Caracterización de levaduras<i>Debaryomyces hansenii</i>para el control biológico de la podredumbre azul del limón mexicano Characterization of yeast<i>Debaryomyces hansenii</i>for the biological control of blue mold decay of Mexican lemon

Hernández-Montiel, L. G.; Larralde-Corona, Claudia Patricia; Vero, Silvana; López-Aburto, MG; Ochoa, José Luís; Ascencio‐Valle, Felipe

doi:10.1080/19476330903080592

Cited by 15 publications

(6 citation statements)

References 35 publications

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“…In recent years, any bioactive compound and different samples from marine environments have been searched for. Many studies have shown that antagonistic bacterial communities associated with marine environments may have a beneficial role for plant protection [104]. However, little information has been found with respect to the study of microorganisms isolated from marine environments with the potential to control phytopathogens in postharvest, which could have agronomic potential compared to synthetic fungicides [105].…”

Section: Search For New Antagonistsmentioning

confidence: 99%

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

et al. 2019

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This review deals with the main mechanisms of action exerted by antagonistic bacteria, such as competition for space and nutrients, suppression via siderophores, hydrolytic enzymes, antibiosis, biofilm formation, and induction of plant resistance. These mechanisms inhibit phytopathogen growth that affects postharvest fruit since quality and safety parameters are influenced by the action of these microorganisms, which cause production losses in more than 50% of fruit tree species. The use of synthetic fungicide products has been the dominant control strategy for diseases caused by fungi. However, their excessive and inappropriate use in intensive agriculture has brought about problems that have led to environmental contamination, considerable residues in agricultural products, and phytopathogen resistance. Thus, there is a need to generate alternatives that are safe, ecological, and economically viable to face this problem. Phytopathogen inhibition in fruit utilizing antagonist microorganisms has been recognized as a type of biological control (BC), which could represent a viable and environmentally safe alternative to synthetic fungicides. Despite the ecological benefit that derives from the use of controllers and biological control agents (BCA) at a commercial level, their application and efficient use has been minimal at a global level.

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Section: Search For New Antagonistsmentioning

confidence: 99%

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

et al. 2019

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“…Incorporation of biocontrol microorganisms into the films could improve the control of postharvest diseases. In this regard, the capacity of the yeast Debaryomyces hansenii to control blue mold decay of lemon has been demonstrated [16]. Nevertheless, no research has been reported on the incorporation of microorganisms into oxidatively cross-linked WEAX films.…”

Section: Introductionmentioning

confidence: 99%

Covalently Cross-Linked Arabinoxylans Films for Debaryomyces hansenii Entrapment

et al. 2015

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“…A yeast aliquot (10 µL) was disposed on the gelled culture medium. The diameter of the inhibition halos of the fungal mycelial growth around the yeast colony was determined after 3-4 days of incubation at 25 • C [46]. Three replicates were performed, and the experiment was repeated three times.…”

Section: Determination Of Different Modes Of Action For Selected Yeas...mentioning

confidence: 99%

“…The cell wall preparations (CPWs) of B. cinerea were obtained with the methodology described by Saligkarias et al [47]. In minimal YNB medium (0.67% Yeast Nitrogen Base) supplemented with 1 mg mL −1 of the fungus wall, the yeast was cultured on a rotary shaker (100 rpm) at 25 • C [46]. Aliquots were taken from the culture 0, 1, 2, 3, 4, 6, 8, 10, 12 and 14 days post-inoculation.…”

Section: Secretion Of β-13-glucanase and Chitinase Activitymentioning

confidence: 99%

“…The enzyme-substrate mixture was incubated for 2 h at 40 • C. The reaction was then stopped with 200 µL of NaOH and the absorbance was measured with a spectrophotometer (Optizen POP BIO, Mecasys Co., Ltd., Daejeon, Republic of Korea) at 405 nm. The protein levels were determined with the abovementioned method, and enzymatic activity was expressed as µmol of p-nitrophenol per milligram protein per hour [46]. Three replicates were performed, and the experiment was repeated twice.…”

Section: Secretion Of β-13-glucanase and Chitinase Activitymentioning

confidence: 99%

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Indigenous Yeasts for the Biocontrol of Botrytis cinerea on Table Grapes in Chile

Sepúlveda

Vargas

Vero

et al. 2023

JoF

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One hundred twenty-five yeast strains isolated from table grapes and apples were evaluated for the control Botrytis cinerea of in vitro and in vivo. Ten strains were selected for their ability to inhibit mycelial growth of B. cinerea in vitro. In the in vivo assays, these yeasts were tested at 20 °C on ‘Thompson Seedless’ berries for 7 days; only three were selected (m11, me99 and ca80) because they significantly reduced the incidence of gray mold. These three yeast strains were then evaluated at different concentrations (1 × 107, 1 × 108 and 1 × 109 cells mL−1) on ‘Thompson Seedless’ grape berries at 20 °C. The strains m11, me99 and ca80 reduced the incidence of B. cinerea to 11.9, 26.1 and 32.1%, respectively, when the berries were submerged in a yeast suspension at a concentration of 1 × 109 cells mL−1 24 h before inoculation with B. cinerea. The most favorable pH for antifungal activity was 4.6 in the three isolates. The three yeast strains secreted the hydrolytic enzymes chitinase and β-1-glucanase, and two strains (me99 and ca80) produced siderophores. The three yeast strains exhibited low oxidative stress tolerance and only strain m11 had the ability to produce biofilms. The strains were identified using 5.8S-ITS rDNA PCR-RFLP and correspond to the Meyerozyma guilliermondii (m11) and Aureobasidium pullulans (me99 and ca80) species.

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Caracterización de levadurasDebaryomyces hanseniipara el control biológico de la podredumbre azul del limón mexicano Characterization of yeastDebaryomyces hanseniifor the biological control of blue mold decay of Mexican lemon

Cited by 15 publications

References 35 publications

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

Covalently Cross-Linked Arabinoxylans Films for Debaryomyces hansenii Entrapment

Indigenous Yeasts for the Biocontrol of Botrytis cinerea on Table Grapes in Chile

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