Caracterização molecular e bioquímica das enzimas envolvidas na biossíntese de prolina em &lt;i&gt;Trypanosoma cruzi&lt;/i&gt;.

Marchese, Letícia

doi:10.11606/t.42.2018.tde-02022018-151505

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…Noteworthy, cells that were allowed to recover in buffer containing Pro showed a tendence to have higher amounts of intracellular Pro (although this was not statistically significant), likely due to an increase in uptake stimulated by the nutritional stress (43)(44)(45). Remarkably, our results closely mirrored those obtained by Marchese (2017) in L. amazonensis, indicating that the biosynthesis of Pro from both Glu and Gln is not functional across Leishmania species.…”

Section: Braziliensis Does Not Utilize Glu To Restore the Intracellul...supporting

confidence: 53%

Genetic and functional dissection of the glutamate-proline pathway reveals a shortcut for glutamate catabolism inLeishmania

Campagnaro,

Cruz

2024

Preprint

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Trypanosomatids are early-divergent eukaryotes that have adapted to parasitism. During their life cycles, these parasites switch between a mammalian and an invertebrate host, and the ability to adapt their metabolism to different nutritional sources is detrimental for their success. In the invertebrate host, these protists have access to high amounts of amino acids and efficiently utilise it for energy production. Proline is a particularly efficient energy source for trypanosomes. Glutamate is also efficiently used byTrypanosoma cruzi, but it needs to be converted into proline prior to its catabolism. By employing a series of genetic modifications and functional analysis, we show here thatLeishmaniaparasites, the causative agents of leishmaniases, can utilise proline, glutamate and glutamine as energy sources, and although these parasites possess all the genes necessary for the biosynthesis of proline from glutamate, this pathway has, at best, limited function, with at least one of its components (pyrroline-5-carboxylate reductase) assuming divergent functions in different life cycle stages of the parasite. In fact, we show that the catabolism of glutamate is independent of proline biosynthesis and the former is most likely directly imported into the mitochondrion and catabolised to recover the cellular redox metabolism and increase mitochondrial membrane potential. Moreover, our data suggest a relevant role for glutamate dehydrogenase in nutritional stress response inLeishmania. These findings highlight relevant differences in amino acid metabolism betweenTrypanosomaandLeishmaniaand suggest a diversification in amino acid metabolic pathways within Trypanosomatidae.

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Section: Braziliensis Does Not Utilize Glu To Restore the Intracellul...supporting

confidence: 53%

Genetic and functional dissection of the glutamate-proline pathway reveals a shortcut for glutamate catabolism inLeishmania

Campagnaro,

Cruz

2024

Preprint

View full text Add to dashboard Cite

show abstract

Caracterização molecular e bioquímica das enzimas envolvidas na biossíntese de prolina em <i>Trypanosoma cruzi</i>.

Cited by 1 publication

References 17 publications

Genetic and functional dissection of the glutamate-proline pathway reveals a shortcut for glutamate catabolism inLeishmania

Genetic and functional dissection of the glutamate-proline pathway reveals a shortcut for glutamate catabolism inLeishmania

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