Carbohydrate Catabolism in Pseudomonas aeruginosa

Temple, Louise; Sage, Andrew; Schweizer, Herbert P.; Phibbs, Paul V.

doi:10.1007/978-1-4899-0120-0_2

Cited by 34 publications

(39 citation statements)

References 106 publications

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“…5a). In P. aeruginosa, KDPG may physiologically induce repressed genes, including edd, gap and zwf (Temple et al, 1998). However, to our knowledge, there are no data to support this idea.…”

Section: Discussionmentioning

confidence: 85%

Dual regulation of zwf-1 by both 2-keto-3-deoxy-6-phosphogluconate and oxidative stress in Pseudomonas putida

2008

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“…5a). In P. aeruginosa, KDPG may physiologically induce repressed genes, including edd, gap and zwf (Temple et al, 1998). However, to our knowledge, there are no data to support this idea.…”

Section: Discussionmentioning

confidence: 85%

Dual regulation of zwf-1 by both 2-keto-3-deoxy-6-phosphogluconate and oxidative stress in Pseudomonas putida

2008

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“…1) (9,19,36). The members of the pathway that are responsible for the metabolism of glucose to glyceraldehyde 3-phosphate and pyruvate are coordinately regulated and induced by growth on glycerol, fructose, mannitol, glucose, and gluconate.…”

mentioning

confidence: 99%

The Pseudomonas aeruginosa devB/SOL Homolog, pgl , Is a Member of the hex Regulon and Encodes 6-Phosphogluconolactonase

2000

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A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3-phosphate are coordinately regulated, clustered at 39 min on the chromosome, and collectively form the hex regulon. Within the hex cluster is an open reading frame (ORF) with homology to the devB/SOL family of unidentified proteins. This ORF encodes a protein of either 243 or 238 amino acids; it overlaps the 5 end of zwf (encodes glucose-6-phosphate dehydrogenase) and is followed immediately by eda (encodes the Entner-Doudoroff aldolase). The devB/SOL homolog was inactivated in P. aeruginosa PAO1 by recombination with a suicide plasmid containing an interrupted copy of the gene, creating mutant strain PAO8029. PAO8029 grows at 9% of the wild-type rate using mannitol as the carbon source and at 50% of the wild-type rate using gluconate as the carbon source. Cell extracts of PAO8029 were specifically deficient in 6-phosphogluconolactonase (Pgl) activity. The cloned devB/SOL homolog complemented PAO8029 to restore normal growth on mannitol and gluconate and restored Pgl activity. Hence, we have identified this gene as pgl and propose that the devB/SOL family members encode 6-phosphogluconolactonases. Interestingly, three eukaryotic glucose-6-phosphate dehydrogenase (G6PDH) isozymes, from human, rabbit, and Plasmodium falciparum, contain Pgl domains, suggesting that the sequential reactions of G6PDH and Pgl are incorporated in a single protein. 6-Phosphogluconolactonase activity is induced in P. aeruginosa PAO1 by growth on mannitol and repressed by growth on succinate, and it is expressed constitutively in P. aeruginosa PAO8026 (hexR). Taken together, these results establish that Pgl is an essential enzyme of the cyclic Entner-Doudoroff pathway encoded by pgl, a structural gene of the hex regulon.A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates ( Fig. 1) (9,19,36). The members of the pathway that are responsible for the metabolism of glucose to glyceraldehyde 3-phosphate and pyruvate are coordinately regulated and induced by growth on glycerol, fructose, mannitol, glucose, and gluconate. They are clustered in at least three operons near 39 min on the chromosome and are referred to as the hex regulon. They are under the control of the recently identified repressor hexR (29;

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“…Also, P. aeruginosa has no predicted sugar transporters of the major facilitator superfamily (MFS), although E. coli has more than twenty. The apparent lack of sugar transporters in P. aeruginosa correlates with the absence of an intact glycolytic pathway and with its aerobic, oxidative metabolism 18 . b-Oxidative metabolism.…”

Section: Annotation Of P Aeruginosa Genesmentioning

confidence: 99%

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

Stover

Pham

Erwin

et al. 2000

Nature

3,955

2,852

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Carbohydrate Catabolism in Pseudomonas aeruginosa

Cited by 34 publications

References 106 publications

Dual regulation of zwf-1 by both 2-keto-3-deoxy-6-phosphogluconate and oxidative stress in Pseudomonas putida

Dual regulation of zwf-1 by both 2-keto-3-deoxy-6-phosphogluconate and oxidative stress in Pseudomonas putida

The Pseudomonas aeruginosa devB/SOL Homolog, pgl , Is a Member of the hex Regulon and Encodes 6-Phosphogluconolactonase

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen

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