Carbohydrate transport in <i>Clostridium pasteurianum</i>

Booth, Ian R.; Morris, Jonathan

doi:10.1007/bf01142198

Cited by 26 publications

(19 citation statements)

References 17 publications

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“…No change in the growth rate was observed when the second sugar was added, but rather there was an adjustment of the rate of utilization of the sugar initially present (data not shown). These results were consistent with constitutive phosphotransferase systems for glucose and fructose in C. pasteurianum (Booth & Morris, 1982), and indicated that, unlike other organisms (Kornberg, 1973;Amaral & Kornberg, 1975;Dills et al, 1980), C. pasteurianum has no sophisticated regulatory mechanism allowing differentiation between these two carbon sources.…”

Section: W J Mitchell and O T H E R Ssupporting

confidence: 77%

“…Induction of metabolism of the PTS substrate sorbitol was, however, prevented in the presence of glucose. The mechanism of regulation of sorbitol metabolism by glucose has been shown to involve inhibition of sorbitol accumulation by the cell (Booth & Morris, 1982;Mitchell et al, 1985), and a more extensive description of the mechanism of inhibition of sorbitol uptake by glucose will be presented elsewhere (M. S. Roohi & W. J. Mitchell, unpublished work).…”

Section: Resultsmentioning

confidence: 99%

“…The implication of these results is that, in uninduced cells, sorbitol is effectively excluded in the presence of glucose. Partial inhibition of sorbitol uptake by glucose and the analogue methyl aglucoside has in fact been demonstrated in fully induced cells (Booth & Morris, 1982;Mitchell et al, 1985). The extent of the inhibition is now known to be dependent on the ability of the sorbitol PTS to compete with the glucose PTS for a common energy pool (M. S. Roohi & W. J.…”

Section: W J Mitchell and O T H E R Smentioning

confidence: 99%

“…The sorbitol PTS has been reported to be constitutive in C. pasteurianum, but activity in glucose-grown cells was nonetheless extremely low (Booth & Morris, 1982). We therefore reexamined the activity of the sorbitol PTS in cultures growing exponentially on glucose, …”

Section: Utilization Of Glucose and Other Pts Sugarsmentioning

confidence: 99%

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Regulation of Carbohydrate Utilization in Clostridium pasteurianum

Mitchell¹,

Roohi²,

Mosely³

et al. 1987

Microbiology

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Clostridium pasteurianum is capable of fermentative growth on a number of carbohydrate compounds. Several, including glucose, fructose and sorbitol, are accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PTS), while the uptake of galactose and gluconate is protonmotive-force-dependent. We have examined the utilization of these substrates by cultures of C. pasteurianum growing on carbohydrate mixtures to determine whether the organism displays preferences for one carbon source over the others; such a preference may indicate the operation of specific mechanisms for regulation of carbohydrate metabolism. In most cases the carbohydrates were co-metabolized. Glucose was utilized together with fructose, gluconate and galactose, although galactose appeared to be favoured over glucose. This preference was not due to repression of synthesis of the glucose PTS. On the other hand, glucose prevented induction of the sorbitol PTS, and led to strong inhibition of sorbitol utilization in uninduced cells. Pre-adaptation of cells to growth on sorbitol counteracted the inhibition by glucose resulting in utilization of glucose and sorbitol at approximately equal rates.

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Section: W J Mitchell and O T H E R Ssupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: W J Mitchell and O T H E R Smentioning

confidence: 99%

Section: Utilization Of Glucose and Other Pts Sugarsmentioning

confidence: 99%

See 2 more Smart Citations

Regulation of Carbohydrate Utilization in Clostridium pasteurianum

Mitchell¹,

Roohi²,

Mosely³

et al. 1987

Microbiology

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“…Most sugars appear to be translocated in Clostridium spp. by phosphoenolpyruvate-dependent phosphotransferase systems (3,4).…”

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confidence: 99%

Amino acid transport by membrane vesicles of an obligate anaerobic bacterium, Clostridium acetobutylicum

1988

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Membrane vesicles were isolated from the obligate anaerobic bacterium Clostridium acetobutylicum. Beef heart mitochondrial cytochrome c oxidase was inserted in these membrane vesicles by membrane fusion by using the freeze-thaw sonication technique (A. J. M. Driessen, W. de Vrij, and W. N. Konings, Proc. Natl. Acad. Sci. USA 82:7555-7559, 1985) to accommodate them with a functional proton motive force-generating system. With ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine-cytochrome c as the electron donor, a proton motive force (delta p) of -80 to -120 mV was generated in these fused membranes. This delta p drove the accumulation of leucine and lysine up to 40- and 100-fold, respectively. High transport activities were observed in fused membranes containing Escherichia coli lipids, whereas the transport activities in fused membranes containing mainly soybean lipids or phosphatidylcholine were low. It is suggested that branched-chain amino acids and lysine were taken up by separate systems. The effects of the ionophores nigericin and valinomycin indicated that lysine and leucine were translocated in symport with a proton.

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Anaerobes, Industrial Uses

Jain¹,

Zeikus²

1999

Encyclopedia of Bioprocess Technology

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Carbohydrate transport in Clostridium pasteurianum

Cited by 26 publications

References 17 publications

Regulation of Carbohydrate Utilization in Clostridium pasteurianum

Regulation of Carbohydrate Utilization in Clostridium pasteurianum

Amino acid transport by membrane vesicles of an obligate anaerobic bacterium, Clostridium acetobutylicum

Anaerobes, Industrial Uses

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