The stable C isotope composition (δC) of leaf and wood tissue has been used as an index of water availability at both the species and landscape level. However, the generality of this relationship across species has received little attention. We compiled literature data for a range of conifers and examined relationships among landscape and environmental variables (altitude, precipitation, evaporation) and δC. A significant component of the variation in δC was related to altitude (discrimination decreased with altitude in stemwood, 2.53‰ km altitude, r =0.49, and in foliage, 1.91‰ km, r =0.42), as has been noted previously. The decrease in discrimination with altitude was such that the gradient in CO partial pressure into the leaf (P -P) and altitude were generally unrelated. The ratio of precipitation to evaporation (P/E) explained significant variation in P -P of stemwood (r =0.45) and foliage (r=0.27), but only at low (<0.8) P/E. At greater P/E there was little or no relationship, and other influences on δC probably dominated the effect of water availability. We also examined the relationship between plant drought stress (Ψ) and δC within annual rings of stemwood from Pinus radiata and Pinus pinaster in south-western Australia. Differential thinning and fertiliser application produced large differences in the availability of water, nutrients and light to individual trees. At a density of 750 stems ha, Ψ and δC were less (more negative) than at 250 stems ha indicating greater drought stress and less efficient water use, contrary to what was expected in light of the general relationship between discrimination and P/E. The greater δC of trees from heavily thinned plots may well be related to an increased interception of radiation by individual trees and greater concentrations of nutrients in foliage - attributes that increase rates of photosynthesis, reduce P and increase δC. δC was thus modified to a greater extent by interception of radiation and by nutrient concentrations than by water availability and the δC-Ψ relationship varied between thinning treatments. Within treatments, the relationship between δC and Ψ was strong (0.38