Carbohydrase hydrolysis of canola to enhance oil extraction with hexane

Sosulski, K.; Sosulski, F. W.; Coxworth, E.

doi:10.1007/bf02663076

Cited by 70 publications

(51 citation statements)

References 2 publications

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“…enzyme untreated kernel. Such increased oil recovery has also been reported by Sosulski et al (1988) and Sarkar et al (1998). The increased oil recovery ranged from 0.14 to 2.53%.…”

Section: Increased Oil Recoverysupporting

confidence: 82%

“…The flasks were then incubated at 45°C for desired experimental period of incubation. After incubation, the samples were dried on the petridishes at 70°C to inactivate the enzyme (Sosulski et al 1988 andSarkar et al 1998) and also to reduce moisture content to 3-6% (w.b.) desired for further extraction for oil.…”

Section: Methodsmentioning

confidence: 99%

“…Increase in extractable oil may be attributed to the action of crude enzymes on ground wild apricot kernel. Enzymes enhance hydrolysis of the linkages in lipopolysaccharides, lipo-proteins and some other bound compounds, rendering easier extraction and therefore improvement in oil recovery (Fullbrook 1983;Sosulski et al 1988).…”

Section: Increased Oil Recoverymentioning

confidence: 99%

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Optimization of parameters for enhanced oil recovery from enzyme treated wild apricot kernels

et al. 2011

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Present investigation was undertaken with the overall objective of optimizing the enzymatic parameters i.e. moisture content during hydrolysis, enzyme concentration, enzyme ratio and incubation period on wild apricot kernel processing for better oil extractability and increased oil recovery. Response surface methodology was adopted in the experimental design. A central composite rotatable design of four variables at five levels was chosen. The parameters and their range for the experiments were moisture content during hydrolysis (20-32%, w.b.), enzyme concentration (12-16% v/w of sample), combination of pectolytic and cellulolytic enzyme i.e. enzyme ratio (30:70-70:30) and incubation period (12-16 h). Aspergillus foetidus and Trichoderma viride was used for production of crude enzyme i.e. pectolytic and cellulolytic enzyme respectively. A complete second order model for increased oil recovery as the function of enzymatic parameters fitted the data well. The best fit model for oil recovery was also developed. The effect of various parameters on increased oil recovery was determined at linear, quadric and interaction level. The increased oil recovery ranged from 0.14 to 2.53%. The corresponding conditions for maximum oil recovery were 23% (w.b.), 15 v/w of the sample, 60:40 (pectolytic: cellulolytic), 13 h. Results of the study indicated that incubation period during enzymatic hydrolysis is the most important factor affecting oil yield followed by enzyme ratio, moisture content and enzyme concentration in the decreasing order. Enzyme ratio, incubation period and moisture content had insignificant effect on oil recovery. Second order model for increased oil recovery as a function of enzymatic hydrolysis parameters predicted the data adequately.

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“…enzyme untreated kernel. Such increased oil recovery has also been reported by Sosulski et al (1988) and Sarkar et al (1998). The increased oil recovery ranged from 0.14 to 2.53%.…”

Section: Increased Oil Recoverysupporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

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Optimization of parameters for enhanced oil recovery from enzyme treated wild apricot kernels

et al. 2011

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“…Improvement of the extractability of vegetable oils from enzymatically treated fruits and seeds, and optimization of hydrolysis time and characterization of resulting products have been reported for olives (Alba Mendoza et al 1990), coconut (Cintra MacGlone et al 1986), rape seed (Sousulski et al 1988;Sarkar et al 1998), and soybean (Fullbrook 1983). Enzymatic hydrolysis has been shown to be a useful option for pre-treatment.…”

mentioning

confidence: 99%

“…Enhanced oil recovery in enzyme aided extraction has been suggested to be due to the bio-degradation of cell walls and hydrolysis of complex lipoproteins and lipopolysaccharides into simpler molecules releasing extra oil which was otherwise non-extractable (Srivastava et al 2004). Enzymes to enhance extractability of the oilseeds vary in efficiency, but formulations with mixed activities have proved to be very effective (Sousulski et al 1988;Dominguez et al 1994;Sharma et al 2007). The use of enzyme significantly improve the oil recovery and had no adverse effect on the nutritive and pharmaceutical quality of the oil and it was more resistant to auto oxidation as observed by sensory score (Sharma et al 2007).…”

mentioning

confidence: 99%

Improvement of efficiency of oil extraction from wild apricot kernels by using enzymes

et al. 2013

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An experiment was conducted to evaluate and standardize the protocol for enhancing recovery of oil and quality from cold pressed wild apricot kernels by using various enzymes. Wild apricot kernels were ground into powder in a grinder. Different lots of 3 kg powdered kernel were prepared and treated with different concentrations of enzyme solutions viz. Pectazyme (Pectinase), Mashzyme (Cellulase) and Pectazyme + Mashzyme. Kernel powder mixed with enzyme solutions were kept for 2 h at 50(±2)°C temperature for enzymatic treatment before its use for oil extraction through oil expeller. Results indicate that use of enzymes resulted in enhancement of oil recovery by 9.00-14.22 %. Maximum oil recovery was observed at 0.3-0.4 % enzyme concentration for both the enzymes individually, as well as in combination. All the three enzymatic treatments resulted in increasing oil yield. However, with 0.3 % (Pectazyme + Mashzyme) combination, maximum oil recovery of 47.33 % could be observed against were 33.11 % in control. The oil content left (wasted) in the cake and residue were reduced from 11.67 and 11.60 % to 7.31 and 2.72 % respectively, thus showing a high increase in efficiency of oil recovery from wild apricot kernels. Quality characteristics indicate that the oil quality was not adversely affected by enzymatic treatment. It was concluded treatment of powdered wild apricot kernels with 0.3 % (Pectazyme + Mashzyme) combination was highly effective in increasing oil recovery by 14.22 % without adversely affecting the quality and thus may be commercially used by the industry for reducing wastage of highly precious oil in the cake.

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Aqueous Extraction of Coconut Oil by an Enzyme-Assisted Process

Tano‐Debrah

Ohta

1997

J. Sci. Food Agric.

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A pre-extraction enzyme treatment of copra meal was incorporated into a rural wet copra oil extraction process to investigate the possibility of improving the traditional process by the enzyme treatment. The enzymes used in the study were combinations of a protease from Aspergillus niger, a cellulase/ hemicellulase preparation from T richoderma reseei, and a-amylase from A oryzae and a pectinase from A niger (all were crude preparations). Finely milled copra meal samples were mixed with water and the enzymes and incubated at 37¡C for 6 h or more. After the treatment, the meal was extracted using a water Ñotation technique. Yield increases of about 50%, relative to experimental controls, were observed, suggesting the possibility of using the enzyme-assisted oil extraction methods to improve upon traditional copra processing.

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Carbohydrase hydrolysis of canola to enhance oil extraction with hexane

Cited by 70 publications

References 2 publications

Optimization of parameters for enhanced oil recovery from enzyme treated wild apricot kernels

Optimization of parameters for enhanced oil recovery from enzyme treated wild apricot kernels

Improvement of efficiency of oil extraction from wild apricot kernels by using enzymes

Aqueous Extraction of Coconut Oil by an Enzyme-Assisted Process

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