Carbohydrate Metabolism during Sporulation in Spheroplasts of Yeast,Saccharomyces cerevisiae

Ca(2+)-induced sporulation in the fungus Achlya : the biological roles of [Beta]-(1->3)-glucans and key enzymes /

“…Therefore, sporulation may provide a convenient system for the investigation of carbohydrate metabolism, the roles of the reserve materials and the associated hydrolytic enzymes. In yeast the extraspore reserve carbohydrates synthesized early in development are completely degraded prior to spore formation, suggesting they have a role as a source of energy and carbon for spore formation (Rothman and Cabib, 1969;Becker et al, 1982;Katohda et al, 1988;Rua et al, 1993 consisting of a neutral form (20%) and a phosphorylated form (80%) (Lee et al, 1996). Under conditions of starvation ( no glucose in the culture medium ) or Ca -induced sporulation, the myceUal glucans decrease dramatically, and their total phosphate content decreases 49% (Lee and Mullins, 1994).…”

“…Sporulation provides a convenient system for the investigation of carbohydrate metabolism and the utilization of reserve carbohydrates. In yeast reserve carbohydrates synthesized early in development were completely degraded prior to spore formation, suggesting them as a source of energy and carbon to support sporulation (Rothman and Cabib, 1969;Becker et al, 1988;Katohda et al, 1988;Rua et al, 1993). In Achlva.…”

Direct Visualization of Structurally Similar Polysaccharides in Single Yeast Cells in Vivo by Multivariate Analysis Assisted Raman Microspectroscopy

“…Glucan accumulation refers to the buildup of these polysaccharides inside cells. This can occur due to a variety of factors, including high levels of glucose in the growth medium, mutations in genes involved in glucan metabolism, or the presence of certain types of enzymes . Sometimes, when glucan accumulates in yeast cells, it can lead to several problems.…”

“…This can occur due to a variety of factors, including high levels of glucose in the growth medium, mutations in genes involved in glucan metabolism, or the presence of certain types of enzymes. 26 Sometimes, when glucan accumulates in yeast cells, it can lead to several problems. One of the most significant is that it can make the cells more resistant to certain types of stress, such as high temperature or exposure to ethanol.…”