Carbohydrate interference in assays based on the periodate-coupled thiobarbituric acid reagent

Kuwahara, Steven S.

doi:10.1016/0003-2697(80)90039-1

Cited by 21 publications

(5 citation statements)

References 15 publications

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“…One major drawback of this method is that the acid treatment of intact cells also releases other cellular components such as 2-deoxyribose (Warren, 1959), glycosides and fatty acids (Kuwahara, 1980) that can interfere with the subsequent thiobarbiturate reaction. To override the interference, a correction control is needed (Rajalakshmi & Prasad, 1968).…”

Section: Introductionmentioning

confidence: 99%

Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis

Toowicharanont¹,

Chulavatnatol²

1983

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Summary. Bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mMNaIO4 at 0\ s=deg\ C, liberating C-9 as formaldehyde which was further quantitated using 3 \ x = r e q -\ methyl-2-benzothiazolinone. The mean \ m=+-\ s.d. (n = 20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50\m=.\9 \m=+-\ 8\m=.\0and 25\m=.\2 \m=+-\ 3\ m=. \ 8 nmol/108 spermatozoa respectively. About 85% of the former and 75% of the latter could be extracted by 1% Triton X-100 and 2 mM-dithiothreitol. About 70% of the former and 20% of the latter were released by neuraminidase from Vibrio cholerae. About 40% of the former and 30% of the latter were sensitive to trypsin. During sperm maturation, the decrease in the total bound sialic acids was due to the decrease in the neuraminidase-sensitive but not the neuraminidase-resistant sialic acids.

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Section: Introductionmentioning

confidence: 99%

Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis

Toowicharanont¹,

Chulavatnatol²

1983

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“…The thiobarbituric method of Aminoff (1961) is prone to interferences from proteins and from other substances which generate chromophores with thiobarbituric acid (Sailer and Gerstenfeld, 1962;Kuwahara, 1980;Cardo et al, 1985) when assaying biological samples such as cell extracts or urine. Attempts have been made to remove the proteins, but these tend to involve several processing steps such as in Saifer and Gerstenfeld (1962) where trichloroacetic acid precipitation was used.…”

Section: Quantitation Of Sialic Acidmentioning

confidence: 99%

“…In order to reduce the level of interference by other substances which the polyethylene glycol-Dowex 1 treatment does not remove, and which absorb at 530nm, and to avoid the need for tedious extraction procedures (Saifer and Gerstenfeld, 1962;Hammond and Papermaster, 1976;Kuwahara, 1980;Roboz et al, 1981;Cardo et al, 1985), the second derivative spectrum (D2) was taken over the wavelength range 510-590nm. The second derivative method allows absorbances at specific wavelengths to be discerned amongst other neighbouring absorbances and useful spectra can even be obtained from samples containing particulate matter which scatters light (Perkin Elmer, Lamda 5 manual).…”

Section: Quantitation Of Sialic Acidmentioning

confidence: 99%

Biochemical characterization of patients and prenatal diagnosis of sialic acid storage disease for three families

Clements

Taylor

Hopwood

1987

J of Inher Metab Disea

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Modifications of the assay method of Aminoff (1961) for the determination of sialic acid levels in urine, amniotic fluid, cultured cell homogenates and tissue homogenates, which reduce the interference from proteins by precipitation and from interfering chromogens by second derivative spectroscopy are described. Biochemical profiles of patients from three families with different clinical forms of sialic acid storage disease were made using data obtained with the new method. A family with two patients with the clinically severe, early-onset form is described, in which a 9-fold elevation of sialic acid can be detected in the skin fibroblasts and a 12-fold elevation in the urine. A patient from the second family is described with very severe clinical features and with 160-fold and 16-fold elevations of sialic acid in the urine and skin fibroblasts respectively. A patient from a third family is described with mild clinical features but with a 160-fold and 6-fold elevation of sialic acid in urine and skin fibroblasts respectively. The data obtained in this study are compared with data obtained in other laboratories on other patients. The method was used to assess the levels of sialic acid present in amniotic cells and chorionic villus cells obtained prenatally from pregnancies in each of the three families. In one case, in which amniotic cells were elevated in sialic acid and were vacuolated, the pregnancy was terminated. Follow-up studies confirmed the diagnosis. Pregnancies from the other two families were assessed to be not affected.

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“…Although there have been several reports in the literature concerning interference of 2-keto-3-deoxyoctonate (KDO) with assays developed for the determination of sialic acid (Kuwahara and Snetting, 1979;Kuwahara, 1980), KOO did not interfere with the determination of NANA by the HCl-resorcinol method {see appendix, Figure 29). A comparative analysis of the synthesis and release kinetics of sialic acid during growth revealed that sialic acid was.…”

mentioning

confidence: 99%

“…strains M986 and PRM102 in batch cultures of NDM-C medium. It is important to note that although there have been several reports in the literature concerning interference of 2-keto-3-deoxyoctonate (KDO) with assays developed for the determination of sialic acid(Kuwahara and Snetting, 1979;Kuwahara, 1980), KDO did not interfere with the determination of NANA by the HCl-resorcinol method (see appendix,Figure 29). A comparative analysis of the synthesis and release kinetics of sialic acid during growth revealed that sialic acid was produced during the exponential phase of growth of strain M986 and significant release of the polysaccharide to the surrounding medium occurred only when the culture has entered the stationary phase of growth (Figure 2).…”

mentioning

confidence: 99%

Physiology of Sialic Acid Capsular Polysaccharide Synthesis in Serogroup B Neisseria meningitidis

1983

J Bacteriol

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Carbohydrate interference in assays based on the periodate-coupled thiobarbituric acid reagent

Cited by 21 publications

References 15 publications

Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis

Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis

Biochemical characterization of patients and prenatal diagnosis of sialic acid storage disease for three families

Physiology of Sialic Acid Capsular Polysaccharide Synthesis in Serogroup B Neisseria meningitidis

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