In the January issue of this Journal, Dr. W. L. Beebe described a method for disinfecting books by carbo-gasoline. He recommended that books be immersed for twenty minutes in a 2 per cent. solution of carbolic acid in 88 Baum6 gasoline. He made 65 inoculations using bouillon cultures and obtained growth in only one case.In order to test this method I autoclaved several books, dried them in a sterile chamber, and inoculated them twenty pages apart, with loopfuls of agar, or bouillon cultures. The books were again placed in a sterile chamber, for forty-eight hours, that the cultures might thoroughly dry, in order to prevent danger of the organisms being washed off. They were then left in 880 Bauim6 gasoline saturated with carbolic acid (about a 2 per cent. solution) for one hour and then dried for ninety-six hours to let all the gasoline evaporate before cultures were made. Eighty inoculations were made; twenty-five of B. coli communis, twenty-seven of Staphylococcus pyogenes aureus, and twenty-eight of B. diphtheriae. Fifty-seven of these grew; fifteen of B. coli, seventeen of Staphylococcus pyogenes aureus, and twentyfive of the B. diphtheriae.In order to determine whether saturated carbo-gasoline will kill bacteria when it is in direct contact with them, three test tube cultures each of B. coli communis, Staphylococcus pyogenes aureus, and B. diphtheriae were filled with carbo-gasoline. This was drawn off after one hour and ten minutes, and ninety-six hours later cultures were made. Growth occurred in two of the B. coli test tubes, in two of the tubes of Staphylococcus pyogenes aureus and in one of the diphtheria tubes.The lack of growth in Beebe's experiments was probably due to his bouillon cultures being washed off by the gasoline, since two of his books were only dried one hour and the third not at all. In my own experiments, although the books were dried for forty-eight hours, growth occurred in 80 per cent. of the agar and 25 per cent. of the bouillon cultures.*These experiments were made in the biological laboratory of Clark University, Worcester, Mass.