Carbaryl InducesCYP1A1Gene Expression in HepG2 and HaCaT Cells but Is Not a Ligand of the Human Hepatic Ah Receptor

Ledirac, Nathalie; Delescluse, C; Sousa, Georges de; Pralavorio, M.; Lesca, P.; Amichot, Marcel; Bergé, Jean-Baptiste; Rahmani, Roger

doi:10.1006/taap.1997.8120

Cited by 63 publications

(34 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous compounds have been reported to induce Cyp1a1 that do not seem to compete with TCDD for binding to the AhR, including thiazolium compounds, retinoids, carotenoids, benzimidazoles, carbamates, and aminoquinoline (Daujat et al, 1992;Aix et al, 1994;Lesca et al, 1995;Gradelet et al, 1997;Ledirac et al, 1997;Fontaine et al, 1999). Although the lack of in vitro AhR binding for the many Cyp1a1 inducers may result from technical limitations of the binding assays Denison and Nagy, 2003), it has also been suggested that many of these com- pounds may induce Cyp1a1 through multiple modes of indirect AhR activation.…”

Section: Discussionmentioning

confidence: 99%

Induction of Cyp1a1 Is a Nonspecific Biomarker of Aryl Hydrocarbon Receptor Activation: Results of Large Scale Screening of Pharmaceuticals and Toxicants in Vivo and in Vitro

Hu¹,

et al. 2007

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Induction of Cyp1a1 Is a Nonspecific Biomarker of Aryl Hydrocarbon Receptor Activation: Results of Large Scale Screening of Pharmaceuticals and Toxicants in Vivo and in Vitro

Hu¹,

et al. 2007

View full text Add to dashboard Cite

“…59) Long et al 60) reported that 8 of 23 pesticides currently used in Denmark revealed AhR-mediated activity in human and rat hepatoma cell lines. In addition, a carbamate-type insecticide, carbaryl, 61,62) and a urea-type herbicide, diuron, 63,64) were found to act as AhR agonists in AhR-mediated reporter gene assays using mammalian hepatoma cells or recombinant yeast cells.…”

Section: Effects Of Pesticides Via Ahrmentioning

confidence: 99%

Endocrine-disrupting Potential of Pesticides via Nuclear Receptors and Aryl Hydrocarbon Receptor

Kojima

Takeuchi

Nagai

2010

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

Nuclear receptors (NRs) and the aryl hydrocarbon receptor (AhR) form a ligand-dependent transcription factor that regulates the genes involved in key physiological functions such as cell growth and differentiation, development, homeostasis, and metabolism. These receptors are potential targets of endocrine-disrupting chemicals (EDCs). To date, many studies have shown that EDCs, such as plasticizers, pesticides, and dioxins, can function as ligands of NRs and AhR. In this review, we focus on recent studies showing that a variety of pesticides, intentionally released into the environment, have agonistic and/or antagonistic activity against NRs and AhR, and present our transactivation assay-based screening results for 200 pesticides against estrogen receptors (ERs), androgen receptor (AR), thyroid hormone receptors (TRs), pregnane X receptor (PXR), peroxisome proliferator-activated receptors (PPARs), and AhR. Our studies have shown that a number of pesticides possess ERα, ERβ, and PXR agonistic activity as well as AR antagonistic activity, whereas none of the pesticides affect the TRα 1 , TRβ 1 , and PPARγ-mediated signaling pathways. In addition, several of the 200 tested pesticides were found to have PPARα and AhR agonistic, and ERα and ERβ antagonistic activity. Although the activities of each of these compounds were weak compared to those of endogenous hormone or dioxins, the endocrine-disrupting potential of pesticides, particularly those which function against ERα/β, AR, and PXR, may reflect that of numerous environmental chemicals.

show abstract

“…In this experiment, since the mouse Hepa-1 cell is not sensitive to omeprazole, we need to confirm the presence in these cells of a signal transduction pathway activating the Ah receptor without ligand binding (as in human HepG2 cells). In fact, in human HepG2 cells, many chemicals have been reported to activate the Ah receptor ligand-independently (Aix et al, 1994;Curi-Pedrosa et al, 1994;Jeong et al, 1997;Ledirac et al, 1997). However, in mouse Hepa-1 cells, ·-NF is the only candidate.…”

Section: Alpha-nf-sensitivity Gene In the Mouse Is The Counterpart Ofmentioning

confidence: 99%

Presence on human chromosome 10 of omeprazole-sensitivity gene whose product mediates CYP1A1 induction

Kikuchi

Fukushige²,

Shibazaki³

et al. 2002

Cytogenet Genome Res

View full text Add to dashboard Cite

Previously, we showed that CYP1A1 expression can be induced by omeprazole (OP) in the human cell line HepG2, but not in the mouse cell line Hepa-1. Now we show induction of CYP1A1 by α-naphthoflavone (αNF) in Hepa-1 cells. This induction was inhibited by the tyrosine kinase inhibitor herbimycin A, but not by the aromatic hydrocarbon (Ah)-receptor antagonist PD98059, suggesting the presence of a ligand-independent signal-transduction pathway in the mouse cell line too. We utilized the lack of CYP1A1 induction by OP in Hepa-1 cells to map a putative human gene for OP-respon- siveness in cell hybrids produced by fusion of Hepa-1 and HepG2 cells. OP-induced CYP1A1 expression was detected in four out of the 32 Hepa-1 × HepG2 cell hybrids analyzed. To help identify the gene locus, a radiation-hybrid cell (E11) was constructed. Use of reverse-fluorescence in situ hybridization revealed that these five cell lines commonly retained human chromosome 10p. These results suggest that the human gene for OP-responsiveness is present on chromosome 10p.

show abstract

Carbaryl InducesCYP1A1Gene Expression in HepG2 and HaCaT Cells but Is Not a Ligand of the Human Hepatic Ah Receptor

Cited by 63 publications

References 24 publications

Induction of Cyp1a1 Is a Nonspecific Biomarker of Aryl Hydrocarbon Receptor Activation: Results of Large Scale Screening of Pharmaceuticals and Toxicants in Vivo and in Vitro

Induction of Cyp1a1 Is a Nonspecific Biomarker of Aryl Hydrocarbon Receptor Activation: Results of Large Scale Screening of Pharmaceuticals and Toxicants in Vivo and in Vitro

Endocrine-disrupting Potential of Pesticides via Nuclear Receptors and Aryl Hydrocarbon Receptor

Presence on human chromosome 10 of omeprazole-sensitivity gene whose product mediates CYP1A1 induction

Contact Info

Product

Resources

About