Carbohydrates and Lipids in Trichinella spiralis Larvae and Their Utilization In vitro

Castro, Gilbert A.; Fairbairn, Donald

doi:10.2307/3277345

Cited by 120 publications

(41 citation statements)

References 16 publications

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“…The larvae were obtained from infected rodents 60 to 90 days postinfection (p.i. ), using a modification (50) of the technique described by Castro and Fairbairn (7). The mice were infected orally with 375 infective larvae and were allowed to recover until day 21 p.i.…”

Section: Methodsmentioning

confidence: 99%

Intestinal Nematode Infection Ameliorates Experimental Colitis in Mice

et al. 2002

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Epidemiological studies suggest that inflammatory bowel disease (IBD) is common in developed countriesIn the present study, we investigated the effect of polarizing the immune response toward the Th2 type, using intestinal nematode infection, on subsequent experimental colitis. Mice were infected with the intestinal nematode Trichinella spiralis and allowed to recover before colitis was induced with dinitrobenzene sulfonic acid. The mice were sacrificed postcolitis to assess colonic damage macroscopically, histologically, and by myeloperoxidase (MPO) activity and Th cytokines. Prior nematode infection reduced the severity of colitis both macroscopically and histologically together with a decreased mortality and was correlated with a downregulation of MPO activity, Th1-type cytokine expression in colonic tissue, and emergence of a Th2-type immune response. These results indicate a protective role of nematode infection in Th1 cell-driven inflammation and prompt consideration of a novel therapeutic strategy in IBD based on immunological distraction.

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Section: Methodsmentioning

confidence: 99%

Intestinal Nematode Infection Ameliorates Experimental Colitis in Mice

et al. 2002

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“…Rats were infected by administering 1.0 ml of 0.9% saline solution containing 7500 T. spiralis larvae by gavage. The larvae were obtained from female CD1 mice infected 30 to 90 days before as described previously (Castro and Fairbairn, 1969;Torrents and Vergara, 2000).…”

Section: Methodsmentioning

confidence: 99%

Mast Cell Stabilizer Ketotifen [4-(1-Methyl-4-piperidylidene)-4H-benzo[4,5]cyclohepta[1,2-b]thiophen-10(9H)-one Fumarate] Prevents Mucosal Mast Cell Hyperplasia and Intestinal Dysmotility in Experimental Trichinella spiralis Inflammation in the Rat

Serna

Porras

Vergara

2006

J Pharmacol Exp Ther

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Trichinella spiralis infection in rats induces hypermotility and an abnormal response to cholecystokinin (CCK) similar to motor disturbances observed in irritable bowel syndrome. Mast cell hyperplasia is also characteristic of this experimental model. The aim of our study was to correlate mast cell activity with the development of dysmotility and to demonstrate whether the mast cell stabilizer ketotifen [4-(1-methyl-4-piperidylidene)-4H-benzo [4,5]could prevent the development of intestine hypermotility. Sprague-Dawley rats were infected with T. spiralis and, 5 days after infection, treated with the mast-cell stabilizer ketotifen (10 mg/kg/day). Twelve days after infection, intestinal spontaneous motor activity and response to CCK were evaluated by means of strain-gauge transducers. Immunohistochemistry for rat mast cell protease II (RMCPII), cyclooxygenase (COX)-2, and inducible nitric-oxide synthase (iNOS) was performed in intestinal specimens. In addition, RMCPII and myeloperoxidase were determined in serum. Infected control rats showed hypermotility, mast cell hyperplasia, increased RMCPII levels, increased myeloperoxidase, and overexpression of COX-2 and iNOS. In contrast, ketotifen-treated rats showed spontaneous intestinal motility and CCK response similar to the noninfected control rats. Mast cell hyperplasia and RMCPII were reduced in ketotifen-treated rats. Inflammatory parameters were less modified by ketotifen, but those animals that received the longest ketotifen treatment showed a slight amelioration in these parameters. These results indicate that mast cells are implicated in the development of hypermotility. The treatment with ketotifen prevented hypermotility and mast cell hyperplasia and diminished mucosal mast cell activity.

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“…Rats were infected by administering 1.0 ml of 0.9% saline solution containing 7500 T. spiralis larvae by gavage. The larvae were obtained from female CD1 mice infected 30 to 90 days before by a modification of the method described by Castro and Fairbairn (1969).…”

Section: Methodsmentioning

confidence: 99%

Antinerve Growth Factor Treatment Prevents Intestinal Dysmotility inTrichinella spiralis-Infected Rats

Torrents

Torres²,

Mora³

et al. 2002

J Pharmacol Exp Ther

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Nerve growth factor (NGF) could be involved in the development of hyperalgesia as well as in nervous remodeling consequence of inflammation. Both dysmotility and increase of visceral sensitivity have been described in functional gastrointestinal disorders such as irritable bowel syndrome. Trichinella spiralis-infected rats show an exacerbated spontaneous motility and a significant increase of the excitatory response to cholecystokinin (CCK), both associated with a reversible inflammatory process and the hypertrophy of the muscle layers. In this study we determined the intestinal expression of NGF mRNA by polymerase chain reaction and NGF by enzyme-linked immunosorbent assay. We implanted serosal strain gauge transducers on duodenum, jejunum, and ileum of anesthetized Sprague-Dawley rats to record circular muscle contractions. The experimental protocol included the evaluation of intestinal spontaneous motor activity (SMA), the response to CCK-8, and the ascending contraction induced by electrical mucosal stimulation. This protocol was performed in healthy and infected nontreated rats, in healthy rats with an NGF antibody treatment (1.6 mg/rat i.p.), and in infected rats with the same treatment applied at 0 or 3 days postinfection. NGF and NGF mRNA levels in the bowel were increased during inflammation. Although anti-NGF treatments did not prevent or reverse inflammatory response, the treatment was effective in preventing the motor alterations induced by the T. spiralis infection, i.e., inhibited increased SMA, reversed altered response to CCK, and reversed in part exacerbated response to electrical stimulation.

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Carbohydrates and Lipids in Trichinella spiralis Larvae and Their Utilization In vitro

Cited by 120 publications

References 16 publications

Intestinal Nematode Infection Ameliorates Experimental Colitis in Mice

Intestinal Nematode Infection Ameliorates Experimental Colitis in Mice

Mast Cell Stabilizer Ketotifen [4-(1-Methyl-4-piperidylidene)-4H-benzo[4,5]cyclohepta[1,2-b]thiophen-10(9H)-one Fumarate] Prevents Mucosal Mast Cell Hyperplasia and Intestinal Dysmotility in Experimental Trichinella spiralis Inflammation in the Rat

Antinerve Growth Factor Treatment Prevents Intestinal Dysmotility inTrichinella spiralis-Infected Rats

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