Carbaryl-induced thyroid dysfunction in the freshwater catfish Clarias batrachus

Sinha, Nivedita; Lal, Bechan; Singh, T. N.

doi:10.1016/0147-6513(91)90062-t

Cited by 27 publications

(14 citation statements)

References 16 publications

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“…This was accomplished initially by injecting *I-and then determining the identities of the radioiodinated products chromatographically, using paper (Jacoby and Hickman, 1966) or thin-layer (Osborn and Simpson, 1969a) methods. More recently, the levels of TH in extracts of thyroid hydrolysates have been measured by immunoassay (Lahti et al, 1985a;Sinha et al, 1991a, Byamungu et aL, 1992. In combination these studies demonstrate that in addition to T 4 the TG comprises small or negligible quantities of T 3 but significant amounts of monoiodotyrosine (MIT) and diiodotyrosine (DIT).…”

Section: Hormone Biosynthesismentioning

confidence: 99%

“…Some form of iodoperoxidase activity occurs in teleosts (Nath and Bhattacharya, 1976), which shows either direct or indirect sensitivity to TSH and a variety of pollutants, indicating its value as a discriminatory index of change at the level of the thyroid tissue (De and Bhattacharya, 1976;Chatterjee and Battacharya, 1985;Chakraborti and Bhattacharya, 1978;Yadav and Singh, 1987a,b;Bhattacharya et al, 1989;Sinha et al, 1991aSinha et al, , 1992. Because iodoperoxidase activity is involved in such critical steps in TH biosynthesis, its activity is probably a reliable indicator of the rate of TH biosynthesis.…”

Section: Hormone Biosynthesismentioning

confidence: 99%

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Measurement and regulation of thyroidal status in teleost fish

Eales

Brown

1993

Rev Fish Biol Fisheries

203

132

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Section: Hormone Biosynthesismentioning

confidence: 99%

Section: Hormone Biosynthesismentioning

confidence: 99%

Measurement and regulation of thyroidal status in teleost fish

Eales

Brown

1993

Rev Fish Biol Fisheries

203

132

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“…Both imidacloprid and mancozeb could lead to an impairment of HPT axis in preparatory and breeding phases and lead to distinct alterations in weight, volume, and histopathology of thyroid gland (Pandey and Mohanty, 2015). Thyroid dysfunction and changes in circulating THs were found in the freshwater catfish induced by carbaryl (Sinha et al, 1991). Surprisingly, among these tested pesticides, triadimefon and strobilurin fungicides azoxystrobin, kresoxim-methyl, pyraclostrobine displayed antagonistic effects at high concentration and showed agonistic activity at low concentration in the luciferase reporter gene assay.…”

Section: Discussionmentioning

confidence: 97%

Editor’s Highlight: Structure-Based Investigation on the Binding and Activation of Typical Pesticides With Thyroid Receptor

Xiang

Han

Yao

et al. 2017

Toxicological Sciences

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A broad range of pesticides have been reported to interfere with the normal function of the thyroid endocrine system. However, the precise mechanism(s) of action has not yet been thoroughly elucidated. In this study, 21 pesticides were assessed for their binding interactions and the potential to disrupt thyroid homeostasis. In the GH3 luciferase reporter gene assays, 5 of the pesticides tested had agonistic effects in the order of procymidone > imidacloprid > mancozeb > fluroxypyr > atrazine. 11 pesticides inhibited luciferase activity of T3 to varying degrees, demonstrating their antagonistic activity. And there are 4 pesticides showed mixed effects when treated with different concentrations. Surface plasmon resonance (SPR) biosensor technique was used to directly measure the binding interactions of these pesticides to the human thyroid hormone receptor (hTR). 13 pesticides were observed to bind directly with TR, with a KD ranging from 4.80E-08 M to 9.44E-07 M. The association and disassociation of the hTR/pesticide complex revealed 2 distinctive binding modes between the agonists and antagonists. At the same time, a different binding mode was displayed by the pesticides showed mix agonist and antagonist activity. In addition, the molecular docking simulation analyses indicated that the interaction energy calculated by CDOCKER for the agonists and antagonists correlated well with the KD values measured by the surface plasmon resonance assay. These results help to explain the differences of the TR activities of these tested pesticides.

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“…The toxicity of carbaryl to fish is species-specific, high to slight to freshwater fish species, and moderate to ocean and estuary fish species on an acute basis (Beyers et al, 1994;McKim et al, 1987;Sinha et al, 1991). Salmon, trout, and perch are reported as the most sensitive species, which will die when the concentration of carbaryl varies between 250 and 970 ng mL −1 (USEPA, 2002).…”

Section: Introductionmentioning

confidence: 98%

Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

Pandey

Guo

2015

J. Ocean Univ. China

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In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill (FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking (NRU), lactate dehydrogenase (LDH) releasing and Hoechst 33342 and propidium idodide (PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC 50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L −1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl (20 mg L −1 , P > 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L −1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time-and concentration-dependent manners at specific stages. The 24 h-, 48 h-and 96 h-LC 50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L −1 , respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC 50 and LC 50 indicated.

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Carbaryl-induced thyroid dysfunction in the freshwater catfish Clarias batrachus

Cited by 27 publications

References 16 publications

Measurement and regulation of thyroidal status in teleost fish

Measurement and regulation of thyroidal status in teleost fish

Editor’s Highlight: Structure-Based Investigation on the Binding and Activation of Typical Pesticides With Thyroid Receptor

Evaluation of cytotoxicity and genotoxicity of insecticide carbaryl to flounder gill cells and its teratogenicity to zebrafish embryos

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