Carbohydrate analysis with ion chromatography using Eurokat stationary phases

Oberdorfer, Franz; Kemper, Katharina; Kaleja, Michael; Reusch, Joachim; Gottschall, Klaus

doi:10.1016/s0021-9673(01)95964-3

Cited by 13 publications

(1 citation statement)

References 2 publications

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“…2 The medical product should be analysed, for example by thin-layer chromatography (TLC), for the toxic contaminant 4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo- [8,8,8]hexacosane (Kryptofix 222) which is used as a catalyst for nucleophilic fluorination of 1,3,4,6-tetra-O-acetyl-2-Otrifluoromethanesulfonyl-β-D-mannopyranose (mannose triflate). 3 The enantiomers, 2-fluoro-2-deoxy-D-glucose, 2fluoro-2-deoxy-D-mannose and 2-fluoro-2-deoxy-Dgalactose have been resolved by HPLC on cation exchange resin in the Ca 2+ or Pb 2+ form, 4 but refractometric detection is not very sensitive and the salt solutions used are not suitable for an atmospheric-pressure ionization (API) mass-spectrometric detector (MSD) analysis. However, amino-column chromatography provides a rugged sugar analysis using acetonitrile + water eluants.…”

Section: Introductionmentioning

confidence: 99%

Atmospheric Pressure Electrospray Ionization Mass Spectra of Glucose and 2-Fluorodeoxyglucose for Quantitative Analysis of 2-Fluorodeoxyglucose by High-Performance Liquid Chromatography

Macášek

Brúder

Patakyová

et al. 2003

Eur J Mass Spectrom (Chichester)

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2-Fluoro-2-deoxy-D-glucose (FDG) labeled by fluorine-18 is the most widely used radiopharmaceutical for positron emission tomography (PET). For high-performance liquid chromatography (HPLC)/MS assay and quality control, the mass spectra of FDG and glucose (Glc) in organic + water solutions were studied by flow injection analysis (FIA) and in a chromatographic eluate. In acetonitrile (MeCN) + 0.025% ammonium formate (NH(4)HCO(2)) solvent (80 : 20), electrospray ionisation (ESI) of glucose-FDG provides M.NH(4)(+) and 2M.Na(+) (M = Glc or FDG) as the most intense positive ions. Formation of the latter ions and also of M.MeCN.Na(+) and 2MeCN. Na(+) is typical of the presence of NaCl in the ESI inlet. The positive ions include heavier ions corresponding to the impurities separated by HPLC and also to the cross-ring fragmentation of complexes (2FDG. aMeCNX)L, where a = 0 or 1, L is either Na(+) or NH(4)(+) and X is a fragmented pyranose or anhydropyranose residue. The second most abundant Glc negative ion is m/z = 359 which was interpreted as (2GlcH(+))(). The negative-ion spectrum of FDG has dominating lines due to FDG.HCO(2)() ions at m/z 227 and also (2FDGH(+))() at m/z 363. The m/z 363 signal is suppressed in the presence of NaCl at a molar ratio of 4 : 1 to NH(4)HCO(2), while the ions at m/z 217 and 219, i.e. FDG.Cl(), become three times more intense than FDG.HCO(2)(). The latter ion appears to be most suitable as an analytical signal for chemical analysis of FDG at m/z 226 and 227. Limits of FDG quantitation (LOQ) of 19 ng and 21 ng were found for the 200(+) and 227() ion signals, respectively, and are wholly adequate for verification of total FDG content in radiopharmaceuticals.

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Section: Introductionmentioning

confidence: 99%

Atmospheric Pressure Electrospray Ionization Mass Spectra of Glucose and 2-Fluorodeoxyglucose for Quantitative Analysis of 2-Fluorodeoxyglucose by High-Performance Liquid Chromatography

Macášek

Brúder

Patakyová

et al. 2003

Eur J Mass Spectrom (Chichester)

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Combination treatment based on metabolic effects of dinaline

Schaider

Haberkorn

Petru

et al. 1995

J Cancer Res Clin Oncol

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The human colon carcinoma cell line SW 707 was exposed for up to 72 h to the new antineoplastic agent 4-amino-N-(2'-aminophenyl)benzamide (GOE 1734, dinaline). Thereafter, uptake measurements with fluorodeoxy-[14C]glucose (FdGlc) were performed and cell-cycle fractions as well as adenine nucleotide pools were determined by flow cytometry and HPLC. One day after a 24 h exposure to 20-540 microM dinaline a 2.0-to 2.5-fold enhancement of FdGlc uptake was observed, and the values after 48-h or 72-h incubations showed a 2.5- to 3.5-fold or a 2.0-fold increase respectively. For all periods of exposure a diminished S phase (3%-71% of control) was found initially after incubation, demonstrating the antiproliferative effect of dinaline, with total recovery after 1 day. Adenine nucleotide pools were not diminished concomitantly. The enhanced FdGlc uptake caused by dinaline was the basis for choosing 2-deoxyglucose (dGlc) as the combination partner, which acts as an antimetabolite to enzymes involved in glucose metabolism. Several combinations of dinaline and dGlc were analyzed for their effects on growth inhibition. Almost 50% additional decrease in cell number as compared to monotherapy with dinaline was found after coexposure to 12 mM dGlc and 20 microM dinaline 24 h after incubation. Similar effects were observed 2 days after incubation with the two drugs. After 3 days, the cell numbers reached monotherapy levels. Since the cytostatic effect of dinaline could be enhanced by dGlc although incubation with dGlc alone caused no changes in cell number, the combined effect of both agents is synergistic. These results imply that dinaline might have applications in combination treatment in vivo.

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Application of α-aminoisobutyric acid,l-methionine, thymidine and 2-fluoro-2-deoxy-d-glucose to monitor effects of chemotherapy in a human colon carcinoma cell line

et al. 1996

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Up to 4 h after treatment of human SW 707 colon carcinoma cells with the antineoplastic drug 4-amino-N-(2'-aminophenyl)-benzamide (GOE 1734, dinaline), the effects of tumour cell metabolism and proliferation were examined in vitro. Four tracers which can be labelled with isotopes suitable for positron emission tomography (PET) were used for this purpose: alpha-aminoisobutyric acid (AIB) and methionine to study changes in amino acid transport and protein synthesis, thymidine to observe changes in tumour proliferation and 2-fluoro-2-deoxy-D-glucose (FDG) to estimate glucose metabolism. Dinaline showed an inhibition of the sodium-dependent and -independent uptake of AIB. The methionine uptake was found to increase shortly after therapy. Thymidine incorporation into DNA was impaired and the FDG uptake showed a maximally 2.2-fold enhancement. Inhibition of AIB uptake suggests changes in amino acid transport, whereas increased uptake of methionine and FDG points to an enhancement of protein synthesis and glycolysis caused by repair mechanisms. The cytostatic and antiproliferative effect of dinaline, observed in cell growth curves, could be demonstrated by the impaired thymidine incorporation into DNA. This study demonstrates that in vitro screening with radiotracers suitable for PET can help to clarify effects of new antineoplastic substances on tumour cell metabolism. These data may be applied to choose the appropriate time schedule for monitoring therapeutic effects on tumour tissue.

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Carbohydrate analysis with ion chromatography using Eurokat stationary phases

Cited by 13 publications

References 2 publications

Atmospheric Pressure Electrospray Ionization Mass Spectra of Glucose and 2-Fluorodeoxyglucose for Quantitative Analysis of 2-Fluorodeoxyglucose by High-Performance Liquid Chromatography

Atmospheric Pressure Electrospray Ionization Mass Spectra of Glucose and 2-Fluorodeoxyglucose for Quantitative Analysis of 2-Fluorodeoxyglucose by High-Performance Liquid Chromatography

Combination treatment based on metabolic effects of dinaline

Application of α-aminoisobutyric acid,l-methionine, thymidine and 2-fluoro-2-deoxy-d-glucose to monitor effects of chemotherapy in a human colon carcinoma cell line

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