Carbohydrate composition and identification of blood group A, B, and H oligosaccharide structures on human Factor VIII/von Willebrand factor.

Sodetz, James M.; Paulson, James C.; McKee, Patrick A.

doi:10.1016/s0021-9258(19)86586-9

Cited by 103 publications

(9 citation statements)

References 29 publications

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“…Exact protein concentrations were determined by quantitative amino acid analysis. Amino sugars were measured after hydrolysis in 2 N HC1 at 100 °C, deproteinization on Dowex 1-X2 in 70% methanol, and amino acid analysis using a modified buffer system (Sodetz et al, 1979). A trace amount of [U-14C]glucosamine was included as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

Complementary DNA and derived amino acid sequence of the .alpha. subunit of human complement protein C8: evidence for the existence of a separate .alpha. subunit messenger RNA

Rao

Howard²,

Ng³

et al. 1987

Biochemistry

110

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The entire amino acid sequence of the alpha subunit (Mr 64,000) of the eighth component of complement (C8) was determined by characterizing cDNA clones isolated from a human liver cDNA library. Two clones with overlapping inserts of net length 2.44 kilobases (kb) were isolated and found to contain the entire alpha coding region [1659 base pairs (bp)]. The 5' end consists of an untranslated region and a leader sequence of 30 amino acids. This sequence contains an apparent initiation Met, signal peptide, and propeptide which ends with an arginine-rich sequence that is characteristic of proteolytic processing sites found in the pro form of protein precursors. The 3' untranslated region contains two polyadenylation signals and a poly(A) sequence. RNA blot analysis of total cellular RNA from the human hepatoma cell line HepG2 revealed a message size of approximately 2.5 kb. Features of the 5' and 3' sequences and the message size suggest that a separate mRNA codes for alpha and argues against the occurrence of a single-chain precursor form of the disulfide-linked alpha-gamma subunit found in mature C8. Analysis of the derived amino acid sequence revealed several membrane surface seeking domains and a possible transmembrane domain. These occur in a cysteine-free region of the subunit and may constitute the structural basis for alpha interaction with target membranes. Analysis of the carbohydrate composition indicates 1 or 2 asparagine-linked but no O-linked oligosaccharide chains, a result consistent with predictions from the amino acid sequence.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Methodsmentioning

confidence: 99%

Complementary DNA and derived amino acid sequence of the .alpha. subunit of human complement protein C8: evidence for the existence of a separate .alpha. subunit messenger RNA

Rao

Howard²,

Ng³

et al. 1987

Biochemistry

110

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“…Dialysis was performed using cellulose membranes with a molecular weight cutoff of 3 kDa with water as solvent. 1 H NMR spectra were recorded at room temperature with a Varian INOVA 300 MHz spectrometer. In all cases, the sample concentration was 10 mg mL -1 , and the appropriate deuterated solvent was used as an internal standard.…”

Section: Experimental Materials and Methodsmentioning

confidence: 99%

“…3A), comparison of the integrated signal from the phenyl protons (7.04 ppm, H 2¢,6¢ and 7.22 ppm, H 3¢,5¢ ) with that of the anomeric protons of lactose (4.36 ppm, H 1¢-Lact and 4.43 ppm, H 1-Lact ), as well as that of the polymer backbone methine (2.10 ppm, CH) and methylene (1.55 ppm, CH 2 ), indicated an average glycopolymer composition of 10 lactose and 70 acrylamide units. The molecular weight (M n ) was about 7,000 as determined by 1 H NMR. Furthermore, the grafted carbohydrate on glycopolymer 4 was quantified via the phenol-sulfuric acid method.…”

Section: One-pot Synthesis and Characterization Of The O-cyanate Chai...mentioning

confidence: 99%

Orientated glyco-macroligand formation based on site-specific immobilization of O-cyanate chain-end functionalized glycopolymer

Narla

Sun

2011

Org. Biomol. Chem.

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We report an oriented glyco-macroligand formation and its glyco-affinity capturing and glycoarray application by combining an O-cyanate chain-end functionalized glycopolymer with commercially available amine-functionalized silica gel and glass slides via isourea bond formation. The O-cyanate chain-end functionalized glycopolymer was synthesized via cyanoxyl-mediated free-radical polymerization (CMFRP) in a one-pot fashion. The oriented glyco-macroligand formation was conducted under mild aqueous conditions at room temperature and was confirmed with fluorescence imaging after specific lectin binding, and will find important biomedical applications such as in biosensors and glycoarrays. In addition, the O-cyanate chain-end functionalized glycopolymer can also be used for site-specific polymer-protein conjugation.

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“…Carbohydrates are one of the most diverse and important classes of natural products. Carbohydrate–protein interactions are involved in numerous physiological and pathological processes such as cell–cell signaling and blood-type recognition and also play key roles in oncogenesis, bacterial/viral infection, fertilization, and many other health and disease processes . A major obstacle to the study of the biological roles of carbohydrates is accessing sufficient amounts of pure and structurally defined oligosaccharides.…”

mentioning

confidence: 99%

Conformationally Constrained Glycosyl Donors as Tools to Control Glycosylation Outcomes

2020

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Carbohydrate composition and identification of blood group A, B, and H oligosaccharide structures on human Factor VIII/von Willebrand factor.

Cited by 103 publications

References 29 publications

Complementary DNA and derived amino acid sequence of the .alpha. subunit of human complement protein C8: evidence for the existence of a separate .alpha. subunit messenger RNA

Complementary DNA and derived amino acid sequence of the .alpha. subunit of human complement protein C8: evidence for the existence of a separate .alpha. subunit messenger RNA

Orientated glyco-macroligand formation based on site-specific immobilization of O-cyanate chain-end functionalized glycopolymer

Conformationally Constrained Glycosyl Donors as Tools to Control Glycosylation Outcomes

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