Carbohydrate-Mediated Biomolecular Recognition and Gating of Synthetic Ion Channels

Ali, Mubarak; Nasir, Saima; Ramı́rez, Patricio; Cervera, Javier; Mafé, Salvador; Ensinger, Wolfgang

doi:10.1021/jp4054555

Cited by 65 publications

(66 citation statements)

References 63 publications

(97 reference statements)

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“…This fact clearly suggests that soft matter nanostructures operating in an aqueous ionic solution can effectively be coupled to conventional electronic elements like commercial capacitors. Because of the biomimetic nature of the pore, the results have clear implications for sensing and information processing using bioelectronic interfaces and nanopore-based biosensors [4][5][6][7][8][9][10][11]. We note in this context that the artificial nanopore used here shows electrical rectification and ionic selectivity characteristics similar to those observed in wide ion channels (e.g., the bacterial porin OmpF of Escherichia coli, a protein pore reconstituted on a planar lipid bilayer [12,13]).…”

Section: Introductionmentioning

confidence: 70%

Energy conversion from external fluctuating signals based on asymmetric nanopores

et al. 2015

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ElsevierRamirez Hoyos, P.; Gómez Lozano, V.; Cervera, J.; Nasir, S.; Ali, M.; Ensinger, W.; Mafe, S. (2015). Energy conversion from external fluctuating signals based on asymmetric nanopores. Nano Energy. 16:375-382. doi:10.1016Energy. 16:375-382. doi:10. /j.nanoen.2015 AbstractElectrical transduction from fluctuating external signals is central to energy conversion based on nanoscale electrochemical devices and bioelectronics interfaces. We demonstrate theoretically and experimentally a significant energy transduction from white noise signals using the electrical rectification of asymmetric nanopores in polymeric membranes immersed in aqueous electrolyte solutions. Load capacitor voltages of the order of 1 V are obtained within times of the order of 1 min by means of nanofluidic diodes which convert zero time-average potentials of amplitudes of the order of 1 V into average net currents. We consider singlenanopore and multipore membranes to show that the conversion processes can be significantly increased by scaling. The results concern a wide range of operating electrolyte concentrations.Because these concentrations dictate the pore resistance, the tuning of the load capacitance to optimize the system response at each concentration is also addressed. Finally, the experimental results are described theoretically by using simple equivalent circuits with a voltage-dependent resistance in series with a load capacitance.

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Section: Introductionmentioning

confidence: 70%

Energy conversion from external fluctuating signals based on asymmetric nanopores

et al. 2015

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“…Those biomolecular conjugations can be achieved by the immobilization, on the inner wall of the nanopore, of recognition sites for biomolecules such as, aptamer, [23] biotin [24][25][26], Nitrilotriacetic acid (NTA) [27] or antibodies [28]. Multistep functionalization using proteins have been performed with multipore membranes in order to detect, T4 Polynucleotide Kinase [29] or glucose [30] as well as to evaluate enzymatic reaction under confinement [31].…”

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confidence: 99%

Non-Fluorescence label protein sensing with track-etched nanopore decorated by avidin/biotin system

Lepoitevin

Bechelany

Balanzat

et al. 2016

Electrochimica Acta

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“…Note that the net average currents hIi are of similar order of magnitude as the currents I. These results could be enhanced in practical applications by using multi-pore membranes, 33 (Fig. 3(b)).…”

Section: Fig 1 Schemes Of the Charged Nanoparticle And The Cylindrimentioning

confidence: 69%

Nanoparticle-induced rectification in a single cylindrical nanopore: Net currents from zero time-average potentials

Ali

Ramı́rez

Nasir

et al. 2014

Applied Physics Letters

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Rectification in nanopores is usually achieved by a fixed asymmetry in the pore geometry and charge distribution. We show here that nanoparticle blocking of a cylindrical pore induces rectifying properties that can support significant net currents with zero time-average potentials. To describe experimentally this effect, the steady-state current-voltage curves of a single nanopore are obtained for different charge states and relative sizes of the pore and the charged nanoparticles, which are present only on one side. The rectification phenomena observed can find applications in the area of nanofluidics and involves physical concepts that are also characteristic of the blocking of protein ion channels by ionic drugs. Net currents can be obtained with zero time-average forces in spatially asymmetric systems, 1-3 e.g., the ion channels inserted in biological membranes and nanofluidic diodes. [4][5][6][7] We have recently shown this effect in the pumping of potassium ions against an external concentration gradient imposed on the bacterial porin OmpF (outer membrane protein F) channel of Escherichia coli 5 as well as in a single asymmetric nanopore functionalized with amino acid groups. 4 We demonstrate in this Letter nanoparticle-induced rectification in a cylindrical pore using fundamental concepts of nanofluidics.Nanopores track-etched in polymer films are more robust than ion channels and can be functionalized with electrically active moieties whose charge can be modulated externally through electrical, thermal, chemical, and optical signals, resulting in liquid-state transistors useful for separation, energy conversion, and chemical information processing. [8][9][10][11][12] We are concerned with a nanoparticle-induced gating that supports rectification in a cylindrical nanopore (Fig. 1), as opposed to the usual case of an asymmetric pore geometry and charge distribution. 4,7,[11][12][13][14] The nanopore is symmetric but the system as a whole is not completely symmetric because the charged nanoparticles are present only on one side of the pore.Nanoparticle blocking and release has previously been used for stochastic electrophoretic capture at the tip of conical pores using a scanning electrochemical microscope. 14,15 However, our aim here is not to obtain the particle characteristics from the individual blocking and release events recorded in the current traces of an asymmetric membrane.14,15 Rather, we will show that net currents can result from zero time-average forces in a single cylindrical nanopore because of partial blocking of the pore. We demonstrate this effect for different charges and relative sizes of the nanostructures (see Fig. 1) allowing significant blocking for small pores (r > R) and particle translocation for large pores (r < R). The obvious case r ) R was not considered because we wanted to show that it is possible to tune the pore diameters in the experimental range R min < r < R max and obtain significant blocking and rectification effects.The results are of interest not only to nanofluidi...

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Carbohydrate-Mediated Biomolecular Recognition and Gating of Synthetic Ion Channels

Cited by 65 publications

References 63 publications

Energy conversion from external fluctuating signals based on asymmetric nanopores

Energy conversion from external fluctuating signals based on asymmetric nanopores

Non-Fluorescence label protein sensing with track-etched nanopore decorated by avidin/biotin system

Nanoparticle-induced rectification in a single cylindrical nanopore: Net currents from zero time-average potentials

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