Carbohydrate Fermentation Test of Lactic Acid Starter Cultures

Abstract

The sugar fermentation activity of lactic acid bacteria plays a significant part in the production of plant-based fermented foods. Lactic acid bacteria are demanding on nutrients in the medium. They generate energy only during the breakdown of carbohydrates. Lactic acid bacteria do not ferment polysaccharides like starch ordextrinsbecause they lack the necessary hydrolytic enzymes. Lactose is absent in plants, but this sugar is important for the growth of lactic acid bacteria. Due to the increased demand for the alternative fermented dairy products, it is necessary to know the ability of starter culturesto ferment various carbohydrates. The results of a study of the sugar fermenting activity of bacterial cultures, including the species S.thermophilus, L. delbruckii ssp. bulgaricus, L. lactis ssp. lactis, L. lactis ssp. cremoris, L. lactis ssp. lactis biovar diacetylactis, Leu. mesenteroides ssp. cremoris, and Leu. mesenteroides ssp. mesenteroidesare presented.Cultures with S.thermophilusand L. delbruckii ssp. bulgaricus show the lowest growth rates on maltose medium. Cultures with Leu. Mesenteroides rapidly ferment sucrose rapidly. Cultures withL. lactis ssp. lactis, L. lactis ssp. cremoris, and L. lactis ssp. lactis biovar diacetylactis rapidly metabolize maltose, sucrose, and lactose. All lactic acid bacteria rapidly utilize glucose.

Ligilactobacillus murinus Strains Isolated from Mice Intestinal Tract: Molecular Characterization and Antagonistic Activity against Food-Borne Pathogens

“…Based on the fermentation type, lactobacilli are classified into three groups: obligately homofermentative, fermenting via the glycolytic pathway; facultative heterofermentative, fermenting via the glycolytic/pentose phosphoketolase pathway and obligately heterofermentative, fermenting via the pentose phosphoketolase pathway. Therefore, obligately homofermentative lactobacilli cannot assimilate pentoses [37]. However, there are exceptions for some strains, such as the homolactic fermentation of a pentose, for instance, phosphoketolase is induced in the presence of xylose, and the metabolism may shift between these two pathways according to the xylose concentration in the medium [38].…”

Proceedings of the 9th International Conference on Advanced Materials and Systems

“…The ability of bacterial isolates to utilize different carbohydrates was tested with long "Hiss rows" with the use of 9 carbohydrate substrates: glucose, fructose, mannose, galactose, arabinose, xylose, ribose, lactose, sucrose (Gunkova et al, 2021).…”

Renewable Carbohydrates: Advancements in Sustainable Glucose Production and Optimization

“…They possess the ability to metabolize glucose through the glycolysis pathway, producing ethanol as the primary end product. Other microorganisms, such as lactic acid bacteria or certain species of Escherichia coli, can ferment sugars to produce organic acids like lactic acid or acetic acid (Gunkova et al, 2021). The selection of microorganisms depends on the desired end product and the specific requirements of the fermentation process (Behera et al, 2019).…”