Carbofuran-induced biochemical changes inClarias batrachus

Abstract

:The e †ect of carbofuran, an organo-carbamate pesticide, upon the level of protein as well as the activity of lactate dehydrogenase (EC 1.1.1.28, LDH) was studied by exposing the teleost Ðsh, Clarias batrachus, to di †erent subacute concentrations (0É01 and 0É02 mg litre~1) for 96 h and 15 days. The results showed a drastic decrease in the protein content in di †erent body organs of the Ðsh. The pesticide also caused a signiÐcant decrease in the level of activity of LDH in di †erent body tissues of the Ðsh, the e †ect being more pronounced in the gills, muscle, brain and liver than in kidney and heart. The decrease in protein content and the activity of LDH in Ðsh tissues was more marked at the higher concentration of the pesticide for the longer duration of treatment. The results suggested that carbofuran has an e †ect at very low concentration (compared to its LC 50 value) possibly at the level of protein metabolism, and also inhibits the activity of LDH, the terminal glycolytic enzyme.1998 SCI ( Pestic. Sci., 53, 285È290 (1998)

Effects of lead nitrate on the activity of metabolic enzymes during early developmental stages of the African catfish, Clarias gariepinus (Burchell, 1822)

“…Sastry and Gupta (1980) postulated that inhibition of activity may be due to (1) ion imbalance or to (2) intracellular action of metal subsequent to initial plasma membrane damage. This inhibitory effect may also be due to the direct binding of the lead to the enzyme (Rajanna et al 1990) and the formation of an enzyme-inhibitor complex (Singh and Sharma 1998). In consistence with our results, Mishra and Shukla (1997) reported that endosulfan (an insecticide) inhibited the activity of LDH in muscles and liver of the catfish Clarias batrachus.…”

“…The increase in G6PDH activity represents protection against elevated levels of ROS in cells exposed to an oxidant stress through the increased production of NADH (Stephensen et al 2000;Leopold et al 2003) LDH is generally associated with cellular metabolic activity. Such activity is inhibited under stress, especially after exposure to heavy metals (Singh et al 1974;Sastry and Gupta 1980;Gupta and Sastry 1981;Sastry and Rao 1984;Mishra and Shukla 1997;Fennouh et al 1998;Singh and Sharma 1998;Almeida et al 2001;Elumalai et al 2002). In C. gariepinus at 30 h-PFS, the 100 lg/l lead dose led to a significant increase in LDH enzyme activity.…”

“…It has been used for demonstrating tissue damage in fish (Das et al 2004a). In most cases of tissue damage, whether due to disease or toxic compound, the activity of LDH was reported to be significantly affected (Singh and Sharma 1998). LDH is a source of the oxidised coenzyme during the period of transient anaerobiosis or a reduced form of such coenzyme during aerobiosis (Coppes 1992).…”

Protective effects of perennial herb, against furadan 3G induced cytotoxicity in Melissa officinalis Channa punctatus

“…Pesticides generally affect biologically active molecules ., proteins, carbohydrates and lipids (Singh and Sharma, 1998). In the present investigation, alteration in enzyme level of liver tissues in group 2 animals indicates that a higher activity of liver enzymes is required to detoxify the toxicants introduced in the body under furadan 3G exposure.…”

Changes in nutritional parameters in diploid and triploid African catfish Clarias gariepinus following chlorpyrifos exposure

“…Chemical pesticides used in agriculture impact the activity of biologically active molecules such as proteins, lipids, and carbohydrates (Singh & Sharma 1998). Protein plays a key role in metabolic pathways (Suryavanshi et al 2009) and in improving the health, growth, development, and disease resistance of organisms (Xu et al 2016).…”