Carbohydrate and amino acid metabolism in fasting and aestivating African lungfish (Protopterus dolloi)

Frick, Natasha Therese; Bystriansky, Jason S.; Ip, Yuen K.; Chew, Shit F.; Ballantyne, James S.

doi:10.1016/j.cbpa.2008.06.003

Cited by 52 publications

(29 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, we note that there are no data regarding mitochondrial substrate levels for C. alboguttata and we cannot rule out the possibility that a particular substrate(s) is used preferentially as an energy source in aestivating skeletal muscle. Whereas respiratory quotient data suggest fatty acids are the preferred substrate for aestivating frogs (van Beurden, 1980), other studies on aestivating animals show that energy may be derived from other sources (carbohydrates, ketone bodies) (Frick et al, 2008a;Frick et al, 2008b ), which were added in conjunction with the complex II substrate succinate (400 μmol l…”

Section: Mitochondrial Respiration and Ros (H 2 O 2 ) Productionmentioning

confidence: 99%

Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse

Reilly

Hickey

Cramp

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

Suppression of disuse-induced muscle atrophy has been associated with altered mitochondrial reactive oxygen species (ROS) production in mammals. However, despite extended hindlimb immobility, aestivating animals exhibit little skeletal muscle atrophy compared with artificially immobilised mammalian models. Therefore, we studied mitochondrial respiration and ROS (H 2 O 2 ) production in permeabilised muscle fibres of the green-striped burrowing frog, Cyclorana alboguttata. Mitochondrial respiration within saponinpermeabilised skeletal and cardiac muscle fibres was measured concurrently with ROS production using high-resolution respirometry coupled to custom-made fluorometers. After 4 months of aestivation, C. alboguttata had significantly depressed whole-body metabolism bỹ 70% relative to control (active) frogs, and mitochondrial respiration in saponin-permeabilised skeletal muscle fibres decreased by almost 50% both in the absence of ADP and during oxidative phosphorylation. Mitochondrial ROS production showed up to an 88% depression in aestivating skeletal muscle when malate, succinate and pyruvate were present at concentrations likely to reflect those in vivo. The percentage ROS released per O 2 molecule consumed was also ~94% less at these concentrations, indicating an intrinsic difference in ROS production capacities during aestivation. We also examined mitochondrial respiration and ROS production in permeabilised cardiac muscle fibres and found that aestivating frogs maintained respiratory flux and ROS production at control levels. These results show that aestivating C. alboguttata has the capacity to independently regulate mitochondrial function in skeletal and cardiac muscles. Furthermore, this work indicates that ROS production can be suppressed in the disused skeletal muscle of aestivating frogs, which may in turn protect against potential oxidative damage and preserve skeletal muscle structure during aestivation and following arousal.

show abstract

Section: Mitochondrial Respiration and Ros (H 2 O 2 ) Productionmentioning

confidence: 99%

Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse

Reilly

Hickey

Cramp

et al. 2013

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…In African lungfish liver, a study has shown an increase in glutamate dehydrogenase (GDH) activity at aestivation, a key enzyme for final step in glutamate biosynthesis. This suggested that a small amount of energy can be gained through increased amino acid catabolism, directed towards the generation of tricarboxylic acid cycle intermediates, which can ultimately use amino acids as fuel stores during aestivation (Frick et al, 2008a). Our analysis of T. pisana transcriptome data (Zhao et al, 2016) has shown that there is GDH present during aestivation ( Figure S3B).…”

Section: Accepted M Manuscriptmentioning

confidence: 97%

“…For instance, aestivated animals are known to store fuel (i.e. amino acids, carbohydrates, lipids or ketone bodies) for energy production (Frick et al, 2008a;Frick et al, 2008b). Also, hibernating mammals store large amounts of lipid, which are primarily used during hibernation exclusively for energy production (Storey 1990).…”

Section: Introductionmentioning

confidence: 99%

Global metabolite analysis of the land snail Theba pisana hemolymph during active and aestivated states

Bose

Centurion

Hodson

et al. 2016

Comparative Biochemistry and Physiology Part D: Genomics and Pr

View full text Add to dashboard Cite

“…Amphibious fishes may be tolerant of some water loss for intervals when they emerse (Horn and Gibson, 1988;Evans et al, 1999), but many have strategies to remain in positive water balance (e.g. Litwiller et al, 2006;Wilkie et al, 2007;Frick et al, 2008a). Behavioural strategies are often used to limit water loss and maintain hydration in fish out of water.…”

Section: Water Availability and Ion Balancementioning

confidence: 99%

Amphibious fishes: evolution and phenotypic plasticity

Wright

Turko

2016

Journal of Experimental Biology

View full text Add to dashboard Cite

Amphibious fishes spend part of their life in terrestrial habitats. The ability to tolerate life on land has evolved independently many times, with more than 200 extant species of amphibious fishes spanning 17 orders now reported. Many adaptations for life out of water have been described in the literature, and adaptive phenotypic plasticity may play an equally important role in promoting favourable matches between the terrestrial habitat and behavioural, physiological, biochemical and morphological characteristics. Amphibious fishes living at the interface of two very different environments must respond to issues relating to buoyancy/gravity, hydration/ desiccation, low/high O 2 availability, low/high CO 2 accumulation and high/low NH 3 solubility each time they traverse the air-water interface. Here, we review the literature for examples of plastic traits associated with the response to each of these challenges. Because there is evidence that phenotypic plasticity can facilitate the evolution of fixed traits in general, we summarize the types of investigations needed to more fully determine whether plasticity in extant amphibious fishes can provide indications of the strategies used during the evolution of terrestriality in tetrapods.

show abstract

Carbohydrate and amino acid metabolism in fasting and aestivating African lungfish (Protopterus dolloi)

Cited by 52 publications

References 45 publications

Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse

Decreased hydrogen peroxide production and mitochondrial respiration in skeletal muscle but not cardiac muscle of the green-striped burrowing frog, a natural model of muscle disuse

Global metabolite analysis of the land snail Theba pisana hemolymph during active and aestivated states

Amphibious fishes: evolution and phenotypic plasticity

Contact Info

Product

Resources

About