 fi 6)-linked a-Manp core.…”

Section: Introductionmentioning

confidence: 99%

“…The 13 C NMR examination, as did methylation analysis, showed PRM-Sp to be different from PRM-Pb which indicated that PRM-Sp 11 The glycopeptide extracted from conidia of S. prolificans contained the same monosaccharide units as those of its mycelium, but with a trace of 2-O-methylrhamnose residues. 10 The O-linked oligosaccharides ( Fig. 2) were isolated from the PRMs of P. boydii, S. apiospermum and S. prolificans mycelium.…”

Section: Introductionmentioning

confidence: 99%

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Glycoconjugates and polysaccharides from the Scedosporium/Pseudallescheria boydii complex: structural characterisation, involvement in cell differentiation, cell recognition and virulence

Lopes

Silva

Bittencourt

et al. 2011

Mycoses

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SummaryPeptidorhamnomannans (PRMs), rhamnomannans and a-glucans are especially relevant for the architecture of the Scedosporium ⁄ Pseudallescheria boydii cell wall, but many of them are immunologically active, with great potential as regulators of pathogenesis and the immune response of the host. In addition, some of them can be specifically recognised by antibodies from the sera of patients, suggesting that they could also be useful in diagnosis of fungal infections. Their primary structures have been determined, based on a combination of techniques including gas chromatography, electrospray ionization -mass spectrometry (ESI-MS), 1 H-COSY and TOCSY, 13 C and 1 H ⁄ 13 C NMR spectroscopy. Using monoclonal antibodies to PRM, we showed that it is involved in germination and viability of P. boydii conidia, in the phagocytosis of P. boydii conidia by macrophages and non-phagocytic cells and in the survival of mice with P. boydii infection. Also, components of the fungal cell wall, such as a-glucans, are involved. Rhamnomannans are immunostimulatory and participate in the recognition and uptake of fungal cells by the immune system. These glycosylated polymers, being present in the fungal cell wall, are mostly absent from mammalian cells, and are excellent targets for the design of new agents capable of inhibiting fungal growth and differentiation of pathogens.

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“…The glycopeptide extracted from conidia of S. prolificans contained the same monosaccharide units as those of its mycelium, but with a trace of 2‐ O ‐methylrhamnose residues 10 …”

Section: Structural Aspects Of Peptidopolysaccharides and Polysaccharmentioning

confidence: 95%

Section: Structural Aspects Of Peptidopolysaccharides and Polysaccharmentioning

confidence: 99%

Section: Structural Aspects Of Peptidopolysaccharides and Polysaccharmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Glycoconjugates and polysaccharides from the Scedosporium/Pseudallescheria boydii complex: structural characterisation, involvement in cell differentiation, cell recognition and virulence

Lopes

Silva

Bittencourt

et al. 2011

Mycoses

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show abstract

Pathogenesis of Scedosporium

Lackner

Guarro

2013

Curr Fungal Infect Rep

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Toll-like receptors (TLR2 and TLR4) recognize polysaccharides of Pseudallescheria boydii cell wall

Figueiredo

Bittencourt

Lopes

et al. 2012

Carbohydrate Research

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Carbohydrates present in the glycoprotein from conidia of the opportunistic pathogen Scedosporium prolificans

Cited by 9 publications

References 11 publications

Glycoconjugates and polysaccharides from the Scedosporium/Pseudallescheria boydii complex: structural characterisation, involvement in cell differentiation, cell recognition and virulence

Glycoconjugates and polysaccharides from the Scedosporium/Pseudallescheria boydii complex: structural characterisation, involvement in cell differentiation, cell recognition and virulence

Pathogenesis of Scedosporium

Toll-like receptors (TLR2 and TLR4) recognize polysaccharides of Pseudallescheria boydii cell wall

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