Carbamino compounds of haemoglobin in human adult and foetal blood

Bauer, Christian; Schröder, E

doi:10.1113/jphysiol.1972.sp010042

Cited by 62 publications

(35 citation statements)

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“…The parameters to be put in and to be derived from the equations are summarized in Table 1 B together with the relations connecting these parameters. The carbamate content was independent of the PC02 (FERGUSON and ROUGHTON (1934), FERGUSON (1936), andSTADIE andO'BRIEN (1937) and showed 1.2 mmol/liter blood, on an average, which corresponded to the middle value between those of ROSSI- BERNARDI and ROUGHTON (1967) and BAUER and SCHRODER (1972). On the other hand, the calculated Donnan ratios for HC03-in the oxygenated and deoxygenated bloods coincided well with each other, as shown by FITZSIMONS and SENDROY (1961).…”

supporting

confidence: 58%

“…They also measured r(C1) in both the oxygenated and deoxygenated bloods, in which the value in the deoxygenated blood was obviously higher than that in the oxygenated blood. BAUER and SCHRODER (1972) determined the carbamate fraction in the red cell by subtracting from the intracellular bound C02 the intracellular bicarbonate, which was estimated from the extracellular bicarbonate by assuming r(HC03^)=r(Cl-). This assumption, however, seems invalid, referring to the data on r(Cl j (HENDERSON, 1928;FITzsIMONS and SENDROY, 1961) and on r(HC03) (FITzsIMONS and SENDROY, 1961, and Fig.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative analyses of the CO2 dissociation curve of oxygenated blood and the Haldane effect in human blood.

et al. 1983

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The theoretical equations for the C02 dissociation curve derived by MocHIZUKI et al. (1983) have made it possible to estimate the C02 contents in blood at any Pco2 by putting the intra-and extracellular bicarbonate contents at a certain Pco2 into them. Moreover, according to their Haldane effect equation, the carbamate and bicarbonate contributions are evaluated, when the Haldane effect and its plasma component are known along the PCo2 range. In order to accomplish the above calculation the water shifts due to the Pc02 and 02 saturation changes were measured as the changes of hematocrit. The hematocrit of oxygenated blood was linearly correlated to pH with a factor of -0.037, and the difference in hematocrit between oxygenated and deoxygenated bloods was 0.004 in terms of fractional hematocrit. The blood and plasma C02 contents measured at four different Pco2's were compared with the ones calculated by use of the intra-and extracellular bicarbonate contents at 42 Torr Pco2. The measured and calculated C02 contents coincided fairly well with each other. Using intra-and extracellular bicarbonate contents in oxygenated blood together with the Haldane effect and its plasma component, the carbamate contribution was then calculated. The carbamate content was about 1.2 mmol/liter blood over a Pcp2 range of 20 to 100 Torr, and its ratio to the total Haldane effect decreased from 50 to 40 %, as PC0, was increased. The ratio of the bicarbonate shift to the total bicarbonate change due to the Haldane effect, ranging from 0.82 to 0.66, was significantly greater than that measured by changing PCO2.

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confidence: 58%

Section: Discussionmentioning

confidence: 99%

Quantitative analyses of the CO2 dissociation curve of oxygenated blood and the Haldane effect in human blood.

et al. 1983

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“…Putting the measured plasma and red cell components of the Haldane effect into the equations of the Haldane effect, the carbamate and bicarbonate contributions in the Haldane effect were estimated. The obtained carbamate fraction was about 10% smaller than the value of ROSSI-BERNARDI and ROUGHTON (1967), but about 10% higher than that of BAUER and SCHRODER (1972).…”

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confidence: 93%

“…8. The parameter values used in the calcu- The above carbamate fraction, R, was referred to the experimental data of BAUER andSCHRODER (1972) andTAZAWA et al (1983). Assuming Cb(40) to be 44 vol %, (HC03-)c and (HC03-)p and i9c were determined as above by use of (C02)c/ (C02)p=0.45 and the relation of Fig.…”

Section: Equations For Carbamate Contribution In the Haldane Effectmentioning

confidence: 99%

“…The intracellular pH was calculated from the Henderson-Hasselbalch equations using the intracellular bicarbonate concentration, which was evaluated by subtracting carbamate content from the bound C02. The carbamate content of oxygenated blood was assumed to be 0.5 mmol/liter, blood independently of Pco2 according to BAUER and SCHRODER (1972). The intracellular bicarbonate change was calculated from the pH change by using the lc value ranging from 60 to 70 mmol/(liter RBC pH), where the j3p was kept constant at 7.5 mmol/(liter plasma rn pH) (TAKIWAKI et al, 1983).…”

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Derivation of theoretical equations of the CO2 dissociation curve and the carbamate fraction in the Haldane effect.

et al. 1983

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The simultaneous Henderson-Hasselbalch equations in plasma and red cell were solved in order to obtain the C02 dissociation curve of oxygenated blood. In order to solve the above two equations the following equation was added, in which the relationship between the intracellular (4pH) cand the extracellular pH change (dpHP) was defined as follows:pHc=(1+a)dpHP, where 1 +a is a factor to be determined from experimental data on Donnan's ratio for H+. From the solution, the ratio of bicarbonate shift to the C02 quantity released out of or combined with hemoglobin was calculated. The solution was validated by comparing the above ratio between the theoretical and experimental data. The C02 contents calculated at 12 Torr in whole blood, red cell, and plasma compartments show good agreement with the respective analyzed values. When the buffer values of hemoglobin and plasma buffer protein were 70.0 and 7.5 mmol/ (liter plasma • pH), respectively, a=-0.21 +0.05 • dpHP, and the Donnan's ratio for HC03-was assumed to be 0.7 at pH=7.33, the theoretical C02 dissociation curve fitted well with the experimental curve. The C02 dissociation curve of deoxygenated blood was expressed by adding the measured Haldane effect to the C02 content of oxygenated blood. This additive characteristic in turn made it possible to estimate carbamate contribution in the Haldane effect. In a previous paper (MocuIzuKI et al., 1982) it was clarified that the C02 dissociation curve could be expressed by an exponential function of PC02, whose exponent and multiplying factor had a consistent relation to each other. Therefore, a certain relation such as the Henderson-Hasselbalch equation was

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Hemoglobin Variants and the Rarer Hemoglobin Disorders

Kulozik¹

2006

Pediatric Hematology

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Carbamino compounds of haemoglobin in human adult and foetal blood

Cited by 62 publications

References 36 publications

Quantitative analyses of the CO2 dissociation curve of oxygenated blood and the Haldane effect in human blood.

Quantitative analyses of the CO2 dissociation curve of oxygenated blood and the Haldane effect in human blood.

Derivation of theoretical equations of the CO2 dissociation curve and the carbamate fraction in the Haldane effect.

Hemoglobin Variants and the Rarer Hemoglobin Disorders

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