“…[5], thin-layer chromatography-(TLC-) densitometry [1,6], and high-performance thin-layer chromatography (HPTLC) [7] have been used for the estimation of hydrocortisone in pharmaceutical formulations. However, modern chromatographic techniques such as high-performance liquid chromatography (HPLC) [8-12] and ultraperformance liquid chromatography (UPLC) [13] are preferentially employed for the determination of hydrocortisone in conventional pharmaceutical products such as tablets, creams, and injections.…”