Carbohydrates as a tool for oriented immobilization of antigens and antibodies

Functional Cellulose Beads: Preparation, Characterization, and Applications

“…Bioaffinity matrices for purification of either the antibody or its target have been prepared by coupling the corresponding counterpart to cellulose beads. 141,155,160,197,199,201 In general, immobilization of proteins proceeds by more or less random coupling of their functional amino acid residues with reactive groups on the cellulose beads. However, proteins are three-dimensional molecules with a very specific tertiary, even quaternary structure.…”

“…Reactive beads that can immobilize proteins and other amino group containing target molecules have been prepared by activation with cyanuric chloride ( 49 ). ,− Cellulose beads have also been converted with benzoquinones for the same purpose ( 41 ). , By reaction of beads with diisocyantes, isocyanate groups have been introduced ( 36 ). , These highly reactive moieties can either be hydrolyzed to amines or used to immobilize proteins via their amino groups. Amines could be coupled with cellulose beads containing carboxylic acid groups .…”

“…Also, other types of proteins have been immobilized on cellulose beads, such as antibodies and protein inhibitors, which possess well-defined binding sides for very specific proteins. Bioaffinity matrices for purification of either the antibody or its target have been prepared by coupling the corresponding counterpart to cellulose beads. ,,,,, …”

Prospects for the synthesis of hydrazine(hydrazine)-containing affinity type adsorbents (a review)

“…It was reported that AIBN can be used as an insert in ATA based on triazine-activated macroporous cellulose [92]. Upon activation of the carrier, AIBN was immobilized (0.05 M borate buffer, 4 h) with a capacity of about 12.7 mmole/(ml gel) [27].…”

“…Upon activation of the carrier, AIBN was immobilized (0.05 M borate buffer, 4 h) with a capacity of about 12.7 mmole/(ml gel) [27]. The resulting hydrazido-containing carrier was conjugated with oxidized ovalbumin [92]. Immobilization of estradiol through a triazine-activated hydrazido-containing carrier was described in [93].…”