Carbapenem and Colistin Resistance, Integrons and Plasmid Replicon Types in Multi-Drug Resistant Klebsiella Strains Isolated in Turkey

Kalaycı-Yüksek, Fatma; Gümüş, Defne; Uyanik-Ocal, Aysun; Gün, Gökhan; Bayirli-Turan, Derya; Macunluoglu, Asli-Ceren; Nakipoğlu, Yaşar; Anğ-Küçüker, Mine

doi:10.7754/clin.lab.2022.220503

Cited by 2 publications

(3 citation statements)

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“…Changes in NDM rates by years were not found statistically significant. In two different studies conducted in our hospital in the following years, NDM rates were found to be 36% and 27.7% (24,25). Our NDM-type carbapenemase ratio in these two studies seems to be consistent with the data from 2017.…”

Section: Discussionsupporting

confidence: 86%

“…In a prospective, multicentre observational cohort study conducted in Turkey in 2018-2019, the association of OXA-48-like and NDM was found to be 16% (30). In two studies conducted in our hospital in 2021 and 2021-2022, this rate was found to be 36% and 27.7% (24,25). The co-existence of OXA and NDM was found in five (5.7%) strains in our study.…”

Section: Discussionsupporting

confidence: 51%

“…The decrease in the last two years was found insignificant. However, in two different studies conducted in our hospital in the following years, OXA-type carbapenemase rates were found to be 16% and 25.8% (24,25). It is seen that OXA-type carbapenemase rates continued to decrease in the following years.…”

Section: Discussionmentioning

confidence: 83%

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Detection of Carbapenem Resistance Using the Genotypic and Phenotypic Methods in Klebsiella pneumoniae

Durmuş,

Aydin

2024

Düzce Tıp Fakültesi Dergisi

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Aim: This study aimed to detect the carbapenem resistance of the Klebsiella pneumoniae strains, isolated from clinical specimens with genotypic and phenotypic methods. Material and Methods: A total of 87 Klebsiella pneumoniae strains whose carbapenem resistance was determined by disc diffusion method were included in the study. Carbapenemase was investigated using the combined disk method and polymerase chain reaction (PCR). Results: The evaluation of the PCR results demonstrated that OXA was detected in 60 (68.9%) samples, NDM was detected in 20 (22.9%), OXA + NDM in 5 (5.7%), and KPC was detected in 1 (1.1%) out of 87 clinical samples. Carbapenemase was not detected in one specimen with the PCR method. The results were found compatible with the combined disc test results for all isolates which were detected as only OXA, NDM, and KPC type carbapenemase positive. In 5 (5.7%) strains in which the co-existence of NDM and OXA type carbapenemases was detected by PCR, the combined disc method detected only OXA type carbapenemase. Conclusion: The combined disk method is inadequate in the presence of strains that have multiple carbapenemases, and also have OXA which is the most frequently detected carbapenemase in our hospital. EUCAST recommends verification by other methods in the presence of OXA-48. Genotypic methods can be used for confirmation testing. The detections of strains with NDM, multiple carbapenemases, and the first detection of KPC were striking in the study. Monitoring the spread of these strains in the hospital will be necessary for infection control.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 51%

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Detection of Carbapenem Resistance Using the Genotypic and Phenotypic Methods in Klebsiella pneumoniae

Durmuş,

Aydin

2024

Düzce Tıp Fakültesi Dergisi

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Characterization of resistance genes and replicon typing in Carbapenem-resistant Klebsiella pneumoniae strains

Karaman,

Çiçek,

Şemen

et al. 2024

Ann Clin Microbiol Antimicrob

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Objective In our study, K. pneumoniae strains (non-susceptible to carbapenem) (n = 60) were obtained from various clinical samples from Rize State Hospital between 2015 and 2017 and it is aimed to identify antibiotic resistance genes and replicon typing. Methods Antibiotic susceptibility tests of the strains were performed with Kirby-Bauer disk diffusion test and the Vitek-2 automated system (BioMerieux, France). Antibiotic resistance genes and replicon typing was characterized by PCR method. Results It was determined that K. pneumaniae isolates were mostly isolated from the samples of the intensive care unit. All of the K. pneumoniae strains examined in this study were found to be ampicillin/sulbactam and ertapenem resistant but colistin susceptible. Amoxacillin/clavulonic acid resistance was detected at 98.14% of strains. The blaOXA-48 gene was mostly detected in isolates. The most common type of plasmid was I1 and 3 different plasmid types were found in five different strains together. Conclusion This study also shows that the distribution of NDM-1 and OXA-48 carbapenemases has increased since the first co-display in Türkiye and that IncHI1 is the first record in our country. This study provides an overview of the major plasmid families occurring in multiple antibiotic-resistant strains of K. pneumoniae. To our knowledge, this study represents the first report of IncHI1 record in Türkiye.

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Carbapenem and Colistin Resistance, Integrons and Plasmid Replicon Types in Multi-Drug Resistant Klebsiella Strains Isolated in Turkey

Cited by 2 publications

References 0 publications

Detection of Carbapenem Resistance Using the Genotypic and Phenotypic Methods in Klebsiella pneumoniae

Detection of Carbapenem Resistance Using the Genotypic and Phenotypic Methods in Klebsiella pneumoniae

Characterization of resistance genes and replicon typing in Carbapenem-resistant Klebsiella pneumoniae strains

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