Carbohydrate transport in bacteria.

Dills, S S; Apperson, A; Schmidt, Mary R.; Saier, Milton H.

doi:10.1128/mmbr.44.3.385-418.1980

Cited by 217 publications

(122 citation statements)

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“…Such a mechanism which couples transport of a substrate with its chemical modification is termed group translocation. P-enolpyruvate dependent sugar transport is found in most faculta- tive and obligatory anaerobic bacteria [3,4] but not in eucaryotes including yeast [5]. It is used by E. coli and Salmo~ella typhimurium for the transport of glucose, of other hexoses (e.g.…”

Section: Jj Different Mechanisms Of Glucose Transport and Their Dismentioning

confidence: 99%

Glucose transport in Escherichia coli

Erni

1989

FEMS Microbiology Reviews

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Section: Jj Different Mechanisms Of Glucose Transport and Their Dismentioning

confidence: 99%

Glucose transport in Escherichia coli

Erni

1989

FEMS Microbiology Reviews

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“…Glucose is transported in S. mutans by 2 known mechanisms, the high affinity P-enolpyruvate (PEP) phosphotransferase system (PTS) (10,29) and a lower affinity systetn (16). Earlier evidence (14,16) indicated that this latter system was coupled to proton motive force, but recent data suggest that glucose is taken up by simple diffusion (8).…”

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confidence: 99%

Sorbitol inhibition of glucose metabolism by Streptococcus sanguis 160

Hamilton

Svensäter

1991

Oral Microbiology and Immunology

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Clinical studies in Sweden have shown that the proportion of sorbitol-utilizing strains of Streptococcus sanguis increases in dental plaque from individuals using sorbitol-containing products for prolonged periods. We have undertaken to study the metabolism of glucose and sorbitol by S. sanguis 160, isolated from a subject consuming sorbitol-containing chewing-gum 4 times a day for 4 years. Growth on glucose was inhibited by the presence of sorbitol in the growth medium and sorbitol was utilized in the presence of glucose, albeit, at a slower rate than glucose. In addition, pulses of glucose added to cultures growing on sorbitol resulted in the expulsion of sorbitol from the cell. In order to examine further the relationship of sorbitol and glucose, uptake assays were carried out with S. sanguis 160 grown in continuous culture (pH 7.0, dilution rate = 0.1 h-1) with glucose, sorbitol or nitrogen (sorbitol excess) limitations. The uptake of [14C]-glucose by sorbitol-limited cells, but not by glucose-limited cells, was inhibited by sorbitol, as was glycolysis. Kinetic experiments with glucose-limited cells showed 2 transport systems for glucose with Ks values of 5.2 and 40 microM, and glucose phosphorylation activity by decryptified cells indicated transport by the P-enolpyruvate (PEP) phosphotransferase system (PTS) with lesser activity for an ATP-dependent transport process. Transition from glucose-limited growth to sorbitol-limited growth revealed repression of total [14C]-glucose uptake by intact cells and activity for Enzyme II for glucose (Ellglc) of the PTS measured in membrane preparations in the presence of an excess of the soluble PTS proteins in crude cell-free supernatant fractions.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…TMG has a structure similar to that of IPTG, and like IPTG, TMG is a gratuitous inducer of the lac operon (Miller, 1978). TMG has been used extensively in the characterization of lac permease (Dills et al, 1980). Three mutant RB79l(pKN) cell colonies and two original RB79l(pKN) cell colonies were each cured of their plasmid.…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli Host Cell Modifications in Continuous Culture Affecting Heterologous Protein Overproduction: A Population Dynamics Study

Togna

Shuler

et al. 1992

Biotechnology Progress

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There are many published studies of plasmid segregational instability in Escherichia coli in the literature. However, the formation of plasmid-free segregants can be controlled by the addition of selective chemical agents like antibiotics. This solution has become commonplace in both the laboratory and industry. On the other hand, host cell modifications, which result in low production of plasmid-encoded protein and lead to loss of culture productivity, have not been adequately addressed. Continuous culture of an inducible (ptac) Escherichia coli vector containing strain, RB791(pKN), was characterized by strong dynamic changes in the cell population and product (beta-lactamase) expression. Long-term cultivation resulted in the loss of high-level production of beta-lactamase. Loss of productivity was not due to the formation of plasmid-free cells or structural modifications to the plasmid; instead, continuous operation resulted in a culture dominated by irreversibly altered, low-producing cells. Two distinct classes of lac- mutants which inhibited induction were identified (Y- and I(s)).

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Carbohydrate transport in bacteria.

Cited by 217 publications

References 110 publications

Glucose transport in Escherichia coli

Glucose transport in Escherichia coli

Sorbitol inhibition of glucose metabolism by Streptococcus sanguis 160

Escherichia coli Host Cell Modifications in Continuous Culture Affecting Heterologous Protein Overproduction: A Population Dynamics Study

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